References to Ocimum Spoligotyping kit


spoligotypingOcimum acquired the spoligotyping kit through an acquisition of the Biomolecules division of Isogen Lifesciences based in The Netherlands. Since the acquisition in 2006, Ocimum has continued to invest in additional R&D and is in the process of adding several new kits to its portfolio.

As with other products, below is a chronological publication reference to the spoligotyping kit. The earliest publication for this product is dated way back in 1997. Many publications will refer to Isogen as that was the name of our subsidiary. Recently we changed the name back to Ocimum Biosolutions but we continue to find references to Isogen.

With the large number of references of over 400 and from labs across the world, the spoligotyping kit has been validated for over 15 years now giving confidence to researchers across the globe using it.

Updated: March 31 2013

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2013

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Spoligotyping of multidrug-resistant Mycobacterium tuberculosis isolates in Ethiopia

B Diriba, T Berkessa, G Mamo, Y Tedla… – … International Journal of …, 2013 – ingentaconnect.com
 al.10 and as per the spoligotype kit supplier’s instructions (Ocimum Biosolutions, Ijssel- stein 
clinical isolates were represented by a unique (non-clustered) spoligotype pattern  these patterns,
which are already recorded in the fourth international spoligotyping database (SpolDB4 

Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe

A Mihret, Y Bekele, M Aytenew, M Abebe, L Wassie… – African health …, 2013 – ajol.info
 was carried out using the commercially available kit from Ocimum Biosolutions, India, using  results
with already existing designations in the international spoligotyping database, SpolDB4  5. In this
database, two or more patient isolates sharing identical spoligotype patterns are 

[HTML] Admixed Phylogenetic Distribution of Drug Resistant Mycobacterium tuberculosis in Saudi Arabia

B Varghese, P Supply, C Allix-Béguec, M Shoukri… – PloS one, 2013 – dx.plos.org
 grouping of the isolates with a typical Beijing spoligotype) (Figure S1).  were identified based on
isolates sharing identical MIRU-VNTR types and spoligotypes loci based MIRU-VNTR typing
(Genoscreen, Lille, France) and Spoligotyping (Ocimum Biosolutions, Hyderabad, India 

[PDF] Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools

B Deresa, FJ Conraths, G Ameni… – Acta Veterinaria …, 2013 – actavetscand.com
 Spoligotyping Spoligotyping was performed at ALIPB; following the procedure
described earlier [28] and according to the spoligotype kit supplier’s instructions
(Ocimum Biosolutions Company, Iisselstein, The Netherlands). 

Molecular typing of mycobacteria isolated from extrapulmonary tuberculosis patients at Debre Birhan Referral Hospital, central Ethiopia

L Garedew, A Mihret, T Abebe… – … Journal of Infectious …, 2013 – informahealthcare.com
 The 34 isolates confirmed to be M. tuberculosis by deletion typing were further characterized
by spoligotyping techniques, as previously described [11,12], and in accordance with the
spoligotype kit supplier instructions (Ocimum Biosolutions Company, IJsselstein 

Endogenous reactivation followed by exogenous re-infection with drug resistant strains, a new challenge for tuberculosis control in Saudi Arabia

B Varghese, R al-Omari, C Grimshaw, S Al-Hajoj – Tuberculosis, 2013 – Elsevier
 All of the available isolates from patients were subjected to spoligotyping (Ocimum Biosolutions,
Hyderabad, India) and 24 loci based MIRU-VNTR typing (Genoscreen, Lille, France) by using
the commercially available kits and methods described previously. 

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2012

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Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria using a microarray system with automatic data processing and assignment

A Ruettger, J Nieter, A Skrypnyk… – Journal of Clinical …, 2012 – Am Soc Microbiol

 Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum
Biosolutions, Hydera- 79 bad, India) and the present DNA microarray.  ArrayStrip spoligotyping
and the assay’s capability of identifying new spoligotypes and li- 109 neages. 

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Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin

MI Millán-Lou, H Alonso, P Gavín… – Journal of Clinical …, 2012 – Am Soc Microbiol

 First, all 292 isolates were screened by spoligotyping with a commercially available
kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al.
(14). Seventy of the 292 isolates belonged to the Beijing family. 

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GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… – European …, 2012 – Eur Respiratory Soc

 Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the
Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer’s
instructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web 

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New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier

 Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotypingSpoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). 

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 [HTML] DISTRIBUTION OF SPOLIGOTYPING DEFINED GENOTYPIC LINEAGES AMONG DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES IN ANKARA, TURKEY

[HTML] from plos.org

O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… – PloS one, 2012 – dx.plos.org

 Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands  tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.
Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database 

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[HTML] SPOLIGOTYPING AND DRUG RESISTANCE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM NATIONAL SURVEY IN CHINA

[HTML] from plos.org

Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… – PloS one, 2012 – dx.plos.org

 A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as
described by  of the direct repeat region of Mycobacterium tuberculosis: implications for
interpretation of spoligotyping data.  2004) Predominant tuberculosis spoligotypes, Delhi, India. 

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[HTML] SPOLIGOTYPING AND VARIABLE NUMBER TANDEM REPEAT ANALYSIS OF MYCOBACTERIUM BOVIS ISOLATES FROM CATTLE IN BRAZIL

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PM Parreiras, GI Andrade… – Memórias do Instituto …, 2012 – SciELO Brasil

 The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43
oligonucleotides of known spacer sequences by reversed-line blot hybridisation.  Spoligotyping –
Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 

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EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI

V Vadwai, A Shetty, P Supply… – Tuberculosis, 2012 – Elsevier

 Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the  13/69,
18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at  Analysis
of the congruence between MIRU-VNTR and spoligotyping results combined with the 

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EPIDEMIOLOGICAL SIGNIFICANCE OF THE DOMESTIC BLACK PIG (SUS SCROFA) IN THE MAINTENANCE OF BOVINE TUBERCULOSIS IN SICILY

V Di Marco, P Mazzone, MT Capucchio… – Journal of Clinical …, 2012 – Am Soc Microbiol

 direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 
M. bovis isolates from both field studies were genotyped by both spoligotyping 342  Four
spoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 

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TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS

E Augustynowicz-Kopeć, T Jagielski, M Kozińska… – Journal of Infection, 2012 – Elsevier

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen 
had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU  Assuming
isolates revealing both identical spoligotypes and VNTR profiles are representing the 

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[PDF] PREVALENCE OF PULMONARY TB AND SPOLIGOTYPE PATTERN OF MYCOBACTERIUM TUBERCULOSIS AMONG TB SUSPECTS IN A RURAL COMMUNITY IN SOUTHWEST ETHIOPIA

[PDF] from biomedcentral.com

A Deribew, G Abebe, L Apers… – BMC Infectious …, 2012 – biomedcentral.com

 Standard spoligotyping method [12] was done generally as described by Kamerbeek and
colleagues using a commercially available kit (Isogen Bioscience BV  Spoligotypes described
only once (non-clustered) in this study and in the SpolDB4 were designated as 

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CHARACTERIZING< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM KARACHI, PAKISTAN: DRUG RESISTANCE AND GENOTYPES

A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… – International Journal of …, 2012 – Elsevier

 The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the
World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18.  3.4. Genotypic
pattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped

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IMPLICATION OF THE RD< SUP> RIO</SUP>< I> MYCOBACTERIUM TUBERCULOSIS</I> SUBLINEAGE IN MULTIDRUG RESISTANT TUBERCULOSIS IN PORTUGAL

S David, EL Duarte, CQF Leite, JN Ribeiro… – Infection, Genetics and …, 2012 – Elsevier

 patient), were genotyped by spoligotyping according to the manufacturer’s instructions (Isogen
Bioscience BV  Contribution of Spoligotyping to the Characterization of the population structure
of  Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the 

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[PDF] ÇUKUROVA BÖLGESINDE AKCIĞER TÜBERKÜLOZLU HASTALARDAN İZOLE EDILEN MYCOBACTERIUM TUBERCULOSIS SUŞLARININ SPOLIGOTIPLENDIRME VE MIRU-VNTR YÖNTEMIYLE …

[PDF] from mikrobiyolbul.org

ÜO ZEYTİNLİ… – Mikrobiyol Bul, 2012 – mikrobiyolbul.org

 Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology;
Turkey. GİRİŞ  Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri
(Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. 

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2011

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[HTML] Bovine tuberculosis in a Nebraska herd of farmed elk and fallow deer: a failure of the tuberculin skin test and opportunities for serodiagnosis

[HTML] from hindawi.com

W Waters, GE Stevens, MA Schoenbaum… – Veterinary medicine …, 2011 – hindawi.com

 Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleic
acid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (Ocimum
Biosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. 

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 [HTML] The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines …

[HTML] from biomedcentral.com

E Abadia, J Zhang, V Ritacco… – BMC infectious …, 2011 – biomedcentral.com

 of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluate
the benefits of a new platform regarding the classical one on these samples. Most centers had
performed spoligotyping using commercial membranes (Ocimum, Hyderabad, India 

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Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterium reference laboratory

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer

 for spoli- gotyping results, standardization and reproducibility depend on the quality of the
commercial spoligotyping kit (Ocimum).  Spoligotyping Pattern  Both PETRA (a MIRU-VNTR lineage
with central asian spoligotypes, CAS) and Beijing clusters have been observed in other 

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DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …

S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier

 commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now Ocimum
Biosolutions) and  Owing to small sample size, the exact prevalence of various 272 spoligotypes
and M  Out of the isolates clustered by spoligotyping, all 280 were unique strains by 

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New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier

 Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotypingSpoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). 

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High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced immunodeficiency: implications for TB screening

[HTML] from nih.gov

T Oni, R Burke, R Tsekela, N Bangani, R Seldon… – Thorax, 2011 – thorax.highwire.org

 Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotyping
as previously described4 using a commercially available kit from Ocimum Biosolutions
BV, The Netherlands. The hybridisation patterns obtained 

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[HTML] Pathology of camel tuberculosis and molecular characterization of its causative agents in pastoral regions of Ethiopia

[HTML] from plos.org

G Mamo, G Bayleyegn, TS Tessema, M Legesse… – PloS one, 2011 – dx.plos.org

 SpoligotypingSpoligotyping was performed as previously described by Kamerbeek
and coauthors [22] and according to the spoligotype kit supplier’s instructions (Ocimum
Biosolutions Company, Iisselstein, The Netherlands). 

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POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA

Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… – Tuberculosis, 2011 – Elsevier

 Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The
Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the
SpoligDB4 database and assigned a spoligotyping international type (SIT). 

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[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED

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R Groenheit, S Ghebremichael, J Svensson… – PLoS One, 2011 – dx.plos.org

 All isolates that were subjected to RFLP were also spoligotyped Altogether, 414 isolates were
available for spoligotyping, of which 336 isolates were grouped into 42 clusters  Out of 229 isolates,
82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into 

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GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA

[PDF] from ajol.info

J Bazira, M Matte, BB Asiimwe… – African health sciences, 2011 – ajol.info

 was performed as previously described29 using a commercially available kit (Isogen Bioscience
BV  Spoligotypes that did not match any existing pattern in the SpolDB4 database were  tuberculosis
within the Mbarara, we have used RD analysis and spoligotyping to characterize 

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FLUOROQUINOLONE RESISTANCE AMONG MYCOBACTERIUM TUBERCULOSIS STRAINS FROM KARACHI, PAKISTAN: DATA FROM COMMUNITY-BASED FIELD CLINICS

[HTML] from nih.gov

Y Rafiq, K Jabeen, R Hasan, S Jafri… – Antimicrobial agents …, 2011 – Am Soc Microbiol

 was performed on all FQ-resistant strains using a commercially available kit provided by Isogen
Life Science BV  The spoligotypes were compared with the most prevalent M. tuberculosis
subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of 

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[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN

[HTML] from biomedcentral.com

YC Chiu, SF Huang, KW Yu, YC Lee… – BMC infectious …, 2011 – biomedcentral.com

 Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen
Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as deletion from spacer
1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. 

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[HTML] INITIAL PRESENTATIONS PREDICT MORTALITY IN PULMONARY TUBERCULOSIS PATIENTS-A PROSPECTIVE OBSERVATIONAL STUDY

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JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… – PloS one, 2011 – dx.plos.org

 All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience
BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. 

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[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA

[HTML] from nih.gov

X Gonzalo, M Ambroggi, E Cordova… – Emerging Infectious …, 2011 – ncbi.nlm.nih.gov

 DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according
to the manufacturer’s instructions (Isogen Life Science, IJsselstein, the Netherlands); and data
were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). 

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CHARACTERISTICS OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN TAIWAN: A POPULATION-BASED STUDY

CW Chang, MH Wu, PC Chuang… – Infection, Genetics and Evolution, 2011 – Elsevier

 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15
Netherlands) was used as  Even through spoligotyping results 3 revealed the genetic diversities
of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not 

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MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF BOVINE …

[PDF] from uaemex.mx

IF Padilla-Campos, JC Segura-Correa… – Tropical and …, 2011 – redalyc.uaemex.mx

 Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors.  Spoligotyping was carried
out following the protocol described by Kamerbeek et al  sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The 

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[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE

[HTML] from biomedcentral.com

C Gortazar, MJ Torres, P Acevedo, J Aznar… – BMC …, 2011 – biomedcentral.com

 Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified
product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). 

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MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF …

[PDF] from uady.mx

IF Padilla, JC Segura, JC Rodriguez… – Tropical and …, 2011 – ccba.uady.mx

 Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors.  Spoligotyping was carried
out following the protocol described by Kamerbeek et al  sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The 

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EPIDEMIOLOGICAL TRENDS AND CLINICAL COMPARISONS OF< I> MYCOBACTERIUM</I>< I> TUBERCULOSIS</I> LINEAGES IN THAI TB MENINGITIS

K Faksri, F Drobniewski, V Nikolayevskyy, T Brown… – Tuberculosis, 2011 – Elsevier

 isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns
were available) and spoligotyping results (141 spoligotypes were available  27 Spoligotyping
was performed according to the manufacturer’s instructions (Isogen Bioscience BV 

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A NOVEL METHOD FOR THE RAPID AND PROSPECTIVE IDENTIFICATION OF BEIJING MYCOBACTERIUM TUBERCULOSIS STRAINS BY HIGH‐RESOLUTION MELTING ANALYSIS

M Alonso, Y Navarro, F Barletta… – Clinical …, 2011 – Wiley Online Library

 Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of
the non-Beijing genotype, according to their spoligotypes) were grown on  Spoligotyping was
performed in accordance with the manufacturer’s instructions (Isogen, De Meern, the 

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QUALITY ASSURANCE FOR MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS METHODS IN THE MYCOBACTERIUM REFERENCE LABORATORY

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer

 43 spacer spoligotyping was performed according to the manufacturer’s instructions
(Isogen/Ocimun) as described previously.  27 Spoligotyping Pattern  Both PETRA (a MIRU-VNTR
lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other 

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EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLAND

M Krawczyk, A Brzostek, A Gorna… – … of Tuberculosis and …, 2011 – ingentaconnect.com

 of van Embden et al.16 Spoligotyping was performed as described by Kamerbeek et al., using
the Isogen Life Science kit  Spoligotyping analysis showed that 184 (78.6%) of the strains were
grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes

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ONE YEAR NATIONWIDE EVALUATION OF 24-LOCUS MIRU-VNTR GENOTYPING ON SLOVENIAN< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES

U Bidovec-Stojkovic, M Zolnir-Dovc… – Respiratory Medicine, 2011 – Elsevier

 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV 
identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping
jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only 

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MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) ISOLATED FROM CATTLE IN NORTHEAST AND NORTHWEST CHINA

Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… – Research in veterinary …, 2011 – Elsevier

 2.5. SpoligotypingSpoligotyping kits and Mini-blotter MN45 were obtained from Isogen
Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was
produced by Roche Applied Science Co. (Penzberg, Germany). 

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[HTML] TRANSMISSION PATTERN OF DRUG-RESISTANT TUBERCULOSIS AND ITS IMPLICATION FOR TUBERCULOSIS CONTROL IN EASTERN RURAL CHINA

[HTML] from plos.org

Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… – PloS one, 2011 – dx.plos.org

 Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience
BV  b Probaiblity that the Spoligotyping pattern belongs to the clades.  N, Bennett KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

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SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA

US Rajapaksa… – Indian Journal of Microbiology, 2011 – Springer

 Spoligotyping was carried out according to manufacturer’s guidelines using a
commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-
lates that confirmed to be Beijing strain were further characterised. 

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DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …

S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier

 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences ,
BV  Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.
tuberculosis  Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 

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MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA

AO Jenkins, SIB Cadmus, EH Venter… – Veterinary …, 2011 – Elsevier

 Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and
13 M  None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were 
All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical 

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GENETIC DIVERSITY OF THE MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY BASED ON IS6110, SNP, LSP AND VNTR PROFILES FROM THAILAND

K Faksri, F Drobniewski, V Nikolayevskyy… – Infection, Genetics and …, 2011 – Elsevier

 Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns were
available) and spoligotyping results (all 80 spoligotypes were available  Spoligotyping was
performed according to the manufacturer s instructions (Isogen Bioscience BV 

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[PDF] 骨结核临床分离菌株基因分型的特点

[PDF] from clinicmed.net

李文胜, 刘毅, 孙勇, 田苗, 刘忠泉… – 中华临床医师杂志 ( …, 2011 – clinicmed.net

 TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaKaRa 公司。
凝胶成像仪购自FOTO DYNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN45为荷兰
Isogen Bioscience 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 

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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES

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A Saedi… – 2011 – qmro.qmul.ac.uk

 130 3.2.11 Identification of the UAE isolates by spoligotyping….. 132 3.2.12 Comparison between
MIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ….. 

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[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA

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S Barandiaran, EV Moras, A Adri& – Veterinary Medicine …, 2011 – hindawi.com

 Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun 
Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment 
isolates were defined as two or more M. bovis strains with identical spoligotypes

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[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL

[HTML] from scielo.br

SS Miranda, WS Carvalho, PN Suffys… – Memórias do Instituto …, 2011 – SciELO Brasil

 was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using
a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen
Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary 

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EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINA

Z Sun, R Cao, M Tian, X Zhang, X Zhang… – Research in Veterinary …, 2011 – Elsevier

 Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci
(ETR  Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following
the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV 

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[HTML] MYCOBACTERIUM TUBERCULOSIS SPOLIGOTYPES AND DRUG SUSCEPTIBILITY PATTERN OF ISOLATES FROM TUBERCULOSIS PATIENTS IN SOUTH-WESTERN UGANDA

[HTML] from biomedcentral.com

J Bazira, BB Asiimwe, ML Joloba… – BMC infectious …, 2011 – biomedcentral.com

 Spoligotyping. Standard spoligotyping was performed as previously described [29] using a
commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands).  Spoligotyping.
All the isolates had spoligotypes characteristic of M. tuberculosis. 

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A FIRST INSIGHT ON THE POPULATION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS COMPLEX AS STUDIED BYSPOLIGOTYPING AND MIRU-VNTRS IN BOGOTÁ, COLOMBIA

I Cerezo, Y Jiménez, J Hernandez, T Zozio… – Infection, Genetics and …, 2011 – Elsevier

 Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the 
Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes
and MIRU  was used to build minimum spanning trees based on spoligotyping and MIRU 

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[PDF] PRELIMINARY MAPPING STUDY OF MYCOBACTERIUM TUBERCULOSIS’ COMPLEX DNA BYSPOLIGOTYPING ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT …

[PDF] from digitaljournals.org

V Lisdawati, I Parwati… – Bulletin of Health …, 2011 – indonesia.digitaljournals.org

 PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian
pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen
Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. 

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[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS

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A Balseiro, O Rodríguez, P González-Quirós… – prevalence, 2011 – uclm.es

 al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR
products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by
spoligotyping 127 (Kamerbeek et al., 1997). Results were 

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CLINICAL AND MICROBIOLOGICAL CHARACTERISTICS OF URINE CULTURE-CONFIRMED GENITOURINARY TUBERCULOSIS AT MEDICAL CENTERS IN TAIWAN FROM 1995 TO 2007

HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… – European journal of …, 2011 – Springer

 commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as described by the manufacturer. The fourth international spoligotyping database
(SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 

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MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATION

MV Afanas’ ev, LN Ikryannikova, EN Il’ina… – European Journal of …, 2011 – Springer

 spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur
J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered
in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. 

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ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS

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WJ Su, JY Feng, YC Chiu… – European Respiratory …, 2011 – Eur Respiratory Soc

 All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV,
Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least three of) spacers 35–43. 

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[PDF] A TWO-STEP STRATEGY FOR MOLECULAR TYPING OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM POLAND.

[PDF] from microbiology.pl

T JAGIELSKI, EWAA KOPEĆ… – Polish journal of …, 2011 – pjm.microbiology.pl

 Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV  e isolates
whose spoligotype patterns were already recorded in the database, were assigned “shared types”,
whereas those of spoligotypes identified for the first time, were 

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MOLECULAR TECHNOLOGIES USED IN DETECTING OF SENSITIVE AND ISONIAZID-RESISTANT MYCOBACTERIUM TUBERCULOSIS

OY Limanskaya… – Cytology and Genetics, 2011 – Springer

 The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian
Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl
(pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA 

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STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE

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FS Spies, AW Ribeiro, DF Ramos… – Journal of clinical …, 2011 – Am Soc Microbiol

 Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV,
Netherlands  it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non 
Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since 

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GENOTYPING AND DRUG RESISTANCE PATTERNS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN FIVE PROVINCES OF CHINA

YL Guo, Y Liu, SM Wang, CY Li… – … of Tuberculosis and …, 2011 – ingentaconnect.com

 Of the (Isogen Bioscience BV, De Meern, The Netherlands).  1–1800 = shared spoligotypes. Table
3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number
IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU 

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ASSOCIATION OF< I> MYCOBACTERIUM TUBERCULOSIS</I> GENOTYPES AND CLINICAL AND EPIDEMIOLOGICAL FEATURES-A MULTI-CENTER STUDY IN TAIWAN

SF Huang, WJ Su, HY Dou, JY Feng… – Infection, Genetics and …, 2011 – Elsevier

 Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek
et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were
identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT 

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THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS

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LI Wen-sheng, LIU Yi, SUN Yong… – Chinese Journal of …, 2011 – global.clinicmed.net

 TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaKaRa 公司。
凝胶成像仪购自 FOTO DYNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN45为荷兰
Isogen Bioscience 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 

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MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE

EES Figueiredo, DF Ramos, L Medeiros… – The Veterinary …, 2011 – Elsevier

 to the spoligo-membrane was performed according to the manufacturer’s instructions (Isogen
Life Sciences).  The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm
clonal relationships  Four spoligotypes were identified among the 12 M. bovis isolates. 

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DETERMINAÇÃO DO CUSTO BIOLÓGICO EM ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS COM MUTAÇÕES NOS GENES RPSL, RRS, GIDB, RPOB E KATG

[PDF] from ufrgs.br

FS Spies – 2011 – lume.ufrgs.br

 restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e
unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- 
Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região 

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A MAJOR SUBGROUP OF BEIJING FAMILY MYCOBACTERIUM TUBERCULOSIS IS ASSOCIATED WITH MULTIDRUG RESISTANCE AND INCREASED TRANSMISSIBILITY

Y Hu, X Ma, EA Graviss, W Wang… – Epidemiology and …, 2011 – Cambridge Univ Press

 Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen 
0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243  Thirty-three
(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that 

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[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA

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SK Thomas, CC Iravatham, BH Moni, A Kumar… – PloS one, 2011 – dx.plos.org

 the help of commercially available line probe arrays (Isogen Biosciences BV  tuberculosis complex
genetic diversity: mining the fourth international spoligotyping database (SpolDB4  KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

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[HTML] TUBERCULOSIS IN SUDAN: A STUDY OF MYCOBACTERIUM TUBERCULOSIS STRAIN GENOTYPE AND SUSCEPTIBILITY TO ANTI-TUBERCULOSIS DRUGS

[HTML] from biomedcentral.com

GSS Eldin, I Fadl-Elmula, MS Ali… – BMC infectious …, 2011 – biomedcentral.com

 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The  Spoligotyping
provided a relatively simple and rapid means of describing the population of  improved
discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU 

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[PDF] MOLECULAR CHARACTERISATION OF MYCOBACTERIUM BOVIS ISOLATED FROM AFRICAN BUFFALOES (SYNCERUS CAFFER) IN HLUHLUWE-IMFOLOZI PARK IN KWAZULU-NATAL, SOUTH AFRICA

[PDF] from scielo.org.za

TM Hlokwe, AO Jenkins, EM Streicher… – … Journal of Veterinary …, 2011 – scielo.org.za

 One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates 
Spoligotyping Spoligotyping was done according to a standardised international method
(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). 

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GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL

[PDF] from redetb.org

A Rocha, AR Elias, LF Sobral, DF Soares… – Tuberculosis, 2011 – Elsevier

 using PCR and hybridization conditions on a commercially available membrane (Isogen
Bioscience BV  All isolates that yielded spoligotypes that were recognized as not previously
described patterns were submitted to at least one additional spoligotyping assay and 

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MOLECULAR AND EPIDEMIOLOGICAL CHARACTERIZATION OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES IN JOHANNESBURG, SOUTH AFRICA

[PDF] from wits.ac.za

CK Mlambo – 2011 – wiredspace.wits.ac.za

 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and
corresponding spoligotyping-based information (octal code, SIT, and clade)…………
64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 

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USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATION

B Nivin, J Driscoll, T Glaser, P Bifani… – Infection control and …, 2000 – JSTOR

 The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience
BV  In several cases of identical spoligotypes, the Wadsworth Center indicated that the 
Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP 

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CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA

L Xing, R Liu, Q Li, Z Peng… – European Journal of Clinical …, 2011 – Springer

 Of 105 isolates spoligotyped, the major spoligotypes found were  Spoligotyping was performed
according to the internation- ally standardized protocol [5]. A commercially available spoligotyping
kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described 

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TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEP

M Muņoz Mendoza, L Juan, S Menéndez… – The Veterinary …, 2011 – Elsevier

 by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV).  Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and
M. bovis  close contact with cattle or goats infected by the same spoligotypes (Table 1 

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[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL

[HTML] from scielo.br

SSS Higino, SR Pinheiro, GO Souza… – Brazilian Journal of …, 2011 – SciELO Brasil

 Isolate was characterised by spoligotyping as described (7). The amplified product
was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). 

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EPIDEMIOLOGICAL STUDIES OF BEIJING STRAINS OF MYCOBACTERIUM TUBERCULOSIS FROM TAIPEI AND OTHER ASIAN CITIES BASED ON MIRU PROFILES

JUNREN SUN, HYUN DOU, SYI LEE… – Apmis, 2011 – Wiley Online Library

 on isolates from areas where the Beijing strain is prevalent as most isolates in this family share
the same spoligotypes (1, 5  Spoligotyping  to the manufacturers’ protocol, to a set of 43 oligonucle-
otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit 

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*************************************************************

2010

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Comparison of restriction fragment length polymorphism with the polymorphic guanine-cytosine-rich sequence and spoligotyping for differentiation of Mycobacterium …

[HTML] from nih.gov

L Flores, LG Jarlsberg, EY Kim… – Journal of clinical …, 2010 – Am Soc Microbiol

 2004 was performed with standardized methods (15) using a membrane with 43 oligonucleotides
representing the different direct variable repeats (DVRs) immobilized in specific locations (Ocimum
Biosolutions, Inc., Gaithersburg, MD). Beginning in 2004, spoligotyping of the 

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Fitness study of the RDRio lineage and Latin American–Mediterranean family of Mycobacterium tuberculosis in the city of Rio Grande, Brazil

A Von Groll, A Martin, C Felix… – FEMS Immunology …, 2010 – Wiley Online Library

 RD Rio BrgR: 5′-CACCGCCACGCTGAATGAGACCA-3′, –. Genotyping by
spoligotypingSpoligotyping was performed with a commercial kit (Ocimum Biosolutions
BV, India) according to standard procedures (Kamerbeek et al., 1997). 

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[HTML] Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype

[HTML] from plos.org

A Von Groll, A Martin, M Stehr, M Singh, F Portaels… – PloS one, 2010 – dx.plos.org

 Strains were fully characterized and analyzed for the presence of mutations conferring resistance
to RMP and INH. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions BV,
India) according to standard procedures [29]. Molecular characterization. 

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[HTML] MOLECULAR DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PATIENTS WITH PULMONARY TUBERCULOSIS IN MOZAMBIQUE

[HTML] from biomedcentral.com

SO Viegas, A Machado, R Groenheit… – BMC …, 2010 – biomedcentral.com

 using a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands).
Spoligotyping results were analysed with the BioNumerics Software ver. 5.01 (Applied Maths,
Kortrijk, Belgium). Database comparison and geographical distribution of spoligotypes

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[PDF] ASSOCIATION AMONG THE RV2629 GENE, RPOB GENE, RFP RESISTANCE AND BEIJING GENOTYPE IN M. TUBERCULOSIS CLINICAL ISOLATES

[PDF] from academicjournals.org

J Zhang, X Wu, Y Yang, Y Zhuang… – African Journal of …, 2010 – academicjournals.org

 The amplified PCR products were hybridized with spoligotyping Biodyne C membrane immobilized
43 oligonucleotide probes (Isogen Life Science, Holland).  A total of 8 distinct spoligotyping patterns
were observed.  7 isolates had unique spoligotypes (Figure 1, Tables 1 and 2 

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SINGLE NUCLEOTIDE POLYMORPHISMS IN CELL WALL BIOSYNTHESIS-ASSOCIATED GENES AND PHYLOGENY OF< I> MYCOBACTERIUM TUBERCULOSIS</I> LINEAGES

PC Chuang, YMA Chen, HY Chen… – Infection, Genetics and …, 2010 – Elsevier

 2.2. Genotype analysis. Spoligotyping using a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands  Spoligotypes and RFLP patterns were scanned and analyzed
with Bionumerics ® software (version 4.601; Applied Maths, Kortrijk, Belgium). 

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[HTML] ASSOCIATIONS BETWEEN MYCOBACTERIUM TUBERCULOSIS STRAINS AND PHENOTYPES

[HTML] from nih.gov

T Brown, V Nikolayevskyy, P Velji… – Emerging Infectious …, 2010 – ncbi.nlm.nih.gov

 Spoligotyping was performed according to the manufacturer’s instructions (Isogen Lifescience,
IJsselstein, the Netherlands) (9). Images were digitized and entered into BioNumerics software
by using the BNIMA module (Applied Maths). Spoligotypes were assigned to families 

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[HTML] SENSIBILIDAD Y ESPECIFICIDAD DE PCR ANIDADA Y SPOLIGOTYPING COMO PRUEBAS RÁPIDAS DE DIAGNÓSTICO DE TUBERCULOSIS BOVINA EN TEJIDO FRESCO

[HTML] from scielo.org.mx

F Milián Suazo, B Harris, C Arriaga Díaz… – Revista mexicana de …, 2010 – scielo.org.mx

 Spoligotyping se realizó tal como lo describe Kamerbeek et al (17) . De manera
resumida, la amplificación del  por 30 seg. La hibridización se realizó de acuerdo
a las recomendaciones del fabricante (Isogen). El ADN hibridado 

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[HTML] GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM ALOW-ENDEMIC SETTING IN NORTHWESTERN STATE OF PARANÁ IN SOUTHERN BRAZIL

[HTML] from scielo.br

EN Noguti, CQF Leite, AC Malaspina… – Memórias do Instituto …, 2010 – SciELO Brasil

 Spoligotyping – Spoligotyping was performed in all 93 M. tuberculosis isolates, using the standard
method  of 43 spacer oligonucleotides covalently linked to the spoligo-membrane (Isogen Life
Sciences  Spoligotypes were reported using an octal code in which the 43-digit binary 

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[HTML] EXTENSIVE TRANSMISSION OF ISONIAZID RESISTANT M. TUBERCULOSIS AND ITS ASSOCIATION WITH INCREASED MULTIDRUG-RESISTANT TB IN TWO RURAL COUNTIES OF EASTERN CHINA: A …

[HTML] from biomedcentral.com

Y Hu, S Hoffner, W Jiang… – BMC infectious …, 2010 – biomedcentral.com

 Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV  Table 3.
Spoligotyping pattern and frequency of the MTB isolates included.  B, Kurepina N, Bennett KP:
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

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[HTML] MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES FROM ETHIOPIAN CATTLE

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D Biffa, E Skjerve, J Oloya, A Bogale… – BMC veterinary …, 2010 – biomedcentral.com

 spacers were then hybridized with the known oligonucleotide spacers ligated to the membrane
(Isogen).  The discriminatory power (HGDI) of the spoligotyping method was calculated to be 0.81.
Also on the basis of the above findings (the number of distinct spoligotypes (S) = 12 

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[HTML] MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN, BAMAKO, MALI

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B Diarra, S Siddiqui, D Sogoba… – Emerging infectious …, 2010 – ncbi.nlm.nih.gov

 See the article”Global Distribution of Mycobacterium tuberculosis Spoligotypes” in volume 8 on
page 1347.  Spoligotyping using a commercially available kit (Spoligotyping Isogen Life Science,
De Meern, the Netherlands) showed characteristics of the Beijing clade (Appendix 

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EVALUATION OF 24 LOCUS MIRU-VNTR GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN CANADA

S Christianson, J Wolfe, P Orr, J Karlowsky… – Tuberculosis, 2010 – Elsevier

 some cases. 22 Blots were purchased from Isogen Biosolutions and run according
to the manufacturer’s directions. Spoligotyping patterns were converted to the “octal”
pattern using the previously described formula. 23 Data 

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MYCOBACTERIUM MICROTI INFECTION ASSOCIATED WITH SPINDLE CELL PSEUDOTUMOUR AND HYPERCALCAEMIA: A POSSIBLE LINK WITH AN INFECTED ALPACA

C McGoldrick, C Coghlin, A Seagar… – BMJ Case …, 2010 – casereports.bmj.com

 At SMRL spoligotyping was performed using a commercial assay available from Isogen Life
Science (3600 BT; Maarssen, The Netherlands) and 15-locus Mycobacterial Interspersed
Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing, performed using the 

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MOLECULAR EPIDEMIOLOGY OF HUMAN CASES OF TUBERCULOSIS BY MYCOBACTERIUM BOVIS IN MEXICO

F Milián-Suazo, L Pérez-Guerrero… – Preventive veterinary …, 2010 – Elsevier

 Coding allows easy comparisons of spoligotypes from different laboratories and from different
parts of  Spoligotyping was performed following a standard protocol (Kemerbeek et al., 1997).  6
spacer sequences from M. bovis BCG were covalently bound (ISOGEN, Maarssen, the 

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THE MOST PREDOMINANT SPOLIGOPATTERNS OF< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES AMONG IRANIAN, AFGHAN-IMMIGRANT, PAKISTANI AND TURKISH TUBERCULOSIS PATIENTS: …

MA Merza, P Farnia, AM Salih, MR Masjedi… – …, 2010 – content.karger.com

 [19] , with a commercially available kit, according to the manufacturer’s instructions (Isogen Biosci-
ence, The  Analysis of Spoligotyping Data A total of 133 distinct spoligopatterns were identi- fied 
The distribution of spoligotypes (T, CAS, EAI, Haarlem, X, Beijing, Or- phan and 

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[HTML] CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING ISOLATES FROM THE MEDITERRANEAN AREA

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M Alonso, NA Rodriguez, C Garzelli… – BMC …, 2010 – biomedcentral.com

 deletion of the spacers 1-34 and the characteristic hybridization pattern of spacers 35-43, and
the remaining seven corresponded to variant “Beijing-like” spoligotypes Spoligotyping was
performed following the manufacturer’s instructions (Isogen, Netherlands). 

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SPOLIGOTYPE-BASED COMPARATIVE POPULATION STRUCTURE ANALYSIS OF MULTIDRUG-RESISTANT AND ISONIAZID-MONORESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES …

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T Jagielski, E Augustynowicz-Kopeć… – Journal of clinical …, 2010 – Am Soc Microbiol

 CTAB) method (47) were stored at −20°C before being subjected to spoligotyping using a 
hybridized to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science
BV  Spoligotypes in binary format were converted to an octal code for comparison with the 

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[HTML] FIRST INSIGHTS INTO THE GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM HIV-INFECTED MEXICAN PATIENTS AND MUTATIONS CAUSING MULTIDRUG RESISTANCE

[HTML] from biomedcentral.com

R Lopez-Alvarez, C Badillo-Lopez… – BMC …, 2010 – biomedcentral.com

 To determine lineage, the 57 strains (48 MTb and 9 M. bovis) from the MTC were spoligotyped
and binary outcomes were compared with the shared type (ST) number and lineages and
sublineages reported by  Spoligotyping Spoligotypes were reported using an octal code [74]. 

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MOLECULAR EVIDENCE OF FALSE-POSITIVE CULTURES FOR MYCOBACTERIUM TUBERCULOSIS IN A TAIWANESE HOSPITAL WITH A HIGH INCIDENCE OF TB

[HTML] from chestpubs.org

CC Lai, CK Tan, SH Lin, CH Liao… – Chest, 2010 – chestjournal.chestpubs.org

 Spoligotyping was performed according to the internationally standardized protocol to identify
and differentiate  10 A commercially available kit (Isogen Bioscience BV; Maarssen, The
Netherlands) was  database was used to assign genotype families to the spoligotypes obtained 

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MOLECULAR DETECTION OF MIXED INFECTIONS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN SPUTUM SAMPLES FROM PATIENTS IN KARONGA DISTRICT, MALAWI

[HTML] from nih.gov

K Mallard, R McNerney, AC Crampin… – Journal of clinical …, 2010 – Am Soc Microbiol

 on these isolates with the aid of a commercially available kit (Isogen Biosciences BV  These isolates
were also spoligotyped and had previously been characterized by IS6110-RFLP  Spoligotyping
versus Beijing and LAM PCR results for 377 clinical M. tuberculosis isolates used 

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[HTML] CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS CASES IN ACAPULCO: SPOLIGOTYPING AND RISK FACTORS

[HTML] from hindawi.com

E Nava-Aguilera, E Harris, S Mitchell… – Clinical and …, 2010 – hindawi.com

 DNA with 43 oligonucleotides covalently linked to a nylon membrane (Isogen Bioscience BV 
SpolTools permitted the analysis of M. tuberculosis spoligotyping data to identify emerging  and
visualization of the probable evolutionary relationships between the spoligotypes in our 

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TRACING THE ORIGINS OF< I> MYCOBACTERIUM BOVIS</I> TUBERCULOSIS IN HUMANS IN THE USA TO CATTLE IN MEXICO USING SPOLIGOTYPING

[HTML] from nih.gov

TC Rodwell, AJ Kapasi, M Moore… – International Journal of …, 2010 – Elsevier

 For this study, we also examined all available spoligotypes in the US national surveillance
database of M. bovis isolates sent to the National Veterinary Services Laboratories  Spoligotyping
was performed by all laboratories using the Spoligo Kit (Isogen Bioscience BV 

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HIGH SPOLIGOTYPE DIVERSITY WITHIN A< I> MYCOBACTERIUM BOVIS</I> POPULATION: CLUES TO UNDERSTANDING THE DEMOGRAPHY OF THE PATHOGEN IN EUROPE

[PDF] from tcd.ie

S Rodríguez, B Romero, J Bezos, L De Juan… – Veterinary …, 2010 – Elsevier

 The isolates were spoligotyped following the protocol described by Kamerbeek et al  Frequencies
of the 43 spacers of the DR locus that are included in the spoligotyping membrane (designed 
Data expressed as percentage of spoligotypes (n = 252) in which the spacer is present 

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MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE IS AN INDEPENDENT RISK FACTOR FOR TUBERCULOSIS TREATMENT FAILURE IN INDONESIA

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I Parwati, B Alisjahbana, L Apriani… – Journal of Infectious …, 2010 – jid.oxfordjournals.org

 of the Beijing genotype per se or to confounding by drug resistance, which has been linked to
the Beijing genotype in different settings [1]. We previously spoligotyped a large  Spoligotyping
was performed using a commercial kit (Isogen Bioscience) according to the 

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EFFECT OF SERIAL SUBCULTURING ON THE GENETIC COMPOSITION AND CYTOTOXIC ACTIVITY OF MYCOBACTERIUM TUBERCULOSIS

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CA Molina-Torres, J Castro-Garza… – Journal of medical …, 2010 – Soc General Microbiol

 (1997) was used. PCR amplification of the direct repeat locus was performed on heat-treated
cell suspensions. Biotin-labelled PCR products were detected by hybridization onto a
spoligotyping membrane (Isogen Bioscience). Microarray analysis. 

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[HTML] DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS GENOTYPES CIRCULATING IN NDOLA, ZAMBIA

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C Mulenga, I Shamputa, D Mwakazanga… – BMC infectious …, 2010 – biomedcentral.com

 Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according  Spoligotyping and MIRU-VNTR patterns were compared to the
international Spol DB4.0  Spoligotypes that were not present in the Spol DB4.0 are referred to 

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PHENOTYPIC AND GENOTYPIC ANALYSIS OF MULTIDRUG-RESISTANT TUBERCULOSIS IN ETHIOPIA

M Agonafir, E Lemma… – … of Tuberculosis and …, 2010 – ingentaconnect.com

 to the extraction protocol described by van Soolingen et al.14 Spoligotyping was performed  the
commercially available kit from Isogen Bioscience BV, Maarssen, The Netherlands, according
to  Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 

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[PDF] SENSIBILIDAD Y ESPECIFICIDAD DE PCR ANIDADA Y SPOLIGOTYPING COMO PRUEBAS RÁPIDAS DE DIAGNÓSTICO DE TUBERCULOSIS BOVINA EN TEJIDO FRESCO

[PDF] from scielo.org.mx

FM Suazoa, B Harrisb, CA Díazc… – Revista Mexicana de …, 2010 – scielo.org.mx

 Spoligotyping was performed essentially as described by Kamerbeek(17). Briefly,
amplification of DNA was run in  for 30 sec. Hybridization was performed as
recommended by the manufacturer (Isogen). Hybridized DNA was detected 

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CLINICAL AND GENOTYPIC CHARACTERISTICS OF EXTENSIVELY DRUG-RESISTANT AND MULTIDRUG-RESISTANT TUBERCULOSIS

CC Lai, CK Tan, SH Lin, CH Liao… – European journal of …, 2010 – Springer

 by serial dilution on agar plates as described previously [1]. Spoligotyping, according to  the M.
tuberculosis complex isolates [3]. A commercially available kit (Isogen Bioscience BV  1
Dendrograms of spoligotypes generated by clustered Myco- bacterium tuberculosis strains after 

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IDENTIFICATION OF WILD-TYPE MYCOBACTERIUM TUBERCULOSIS ISOLATES AND POINT MUTATIONS ASSOCIATED WITH ISONIAZID RESISTANCE

[PDF] from iekvm.kharkov.ua

OY Limanskaya, TN Mukhina, VN Stepanshina… – Molecular Biology, 2010 – Springer

 carrying AGC AGA; HB125, carrying AGC ACC, and H37Rv with the wild type codon
315 (negative control for amplification). The reaction mixture (20–50 μl) was prepared
from a dry amplification kit (Isogen, Russia). Its com 

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A SNAPSHOT OF THE BIODIVERSITY AND CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS IN OMAN USINGSPOLIGOTYPING

[DOC] from kit.nl

A Al-Maniri, JPN Singh, O Al-Rawas… – … of Tuberculosis and …, 2010 – ingentaconnect.com

 Figure Spoligotyped distribution of  Molecular typing of Mycobacterium tuberculosis isolates
from a rural area of Kanpur by spoligotyping and mycobacterial interspersed re- petitive units
(MIRUs) typing.  Predominant tubercu- losis spoligotypes, Delhi, India. 

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MYCOBACTERIUM TUBERCULOSIS COMPLEX CRISPR GENOTYPING: IMPROVING EFFICIENCY, THROUGHPUT AND DISCRIMINATIVE POWER OF ‘SPOLIGOTYPING’WITH NEW SPACERS AND A …

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J Zhang, E Abadia, G Refregier… – Journal of medical …, 2010 – Soc General Microbiol

 Thus, as spoligotypes are considered both as phylogenetic and epidemiological markers (Asiimwe
et al., 2008; Sola et al., 2001; Tanaka & Francis, 2006  Both sets had been previously genotyped
by spoligotyping using commercial membranes (Isogen Biosciences). 

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[HTML] MOLECULAR DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PATIENTS WITH TUBERCULOSIS IN HONDURAS

[HTML] from biomedcentral.com

S Rosales, L Pineda-García… – BMC …, 2010 – biomedcentral.com

 All isolates were spoligotyped in order to identify the predominant genotypes within this  SG
performed and analyzed RFLP; carried out bioinformatics analysis of spoligotyping and RFLP
results  NR performed database analysis of the spoligotypes and helped draft the manuscript 

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[HTML] PRODUCTION OF TNF-Α, IL-12 (P40) AND IL-17 CAN DISCRIMINATE BETWEEN ACTIVE TB DISEASE AND LATENT INFECTION IN A WEST AFRICAN COHORT

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JS Sutherland, BC de Jong, DJ Jeffries, IM Adetifa… – PloS one, 2010 – dx.plos.org

 10ng of DNA was used for spoligotype analysis with commercially available membranes (Isogen
Biosciences, The Netherlands).  Contributed reagents/materials/analysis tools: BCdJ. Wrote the
paper: JSS MOCO. Performed spoligotyping: BCdJ. Statistical analysis: DJJ. 

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[BOOK] RAPID TESTS FOR MULTIDRUG RESISTANT TUBERCULOSIS IN LOW INCOME SETTINGS

[PDF] from mak.ac.ug

F Bwanga – 2010 – mbl.mak.ac.ug

 In paper V, we applied spoligotyping to study the clustering rate and predominant genotypic strains
of 99 MDR-TB strains isolated from patients in Kampala. Eighty three percent of the strains
occurred in clusters, and the T2 lineage was the largest single cluster. Conclusion. 

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*************************************************************

2009

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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex

JR Driscoll – Methods Mol. Biol, 2009 – Springer

 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV  potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26).  spacer
sequences would improve the differentiability of the commonly observed spoligotypes

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Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol

 The fast DNA extraction method (amount used, 2 μl) was also successfully employed for
spoligotyping (Ocimum Biosolution Company, India).  In addition, further CAS MIRU-VNTR types
and associated spoligotypes were identified in the literature (3, 6, 7, 58, 61, 62). 

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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES (UAE)

[DOC] from qmul.ac.uk

A Saaidi… – 2009 – qmro.qmul.ac.uk

 HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 

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BEIJING GENOTYPE OF MYCOBACTERIUM TUBERCULOSIS IS SIGNIFICANTLY ASSOCIATED WITH HIGH-LEVEL FLUOROQUINOLONE RESISTANCE IN VIETNAM

[HTML] from nih.gov

DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol

 with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV  were assigned to the Euro-American clade on the basis of spoligotyping patterns 
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive 

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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

[PDF] from lrsitbrd.nic.in

TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in

Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 

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[HTML] SUPERVISED LEARNING FOR THE AUTOMATED TRANSCRIPTION OF SPACER CLASSIFICATION FROM SPOLIGOTYPE FILMS

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D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com

 a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen  Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a  AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of 

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POLYMORPHIC EXACT TANDEM REPEAT A (PETRA): A NEWLY DEFINED LINEAGE OF MYCOBACTERIUM TUBERCULOSIS IN ISRAEL ORIGINATING PREDOMINANTLY IN SUB-SAHARAN AFRICA

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol

 In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in 
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9  performed using a kit according to the manufacturer’s instructions (Isogen/Omicium 

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A RAPID AND SIMPLE METHOD FOR IDENTIFYING MYCOBACTERIUM TUBERCULOSIS W-BEIJING STRAINS BASED ON DETECTION OF A UNIQUE MUTATION IN RV0927C BY PCR-SSCP

X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier

 Spoligotyping was performed as previously described [16  in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for 

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Using a multiplex polymerase chain reaction for the identification of Beijing strains of Mycobacterium tuberculosis

JR Sun, SY Lee, HY Dou… – European Journal of Clinical …, 2009 – Springer

 Page 2. in the Isogen Bioscience spoligotyping kit (Isogen Life Science, the Netherlands).  Among
the 323 clinical MTB isolates, a total of 78 different spoligotypes were found. One hundred and
sixty-nine isolates were determined to belong to the Beijing family. 

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[HTML] Mycobacterium bovis en Argentina: aislamientos de gatos tipificados por spoligotyping

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MJ Zumárraga, M Martínez Vivot… – Revista argentina de …, 2009 – SciELO Argentina

 (8) , and using the spoligotyping kit (Isogen Biosolutions BV  Each of the different spoligotypes
were allocated a number.  H37Rv (ATCC 27294) and M. bovis Bacillus Calmette-Guerin (BCG)
(ATCC 27289) were included as reference strains in each spoligotyping experiment. 

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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex

JR Driscoll – Methods Mol. Biol, 2009 – Springer

 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV  potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26).  spacer
sequences would improve the differentiability of the commonly observed spoligotypes

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Use of spoligotyping and large sequence polymorphisms to study the population structure of the Mycobacterium tuberculosis complex in a cohort study of consecutive …

[HTML] from nih.gov

BC De Jong, M Antonio, T Awine… – Journal of clinical …, 2009 – Am Soc Microbiol

 After spectrophotometry, we used 10 ng of DNA for spoligotype analysis (16) with commercially
available membranes (Isogen Biosciences, Maarssen, The Netherlands  If spoligotype analysis
did not result in a clear pattern of spacers, the isolate underwent repeat spoligotyping

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Isoniazid and rifampicin resistance-associated mutations in Mycobacterium tuberculosis isolates from Yangon, Myanmar: implications for rapid molecular testing

[HTML] from oxfordjournals.org

H Valvatne, H Syre, M Kross… – Journal of …, 2009 – Br Soc Antimicrob Chemo

 identification test (GenProbe Incorporated, San Diego, CA, USA) and by spoligotyping (Isogen
Bioscience BV  A total of 43 different spoligotypes were observed among the 96 isoniazid-resistant 
of the 63 isolates that could not be differentiated by spoligotyping produced diverse 

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… of the Mycobacterium tuberculosis complex in Albania obtained by multilocus variable-number tandem-repeat analysis and spoligotyping reveals the presence of …

[HTML] from nih.gov

S Tafaj, J Zhang, Y Hauck, C Pourcel… – Journal of clinical …, 2009 – Am Soc Microbiol

 ethambutol (2.0 μg/ml) (5). Identification at the species level was performed by phenotypic methods
and verified by a genotypic method on isolates with rare spoligotypes and/or  Spoligotyping was
performed using a commercial membrane (Isogen, The Netherlands 

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Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis

PICPI Cafrune, LGPLG Possuelo… – Canadian Journal …, 2009 – NRC Research Press

 was performed using a commercial kit according to the manufacturer’s instructions (Isogen
Biosciences BV  Table 1. Accuracy of spoligotyping results according to culture grading.  A total
of 44 different spoligotypes were identified; 112 isolates (83%) were included in 21 shared 

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Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variable-number tandem repeats for geographically optimized …

[HTML] from nih.gov

MB Boniotti, M Goria, D Loda… – Journal of clinical …, 2009 – Am Soc Microbiol

 repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV  into 64 different patterns, while the seven M. caprae spoligotypes were subdivided  The
discrimination provided by combination of spoligotyping and ETR methods allowed for a 

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Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates

HY Dou, JJ Lu, CW Lin, JR Chang, JR Sun… – Journal of microbiological …, 2009 – Elsevier

 Spoligotyping was carried out using a spoligotyping kit according to the manufacturer’s instructions
(Isogen Bioscience BV). Spoligotypes common to more than one strain were designated as shared
types (ST) and assigned a shared international type number (SIT) according to 

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[PDF] Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing

[PDF] from medind.nic.in

S Shenai, C Rodrigues, M Sadani, N Sukhadia… – Indian J Tuberc, 2009 – medind.nic.in

 All the PZA resistant isolates were further spoligotyped to identify M. bovis, if present.  Finger
printing – Spoligotyping All the 63 culture isolates were subjected to spoligotyping using a
commercially available Isogen kit, Netherlands as described by Kamerbeek et al.11 Briefly 

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Molecular analysis of< i> Mycobacterium tuberculosis</i> from tuberculous meningitis patients in Thailand

J Yorsangsukkamol, A Chaiprasert, T Prammananan… – Tuberculosis, 2009 – Elsevier

 2.4. SpoligotypingSpoligotyping was performed with a commercially available
kit (Isogen Bioscience BV, Maarsen, The Netherlands).  25 All obtained spoligotypes
were compared to SpolDB4 for analysis. 2.5. Data analysis. 

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Prevalence of ST26 among untreated smear-positive tuberculosis patients from Karachi indicating ongoing transmission

S Shakoor, M Tanveer, Y Rafiq… – Scandinavian …, 2009 – informahealthcare.com

 Five different resistance phenotypes were identified among the isolates spoligotyped: isolated
low-level isoniazid resistance (resistant to 0.2 µg/ml) in 1 isolate; high-level isoniazid resistance
(resistant to 1  Spoligotypes Spoligotyping grouped 66 isolates into 9 different clusters. 

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[PDF] Reinout van Crevel1, Ida Parwati2, Edhyana Sahiratmadja2, Sangkot Marzuki3, Tom HM Ottenhoff4, Mihai G. Netea1, Andre van der Ven1, Ronald H. Nelwan5, …

[PDF] from kun.nl

BA van der Meer… – Factors Underlying the Success … – repository.ubn.kun.nl

 min. Spoligotyping was performed using a commercial kit (Isogen Bioscience,
Maarssen, The Netherlands). The  min. Spoligotyping was performed using a
commercial kit (Isogen Bioscience, Maarssen, The Netherlands). The 

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Emergence of new forms of totally drug-resistant tuberculosis bacilli

[HTML] from chestpubs.org

AA Velayati, MR Masjedi, P Farnia… – Chest, 2009 – chestjournal.chestpubs.org

 by polymerase chain reaction (PCR) using primers derived from the direct repeat sequence
(Isogen Bioscience; Maarssen, the  and CAS (n = 4; 17.5%) [Table 2]. The VNTR profiles of
spoligotypes strains were  Spoligotyping Patterns and VNTR Profiles of XDR and TDR Patients. 

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[PDF] Mycobacterium tuberculosis Beijing genotype in Indonesia is associated with drug resistance gene mutations in katG, rpoB and embB

[PDF] from kun.nl

I Parwati, R van Crevel, J de Beer… – Factors Underlying the … – repository.ubn.kun.nl

 of rpoB-hotspot region Three hundred and twenty five spoligotyped isolates were  Mycobacterium
tuberculosis complex genetic diversity: mining the fourth international spoligotyping database
(SpolDB4  mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug 

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Infection with Mycobacterium tuberculosis Beijing genotype strains is associated with polymorphisms in SLC11A1/NRAMP1 in Indonesian patients with tuberculosis

[HTML] from oxfordjournals.org

R Van Crevel, I Parwati… – Journal of Infectious …, 2009 – jid.oxfordjournals.org

 Mycobacterial DNA was extracted by bringing 2 loops of bacterial mass from a M.
tuberculosis culture in saline and subsequently heating it at 95°C for 5 min.
Spoligotyping was performed using a commercial kit (Isogen Bioscience). 

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Unexpectedly high proportion of ancestral Manu genotype Mycobacterium tuberculosis strains cultured from tuberculosis patients in Egypt

[HTML] from nih.gov

ZH Helal, MSED Ashour, SA Eissa… – Journal of clinical …, 2009 – Am Soc Microbiol

 was amplified with primers DRa and DRb, and the amplified biotinylated products were hybridized
to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science BV 
Comparison of spoligotyping results with database types.Spoligotypes in binary 

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Differences in Cell Wall Thickness between Resistant and Nonresistant Strains of< i> Mycobacterium tuberculosis</i>: Using Transmission Electron Microscopy

[PDF] from mrc.ac.ir

AA Velayati, P Farnia, TA Ibrahim… – …, 2009 – content.karger.com

 For spoligotyping, the direct repeat region was am- plified by PCR using primers derived from
the direct repeat se- quence (Isogen Bioscience).  Spoligopatterns and VNTR Profiles When the
spoligotype patterns were compared with earlier published spoligotypes [5] , our 

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Mycobacterium tuberculosis genotypic diversity in pyrazinamide-resistant isolates of Iran

[PDF] from mrc.ac.ir

F Doustdar, AD Khosravi… – Microbial Drug …, 2009 – online.liebertpub.com

 Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarsen, The  Only
1 (5.0%) of the 20 PZA-monoresistant isolates had spoligotyping patterns characteristic  Therefore,
the CAS superfamily spoligotypes showed no important correlation with MDR-TB as 

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Epidemiology of Mycobacterium bovis infection in wild boar (Sus scrofa) from Portugal

[HTML] from jwildlifedis.org

N Santos, M Correia-Neves… – Journal of wildlife …, 2009 – Wildlife Dis Assoc

 signatures were compared with those available in SpolDB4.0, the international spoligotyping
database (Brudey et al  The spoligotypes of 14 M. bovis isolates obtained from study areas B and
 originated from study area B. Isolates from the study area C were not spoligotyped

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Avaliação da técnica de spoligotyping aplicada diretamente em amostras clínicas de pacientes com suspeita de tuberculose e caracterização epidemiológica

[PDF] from ufrgs.br

PI Cafrune – 2009 – lume.ufrgs.br

 Spoligotyping technique presented good results when applied directly to clinical samples, once
89% of samples presented agreeing results. Among 135 patients isolates genotyped, it was found
44 differnt spoligotypes, 112 (83%) were shared types and 23(17%) were unique. 

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Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda

BB Asiimwe, J Asiimwe, G Kallenius… – Veterinary …, 2009 – veterinaryrecord.bmj.com

 The isolates were spoligotyped using the DNA extracted at the identification stage.  The
spoligotypes were analysed by BioNumerics software, version 5.0 (Applied Maths) as character 
were compared with those in SpolDB4.0, the international spoligotyping database (Brudey 

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High resolution typing system for pathogenic Mycobacterium tuberculosum

PS Keim, RS Spurgiesz… – US Patent 7,592,135, 2009 – Google Patents

 Spoligotyping is currently the most widely used PCR- based method of M. tuberculosum
genotyping due to the 65 relative technical ease of  In a database of 3,319 spoligotypes from
isolates collected in geographically distinct areas, seven spoligotype patterns were found for 

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Frequency of Mycobacterium bovis as an etiologic agent in extrapulmonary tuberculosis in HIV-positive and-negative Mexican patients

R Cicero, H Olivera, A Hernández-Solis… – European journal of …, 2009 – Springer

 a commercially available kit in accordance with the manufacturer’s recommendations (Isogen
Bioscience BV  and consistent identification of M. bovis was obtained by spoligotyping, showing
 cattle, where even isolates from the same herd show different spoligotypes [26] and 

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[HTML] In-depth molecular characterization of Mycobacterium tuberculosis from New Delhi–predominance of drug resistant isolates of the ‘modern'(TbD1−) type

[HTML] from plos.org

R Stavrum, VP Myneedu, VK Arora, N Ahmed… – PloS one, 2009 – dx.plos.org

 C membranes with the 43 synthetic oligonucleotides covalently bound to the membranes (Isogen
Bioscience BV  (2004) Predominant tuberculosis spoligotypes, Delhi, India.  repetitive-
unit-variable-number tandem-repeat typing system combined with spoligotyping for population 

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Predominance of Mycobacterium tuberculosis EAI and Beijing lineages in Yangon, Myanmar

[HTML] from nih.gov

S Phyu, R Stavrum, T Lwin… – Journal of clinical …, 2009 – Am Soc Microbiol

 (35). Spoligotyping was performed with commercially available activated Biodyne C membranes
that had 43 synthetic oligonucleotides covalently bound to the membranes (Isogen Bioscience
BV  Prevalence of spoligotypes in Yangon.The spoligotyping results obtained 

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[HTML] Tokyo-172 BCG vaccination complications, Taiwan

[HTML] from nih.gov

R Jou, WL Huang… – Emerging Infectious Diseases, 2009 – ncbi.nlm.nih.gov

 BCG, DNA samples were initially screened using a GenoType kit (Hain Lifescience GmbH, Nehren,
Germany), multiplex PCR (7), and pncA sequencing (8). In addition, spoligotyping was performed
with a commercial kit (Isogen Bioscience BV, Maarssen, the Netherlands). 

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Immunologic analysis of HIV-uninfected taiwanese children with BCG-induced disease

WI Lee, FC Liang, JL Huang, TH Jaing… – Journal of clinical …, 2009 – Springer

 also used to identify and differentiate M. tuberculosis complex isolates in this study [38], using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). The resolved spoligotypes were M. tuberculosis complex strain specific. The spoligotyping profile of 

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A rapid and simple method for identifying Mycobacterium tuberculosis W-Beijing strains based on detection of a unique mutation in Rv0927c by PCR-SSCP

X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier

 Spoligotyping was performed as previously described [16  in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for 

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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

[PDF] from lrsitbrd.nic.in

TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in

Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 

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Beijing genotype of Mycobacterium tuberculosis is significantly associated with high-level fluoroquinolone resistance in Vietnam

[HTML] from nih.gov

DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol

 with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV  were assigned to the Euro-American clade on the basis of spoligotyping patterns 
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive 

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Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol

 In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in 
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9  performed using a kit according to the manufacturer’s instructions (Isogen/Omicium 

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[HTML] Supervised learning for the automated transcription of spacer classification from spoligotype films

[HTML] from biomedcentral.com

D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com

 a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen  Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a  AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of 

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Potential challenges to the Stop TB Plan for humans in China; cattle maintain M. bovis and M. tuberculosis

[PDF] from yimg.com

Y Chen, Y Chao, Q Deng, T Liu, J Xiang, J Chen… – Tuberculosis, 2009 – Elsevier

 Spoligotyping was performed to define the genotype of Beijing family strains with a
commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s instruction. The spoligotype that only contained 

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[PDF] Tuberculosis Control in Oman

[PDF] from ki.se

A Al-Maniri – 2009 – diss.kib.ki.se

 Spoligotyping of 312 isolates of Mycobacterium tuberculosis from TB patients
diagnosed during 2005-2007 was carried out in study IV.  25 6.5 Study IV: Biodiversity
and clustering of M.tuberculosis isolates using spoligotyping ….. 

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[PDF] Molekularbiologischer Nachweis des Mycobacterium tuberculosis-Komplexes in altägyptischen Mumien unter besonderer Berücksichtigung neuer …

[PDF] from uni-muenchen.de

N Motamedi – 2009 – edoc.ub.uni-muenchen.de

 modernen und der ursprünglichen Form von M. tuberculosis. Van Embden et al. 68
untersuchten M. canetti mit einem erweiterten Spoligotyping (siehe unten), das mehr als
die 43 Spacer der etablierten Technik aufsucht. Hier fielen Spacer auf, 

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[HTML] Tuberculosis disparity between US-born blacks and whites, Houston, Texas, USA

[HTML] from nih.gov

JA Serpa, LD Teeter, JM Musser… – Emerging infectious …, 2009 – ncbi.nlm.nih.gov

 isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic  the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV 

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Molecular characterization of Mycobacterium Bovis isolates from selected slaughter houses in Kampala

[PDF] from mak.ac.ug

J Asiimwe – 2009 – dspace.mak.ac.ug

 Spoligotyping revealed that three of the M. bovis isolates had spoligopatterns that had previously
been reported in Uganda while the seven  Although a few clusters were identified on analysis
of the spoligotypes and RFLP patterns, no geographical sub-structuring was observed 

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Predictors of poor treatment outcome in multi-and extensively drug-resistant pulmonary TB

[HTML] from ersj.org.uk

K Kliiman… – European Respiratory Journal, 2009 – ersj.org.uk

 The strains were spoligotyped as described by Kamerbeek et al. 17 using commercially available
membranes (Isogen, Maarssen, The Netherlands) 17.  Mycobacterium tuberculosis complex genetic
diversity: mining the fourth international spoligotyping database (SpolDB4) for 

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[HTML] Tuberculosis Disparity between US-born Blacks and Whites, Houston, Texas, USA

[HTML] from cdc.gov

F Figures, I Capitalization, AS Guides… – Tuberculosis, 2009 – wwwnc.cdc.gov

 isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic  the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV 

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Molecular epidemiology of Mycobacterium tuberculosis in the United Arab Emirates (UAE)

[DOC] from qmul.ac.uk

A Saaidi… – 2009 – qmro.qmul.ac.uk

 HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 

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[PDF] EPIDEMIOLOGÍA MOLECULAR DE LA

[PDF] from ugr.es

TEN ALMERÍA – hera.ugr.es

Page 1. FACULTAD DE MEDICINA UNIVERSIDAD DE GRANADA DEPARTAMENTO
DE MEDICINA TÍTULO TESIS DOCTORAL: EPIDEMIOLOGÍA MOLECULAR DE LA
TUBERCULOSIS EN ALMERÍA Page 2. Editor: Editorial 

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2008

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Spoligotypes of< i> Mycobacterium tuberculosis</i> isolates of a high tuberculosis burden aboriginal township in Taiwan

PC Chuang, H Liu, C Sola, YMA Chen… – Infection, Genetics and …, 2008 – Elsevier

 A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as  All 138 isolates were successfully genotyped by spoligotyping, but only 122 were
of  A total of 27 spoligotypes were identified; 124 isolates (89.9%, 124/138) were 

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Genetic diversity of isoniazid-resistant Mycobacterium tuberculosis isolates collected in Poland and assessed by spoligotyping

[HTML] from nih.gov

E Augustynowicz-Kopeć, T Jagielski… – Journal of clinical …, 2008 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands), according to the manufacturer’s instructions and as  All spoligotypes obtained
were compared to those in the world spoligotyping database (SpolDB4) at 

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[HTML] Mycobacterium tuberculosis spoligotypes and drug susceptibility pattern of isolates from tuberculosis patients in peri-urban Kampala, Uganda

[HTML] from biomedcentral.com

B Asiimwe, S Ghebremichael… – BMC infectious …, 2008 – biomedcentral.com

 The obtained signatures were compared in binary format with the international spoligotyping
database of the Pasteur Institute of  Spoligotypes. To determine the strain lineages present in the
sample, the 344 isolates were spoligotyped and binary outcomes compared with those 

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Molecular typing of Mycobacterium tuberculosis isolates from a rural area of Kanpur by spoligotypingand mycobacterial interspersed repetitive units (MIRUs) typing

P Sharma, DS Chauhan, P Upadhyay… – Infection, Genetics and …, 2008 – Elsevier

 2.3. SpoligotypingSpoligotyping to detect 43 spacers was done with the help of commercially
available kit (Isogen Biosciences, BV, Maarsen, The Netherlands).  3.1. Spoligotyping. A total of
46 distinct spoligotypes were obtained from this set of 97 M. tuberculosis isolates. 

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Role of spoligotyping and IS6110-RFLP in assessing genetic diversity of< i> Mycobacterium tuberculosis</i> in India

JP Mathuria, P Sharma, P Prakash… – Infection, Genetics and …, 2008 – Elsevier

 spacers by using the standard method (Kamerbeek et al., 1997) with the help of commercially
available kit (Isogen Biosciences, BV  Spoligotyping Presence of 19 orphan isolates indicates
the need of conducting multicentric studies to know all the prevalent spoligotypes in Indian 

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[PDF] Investigation of Cross-Contamination in a Mycobacterium tuberculosis Laboratory Using Epidemiological data and Spoligotyping

[PDF] from pjms.com.pk

F Al-Otaibi… – pjms.com.pk

 Spoligotyping was performed with a commer- cially available kit (Isogen Bioscience).6,7 RESULTS 
Culturing of phosphate buffer in MGIT tube was positive for MTB after seven-day inoculation. Ten
different spoligotypes were identified among the 47 MTB isolates. 

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Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation

[PDF] from cnrs.fr

B Romero, A Aranaz, Á Sandoval, J Álvarez… – Veterinary …, 2008 – Elsevier

 detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV  The discriminatory capacity of spoligotyping was h = 0.38  The spoligotypes SB0950,
SB1230 and SB1232 were shared between domestic and wildlife animals (red 

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Spoligotyping of Mycobacterium tuberculosis in anthracotic bronchitis

K Najafizadeh, F Ghorbani, P Farnia… – … of Tuberculosis and …, 2008 – ingentaconnect.com

 use at −20°C. Spoligotyping Spoligotyping was performed, as previously described
by Kamerbeek et al.,10 with a commercially available kit (Isogen Bioscience BV,
Maarssen, The Netherlands). Analysis Information about the 

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Molecular analysis of drug‐resistant Mycobacterium tuberculosis isolates collected in central Poland

E Augustynowicz‐Kopeć, T Jagielski… – Clinical …, 2008 – Wiley Online Library

 DNA was prepared by the cetyl-trimethyl-ammonium bromide method [4]. Spoligotyping was
performed using a commercially available kit (Isogen Bioscience BV  was also performed as
described previously [2]. Isolates with indistinguishable spoligotypes and IS6110 

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[HTML] Human tuberculosis caused by Mycobacterium bovis, Taiwan

[HTML] from nih.gov

R Jou, WL Huang… – Emerging infectious diseases, 2008 – ncbi.nlm.nih.gov

 Currently, 119 M. bovis spoligotypes are contained in the fourth international spoligotyping
database (SpolDB 4) and  strains, presence or absence of region of difference 1 was analyzed
(5). Spoligotyping was performed with a commercial kit (Isogen Bioscience BV 

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A proposal for applying molecular markers as an aid to identifying potential cases of imported tuberculosis in immigrants

M Alonso, S Borrell, MM Lirola, E Bouza… – Tuberculosis, 2008 – Elsevier

 Spoligotyping was performed using primers of the spoligotyping kit (Isogen, Netherlands) and
following the manufacturer’s instructions. The spoligotypes were analyzed in the MIRU-VNTRplus
application (www.miru-vntrplus.org) 19 in order to identify the genetic lineages 

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Characterization of extensively drug-resistant Mycobacterium tuberculosis clinical isolates in China

[HTML] from nih.gov

Z Sun, Y Chao, X Zhang, J Zhang… – Journal of clinical …, 2008 – Am Soc Microbiol

 repeat (MIRU-VNTR) analysis. Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s protocols. PCR-based MIRU-VNTR analysis 

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[HTML] Incidence of tuberculosis and the predictive value of ELISPOT and Mantoux tests in Gambian case contacts

[HTML] from plos.org

PC Hill, DJ Jackson-Sillah, A Fox, RH Brookes… – PLoS One, 2008 – dx.plos.org

 For molecular subtyping of index case isolates, we extracted mycobacterial DNA using CTAB
and chloroform[18]. We performed spoligotyping using membranes from Isogen Biosciences
(http:/www.isogen-lifescience.com), as previously described[19]. 

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[PDF] : Transmission from South American Sea Lion () to Bactrian Camel () and Malayan Tapirs ()

[PDF] from cnrs.fr

I Moser, WM Prodinger, H Hotzel… – Veterinary …, 2008 – peer.ccsd.cnrs.fr

 by 30 cycles of 96°C for 15 sec, 67°C for 60 sec, 72°C for 60 sec, and a final extension at 18
72°C 300 sec. 19 SpoligotypingSpoligotyping (9; Isogen Bioscience, The Netherlands) was
performed using 20 DNA extracted from cultures. 21 MIRU typing. 

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Use of PCR-based Mycobacterium tuberculosis genotyping to prioritize tuberculosis outbreak control activities

[HTML] from nih.gov

M Ashworth, KL Horan, R Freeman… – Journal of clinical …, 2008 – Am Soc Microbiol

 Only one isolate per case was included in the analysis. Genotyping.Spoligotyping
was performed according to previously published methods by using a spoligotyping
kit (Isogen, IJsselstein, The Netherlands) (12). MIRU analysis 

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Mycobacteria causing human cervical lymphadenitis in pastoral communities in the Karamoja region of Uganda

[HTML] from nih.gov

J Oloya, J Opuda-Asibo, R Kazwala… – Epidemiology and …, 2008 – Cambridge Univ Press

 MTC were submitted for further differentiation by the spoligotyping kit ac- cording to the
manufacturer’s instructions (Isogen, Life Science  One of the spoligotypes (Hu-4 and Hu-5 in Fig.
1) is labelled LAM 3/S in the spoligotyping database SpolDB4, and has been isolated in 118 

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Genotypic diversity of extensively drug-resistant tuberculosis (XDR-TB) in South Africa

CK Mlambo, RM Warren, X Poswa… – … of tuberculosis and …, 2008 – ingentaconnect.com

 Spoligotyping was performed ac- cording to the internationally standardised protocol11 using
commercially  of the genotyping data, serial isolates from 13 patients were randomly sampled
and spoligotyped was used to assign geno- type families to the spoligotypes obtained.12 

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[HTML] Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei

[HTML] from biomedcentral.com

HY Dou, FC Tseng, CW Lin, JR Chang… – BMC infectious …, 2008 – biomedcentral.com

 Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not 
Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV  The resulting spoligotypes were documented under a binary code representing either a 

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[HTML] Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan

[HTML] from biomedcentral.com

M Tanveer, Z Hasan, A Siddiqui, A Ali… – BMC Infectious …, 2008 – biomedcentral.com

 Spoligotyping of M. tuberculosis isolates. All M. tuberculosis strains were spoligotyped and their
data analyzed using UPGMA calculation in the Bionumerics software.  one (78%) isolates grouped
into 59 different “shared types” while 205 (22%) isolates had ‘orphan’ spoligotypes

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< i> Mycobacterium pinnipedii</i>: Transmission from South American sea lion (< i> Otaria byronia</i>) to Bactrian camel (< i> Camelus bactrianus bactrianus</i>) and …

[PDF] from tcd.ie

I Moser, WM Prodinger, H Hotzel, R Greenwald… – Veterinary …, 2008 – Elsevier

 2.5. SpoligotypingSpoligotyping (Kamerbeek et al., 1997; Isogen Bioscience, The Netherlands)
was performed using DNA extracted from cultures. 2.6. MIRU typing. MIRU typing was
performed targeting 12 chromosomal loci as given by Mazars et al. 

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Mycobacterium tuberculosis population structures differ significantly on two Indonesian Islands

[HTML] from nih.gov

I Parwati, R Van Crevel, M Sudiro… – Journal of clinical …, 2008 – Am Soc Microbiol

 Therefore, we have spoligotyped M. tuberculosis isolates from a large cohort of patients 
Mycobacterium tuberculosis complex genetic diversity: mining the fourth international
spoligotyping database (SpolDB4) for  Global distribution of Mycobacterium tuberculosis 

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Development of multiplex assay for rapid characterization of Mycobacterium tuberculosis

[HTML] from nih.gov

IL Bergval, R Vijzelaar, ER Dalla Costa… – Journal of clinical …, 2008 – Am Soc Microbiol

 Strains 1 to 29 were selected for their specific spoligotypes and were used to determine the
correlation between MLPA, the spoligotype, and the sequence of ogt codon 15 (a marker for the
 Spoligotyping.Mycobacterium tuberculosis strains were spoligotyped by using a 

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Mycobacterium bovis in wildlife in France

[HTML] from jwildlifedis.org

G Zanella, B Durand, J Hars… – Journal of wildlife …, 2008 – Wildlife Dis Assoc

 Biotin-labeled PCR products were detected on a spoligotyping membrane (Isogen Bioscience BV,
Maarssen, The Netherlands). Purified sterile water and M. bovis and M. tuberculosis controls
included in the spoligomembrane kit were included in each batch of tests. 

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Distribution of rpoB mutations among multidrug‐resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation …

T Prammananan, W Cheunoy… – Clinical …, 2008 – Wiley Online Library

 In contrast, of 112 rifampicin-susceptible isolates that were spoligotyped, only 51 (45.5%) were
revealed to be  a One additional MDRTB isolate was not available for spoligotyping Association
of specific mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug 

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Molecular diversity of< i> Mycobacterium tuberculosis</i> isolates from patients with pulmonary tuberculosis in Sri Lanka

US Rajapaksa, TC Victor, AJ Perera… – Transactions of the …, 2008 – Elsevier

 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV, Maarssen,
The  Spoligotyping of 98 M. tuberculosis isolates revealed a total of 43 patterns, with six  The finding
of identical spoligotypes in geographically widely separated countries may be 

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[PDF] Mutations in rpoB gene and genotypes of rifampin resistant Mycobacterium tuberculosis isolates in Iran

[PDF] from nritld.ac.ir

F Doustdar, AD Khosravi, P Farnia, AR Bahrmand… – Tanaffos, 2008 – nritld.ac.ir

 tuberculosis isolates are defined on the basis of shared spoligotypes or closely related IS6110 
Spoligotyping was performed with a commercially available kit to detect 43 known spacers  DR
locus according to the instructions supplied by the manufacturer (Isogen Bioscience BV 

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Molecular epidemiology of Mycobacterium bovis: usefulness in international trade

[PDF] from usda.gov

F Milian-Suazo, B Harris, CA Díaz… – Preventive veterinary …, 2008 – Elsevier

 Spoligotyping was performed following the protocol described by Kamerbeek et al  Eighty-four
M. bovis strains from Mexico and the US were spoligotyped under the same protocol in two  When
comparing spoligotypes from the two laboratories, 50 (59%) out of the 84 strains were 

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Attempts to revive Mycobacterium tuberculosis from 300‐year‐old human mummies

E Lemma, O Zimhony, CL Greenblatt… – FEMS microbiology …, 2008 – Wiley Online Library

 Spoligotyping was performed at day 250 following the instructions of the manufacturer
of the ISOGEN Life Science Spoligotyping kit. Animal inoculations. Two sets of six
female guinea pigs weighing an average of 250 g were used. 

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Mycobacterium tuberculosis Uganda genotype is the predominant cause of TB in Kampala, Uganda

[PDF] from mak.ac.ug

BB Asiimwe, T Koivula, G Kallenius… – … of Tuberculosis and …, 2008 – ingentaconnect.com

 Data analysis Spoligotypes were analysed using BioNumerics soft- ware (version 4.6, Applied
Maths, Kortrijk, Belgium) as a character type.  Spoligotyping To determine the predominant strain
lineages, the 344 isolates were spoligotyped and binary outcomes compared with 

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Pyrazinamide resistance among South African multidrug-resistant Mycobacterium tuberculosis isolates

[HTML] from nih.gov

M Mphahlele, H Syre, H Valvatne… – Journal of clinical …, 2008 – Am Soc Microbiol

 (i) Spoligotyping.Spoligotyping was performed to further  The resulting amplification products were
then hybridized to a commercially available membrane (Isogen Bioscience BV, Bilthoven, The
Netherlands), with covalently linked parallel rows of 43 synthetic oligonucleotides 

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Genotyping North American animal Mycobacterium bovis isolates using multilocus variable number tandem repeat analysis

[HTML] from sagepub.com

LR Martinez, B Harris, WC Black… – Journal of Veterinary …, 2008 – vdi.sagepub.com

 The 6 isolates that comprised MLVA type N were divided into 3 spoligotypes: 1 isolate into SP2 
A larger database of MLVA typed isolates, in combination with spoligotyping and conventional
epidemiological investigations, can provide a  n. Spacer sequences, Isogen Life Science 

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[CITATION] First Insight Into Mycobacterium tuberculosis Biodiversity in Sri Lanka: Evidence for Predominance of Beijing Genotype and Ancestral Lineages Among …

US Rajapaksa, AJ Perera, TC Victor… – International Journal of …, 2008 – Elsevier

Import into BibTeX

[BOOK] Molecular characterization of Mycobacterium tuberculosis complex from Kampala, Uganda

[PDF] from mak.ac.ug

B Asiimwe – 2008 – dspace.mak.ac.ug

 and colleagues (69), which is commercially available as a kit (Isogen Bioscience BV  the important
lineages and sub-lineages in SpolDB4.0, the international spoligotyping database iii  To investigate
the association between the predominant spoligotypes, HIV sero- status and anti 

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[PDF] A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon

[PDF] from sote.hu

L Judit – phd.sote.hu

Page 1. A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon
Doktori értekezés Lukács Judit Semmelweis Egyetem Klinikai Orvostudományok
Doktori Iskola Témavezetı: Dr. Somoskövi Ákos, MTA doktora 

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IS6110 in oriC affects the morphology and growth of Mycobacterium tuberculosis and attenuates virulence in mice

Y Casart, L Turcios, I Florez, R Jaspe, E Guerrero… – Tuberculosis, 2008 – Elsevier

 DR locus was carried out as described earlier, 15 with membranes supplied by Isogen Bioscience
BV  to mention that all the clones isolated from 1C8 or 1D3 displayed identical spoligotyping and
MIRU  A1 insertion 12 (Figure 1B), (ii) The IS6110-RFLPs and spoligotypes did not 

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Intérêt du typage moléculaire pour l’épidémiologie de la tuberculose en Limousin (1998–2006)

[HTML] from em-consulte.com

H Bezanahary, MC Baclet, C Sola… – Médecine et Maladies …, 2008 – Elsevier

 Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping L’hybridation (1H à 60
°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV  sous forme d’un
code octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut 

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[PDF] TABLA 8.1 _ GEL DE CORRIMIENTO

[PDF] from ula.ve

TNN – Identificación y diagnóstico de Actinomicetales … – saber.ula.ve

 caontb. rivm. nl/Manuals/principl. html. Por su parte, Isogen Biosciences (http://www. isogen.
nl) ofrece membranas con ADN inmobilizado de espaciadores, para utilizarse en pruebas
de spoligotyping específicas para cepas de M. bovis/M. tuberculosis. 

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Diversidade genética de estirpes de mycobacterium tuberculosis em pacientes com tuberculose nos distritos de Buzi e Manhiça, em Moçambique

[PDF] from utl.pt

CLS Mucavele – 2008 – repository.utl.pt

 number of spoligotypes were identical to profiles found in the region. The data reported showed
that migration and HIV might be factors of dissemination of TB in Humans in Mozambique. Factors
that can compromise the performance of Spoligotyping technique and other 

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[BOOK] Molecular Characterisation of Antibiotic Resistance in Mycobacterium Tuberculosis

[PDF] from ki.se

P Juréen – 2008 – diss.kib.ki.se

 personnel (91). In order to overcome this, spoligotyping was evolved. This  repeat) (91).
MIRU-VNTR analyses copy-numbers of repeats, however, in contrast to spoligotyping,
these have several sites scattered around the genome. Since 

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2007

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Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis

[PDF] from mvlab-fudan.cn

J Chen, AG Tsolaki, X Shen, X Jiang, J Mei… – Tuberculosis, 2007 – Elsevier

 Spoligotyping was performed by using an Isogen Bioscience spoligotyping kit (Isogen Life Science 
Figure 3. Beijing/W Spoligotypes observed in 182 isolates with deletion RD105. Comparison
of the multiplex PCR with spoligotyping revealed 100% sensitivity and specificity for 

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Contribution of spoligotyping to the characterization of the population structure of Mycobacterium tuberculosis isolates in Portugal

[TXT] from min-saude.pt

S David, DR Ribeiro, A Antunes, C Portugal… – Infection, Genetics and …, 2007 – Elsevier

 Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV, Maarsen, Netherlands). The resulting spoligotypes were documented under a binary code
representing either a positive or negative hybridization result (n and o) and analyzed 

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Spoligotyping of< i> Mycobacterium tuberculosis</i> DNA from Archival Ziehl–Neelsen-stained sputum smears

N Suresh, J Arora, H Pant, T Rana… – Journal of microbiological …, 2007 – Elsevier

 available kit, according to the instructions supplied by the manufacturer (Isogen Bioscience, BV 
a positive signal in spoligotyping, thus demonstrating the efficiency of spoligotyping in a  analysis
of DNA extracted from stored ZN slides identified tuberculosis spoligotypes in 41 

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Improvement of spoligotyping with additional spacer sequences for characterization of Mycobacterium bovis and M. caprae isolates from Spain

MT Javed, A Aranaz, L De Juan, J Bezos… – Tuberculosis, 2007 – Elsevier

 using two membranes: a commercial membrane containing the 43 spacers described earlier
23 (Isogen Bioscience BV  Number of spoligotyping patterns of M. bovis and M. caprae.
Thirty-one different spoligotypes were identified among the 308 M. bovis (26 patterns) and 88 

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Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

[HTML] from sgmjournals.org

H Han, F Wang, Y Xiao, Y Ren… – Journal of medical …, 2007 – Soc General Microbiol

 To test the reproducibility of MIRU typing, we blindly typed 20 isolates in two independent
experiments. SpoligotypingSpoligotyping was performed with a commercial spoligotyping kit
(Isogen Bioscience), according to the instructions supplied by the manufacturer. 

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[PDF] … tuberculosis Beijingg family isolates from Hong Kong and comparisonn with 1S6110 restriction fragment lengthh polymorphism typing and spoligotyping

[PDF] from uva.nl

K Kremer, BKY Au, PCW Yip, R Skuce, P Supply… – dare.uva.nl

 with a commercial membranee containing the standard set of 43 spacer oligonucleotides (Isogen
Bio- sciencee BV  The usee of spacers 56, 57, 66, 67, and 68 in spoligotyping increased the  patterns
among the Beijing lineage strains in this study from three to five spoligotypes.. 

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Identification of Beijing lineage Mycobacterium tuberculosis with combined mycobacterial interspersed repetitive unit loci 26, 31, and ETR-A

[HTML] from nih.gov

PJ Chin, CC Chiu… – Journal of clinical microbiology, 2007 – Am Soc Microbiol

 These isolates were characterized by IS6110 RFLP following standard protocols (12) and
spoligotyping with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands)
(6). The characteristic  All other spoligotypes are defined as non-Beijing genotypes. 

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Znaczenie metody spoligotyping w epidemiologicznych dochodzeniach gruźlicy

[PDF] from viamedica.pl

E Augustynowicz-Kopeć… – Pneumonologia i …, 2007 – pneumonologia.viamedica.pl

 Autorzy dziękują Pani J. Scheenhart (Isogen Bioscience BV) za konsultację warunków repro 
IS6110 restriction fragment length polymor- phism patterns and spoligotypes determined by 
Spoligotyping of Myco- bacterium tuberculosis isolates from patients with pulmo- nary 

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[HTML] Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories

[HTML] from jpgmonline.com

C Rodrigues, D Almeida, S Shenai, N Goyal… – Indian Journal of …, 2007 – Medknow

 Spoligotyping Spoligotyping was performed with a commercially available kit (Isogen Bioscience). 
Remaining two isolates grown from smear positive specimens had different spoligotypes hence
were  Since spoligotyping is known to be less refractory for differentiation of M 

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Special Article-Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories

[PDF] from bioline.org.br

C Rodrigues, D Almeida, S Shenai… – Indian Journal of …, 2007 – bioline.org.br

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience).  Remaining
two isolates grown from smear positive specimens had different spoligotypes hence were  Since
spoligotyping is known to be less refractory for differentiation of M. tuberculosis, we 

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[HTML] Characterisation of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda

[HTML] from biomedcentral.com

J Oloya, R Kazwala, A Lund… – BMC …, 2007 – biomedcentral.com

 in mid-central and mid-western areas of Uganda and detected new and specific spoligotypes
not reported  Cultures belonging to the M. tuberculosis complex were submitted for further
differentiation by Spoligotyping [20] as described by the producer (Isogen, Life science 

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Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey

O Kisa, A Albay, O Baylan, E Tozkoparan… – Research in …, 2007 – Elsevier

 Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according to the  2.6. Comparison of spoligotypes with the international spoligotype
database. Spoligotyping results were entered in a binary format and octal code on Excel 

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Evolution of the extensively drug-resistant F15/LAM4/KZN strain of Mycobacterium tuberculosis in KwaZulu-Natal, South Africa

[HTML] from oxfordjournals.org

M Pillay… – Clinical infectious diseases, 2007 – cid.oxfordjournals.org

 SpoligotypingSpoligotyping was performed using a kit from Isogen-Life Science
in accordance with the manufacturer’s instructions. The presence or absence of 43
spacers in the direct-repeat region of isolates of M. tuberculosis 

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[HTML] Longitudinal assessment of an ELISPOT test for Mycobacterium tuberculosis infection

[HTML] from plos.org

PC Hill, RH Brookes, A Fox, D Jackson-Sillah… – PLoS medicine, 2007 – dx.plos.org

 We performed spoligotyping using membranes from Isogen Biosciences (http://www.isogen
lifescience.com), as previously described [18] and analysed the results with software that we
designed using Matlab software (MathWorks, http://www.mathworks.com). 

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[HTML] M. tuberculosis genotypic diversity and drug susceptibility pattern in HIV-infected and non-HIV-infected patients in northern Tanzania

[HTML] from biomedcentral.com

GS Kibiki, B Mulder, WMV Dolmans… – BMC …, 2007 – biomedcentral.com

 with samples which were INH-resistant, contained phenotypic rifampicin-resistant strains, or had
selected spoligotypes; phenotypic rifampicin  Spoligotyping 1 U/μl). Amplification and hybridization
were performed according to the manufacturer’s manual (Isogen Bioscience BV 

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Outbreak of Mycobacterium bovis infection in a wild animal park

SM Schmidbauer, P Wohlsein, G Kirpal… – Veterinary …, 2007 – veterinaryrecord.bmj.com

 and subtype of the MTC agent, 17 isolates from various organs were spoligotyped (Kamerbeek
and  FIG 2: Spoligotyping pattern of 17 mycobacterial isolates from various organs from seven 
in wild animals in Germany and in these cases the spoligotypes normally correspond to 

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Molecular investigation of recurrent tuberculosis in patients from Rwanda

AN Umubyeyi, IC Shamputa… – … of Tuberculosis and …, 2007 – ingentaconnect.com

 DNA fingerprinting techniques Spoligotyping was performed using a commercial kit (Isogen
Bioscience BV  4 of the 12 previously cured MDR-TB patients showed different spoligotypes,
suggesting re  of their recurrence (Figures 1 and 2). In contrast, the spoligotyping pat- terns of 

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[HTML] Using ELISPOT to expose false positive skin test conversion in tuberculosis contacts

[HTML] from plos.org

PC Hill, DJ Jeffries, RH Brookes, A Fox… – PLoS One, 2007 – dx.plos.org

 spectrophotometry. We performed Spoligotyping using membranes (Isogen
Biosciences), as previously described,[14] scanned the results and analysed them
with software designed in Matlab. Data management and analysis. 

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[PDF] Usefulness of molecular techniques to identify ongoing tuberculosis transmission in Saudi Arabia

[PDF] from smj.org.sa

SA Al-Hajoj, VK Mohammed… – Saudi medical journal, 2007 – smj.org.sa

 The labeled PCR product was used as a probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV, Maarssen, The
Netherlands). The 2 isolates were identical by both RFLP and spoligotyping methods Figure 

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Molecular clues of a microepidemy among homeless tuberculosis patients in Budapest due to a new and local Mycobacterium tuberculosis clade

C Ködmön, S Niemann, MC Gutierrez… – Infection, Genetics and …, 2007 – Elsevier

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands) according to the instructions of the manufacturer
(Kremer et al., 1999). Briefly, genomic mycobacterial DNA 

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Discovery of a novel Mycobacterium tuberculosis lineage that is a major cause of tuberculosis in Rio de Janeiro, Brazil

[HTML] from nih.gov

LCO Lazzarini, RC Huard, NL Boechat… – Journal of clinical …, 2007 – Am Soc Microbiol

 Spoligotyping.All M. tuberculosis strains were spoligotyped using a commercial kit (Isogen
Bioscience BV  as an octal code (9). The person who performed the spoligotyping was blinded
to  information on the shared-type distributions of M. tuberculosis spoligotypes worldwide (N 

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Genotype and phenotype relationships and transmission analysis of drug-resistant tuberculosis in Singapore

YJ Sun, ASG Lee, SY Wong… – … of Tuberculosis and …, 2007 – ingentaconnect.com

 Spoligotyping Spoligotyping was performed using a commercial kit obtained from Isogen
Bioscience BV  as previously described.21,22 IS6110 RFLP patterns and/or MIRU-VNTR patterns
were used to determine genotypes for a few isolates with indeterminate spoligotypes

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Evidence of ‘amplifier effect’in pulmonary multidrug-resistant tuberculosis: report of three cases

A Umubyeyi, IC Shamputa, L Rigouts… – International Journal of …, 2007 – Elsevier

 SpoligotypingSpoligotyping was performed with a commercial kit (Isogen Bioscience
BV, Maarssen, The Netherlands) by the previously described method. 10 Briefly, 4
μl of the heat-killed bacterial suspension from each sample 

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[PDF] STUDY OF ISONIAZID-RESISTANT TUBERCULOSIS: MYCOBACTERIAL GENOTYPIC CHARACTERIZATION AND CLINICAL FEATURES IN HUMAN

[PDF] from usc.edu

P Escalante – 2007 – digitallibrary.usc.edu

 BioImage Whole Band Analyzer™, version 3.2 software (BioImage, Ann Arbor, MI). Spoligotyping
was performed using the Isogen™ kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all
isolates according to the manufacturer instructions. In addition, all 

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[PDF] GENOTIPAGEM UTILIZANDO A SEQÜÊNCIA DE INSERÇÃO IS6110 E “SPOLIGOTYPING” DE Mycobacterium tuberculosis ISOLADOS DE PACIENTES …

[PDF] from sp.gov.br

AC PANUNTO – crt.saude.sp.gov.br

 35 Tabela 3- Perfis agrupados de IS6110-RFLP presentes no estudo….. 50
Tabela 4- Perfis agrupados de “Spoligotyping” presentes no estudo….. 53
Tabela 5- “Spoligotypes” mais freqüentes no estudo….. 54 

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[BOOK] Study of isoniazid-resistant tuberculosis: Mycobacterial genotypic characterization and clinical features in human subjects

P Escalante – 2007 – books.google.com

 software (BioImage, Ann Arbor, MI). Spoligotyping was performed using the Isogen
kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all isolates according to
the manufacturer instructions. In addition, all isolates also 

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First insight into the population structure of Mycobacterium tuberculosis in Saudi Arabia

[HTML] from nih.gov

SAM Al-Hajoj, T Zozio, F Al-Rabiah… – Journal of clinical …, 2007 – Am Soc Microbiol

 to inter-DR spacer oligonucleotides covalently bound to a membrane (Isogen Life Science  All
spoligotypes were converted into octal format within Excel spreadsheets (8) and  The individual
spoligotyping patterns were compared with an updated SpolDB4 database named 

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Transmission of< i> Mycobacterium tuberculosis</i> in a Family Proved by Genotyping

CY Yang, R Jou, PC Chuang, JT Chang… – Journal of the Formosan …, 2007 – Elsevier

 Spoligotyping Spoligotyping is a polymerase chain reaction (PCR)-based analysis that uses
a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands).  The re-
solved spoligotypes were M. tuberculosis complex strain-specific. 

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[PDF] Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey

[PDF] from microbiologica.net

C Bicmen, N Esen, EA Graviss… – … -Quarterly Journal of …, 2007 – microbiologica.net

 were analyzed with the BioImage (Ann Arbor, Mich.,USA) Whole Band Analyzer, version 3.4.2.
Spoligotyping was per- formed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) in accordance with the manufacturer’s recom- mendations. 

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Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis

[HTML] from nih.gov

P Farnia, MR Masjedi, B Nasiri… – Epidemiology and …, 2007 – Cambridge Univ Press

 [12] with a commercially avail- able kit (Isogen Bioscience BV  Stability of M. tuberculosis IS6110
restriction fragment length polymorphism patterns and spoligotypes determined by  expanding
clones of M. tuberculosis and their global distribution assessed by spoligotyping in an 

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Identification of unique genetic markers in Rv0927c among< i> Mycobacterium tuberculosis</i> W-Beijing strains

X Jiang, Y Zhang, W Zhang, F Gao, J Jin… – Microbes and …, 2007 – Elsevier

 SDS) at 60 °C and then incubated in 1:4000 diluted streptavidin–peroxidase conjugate (Isogen
Bioscience) for  This finding, which showed a spoligotyping pattern with hybridization only to the
spacer 35 to 43  1. Spoligotypes of the 56 M. tuberculosis clinical isolates from China. 

Cited by 8 – Related articles – All 5 versions – Import into BibTeX

Laboratory diagnosis of pulmonary tuberculosis in TB and HIV endemic settings and the contribution of real time PCR for M. tuberculosis in bronchoalveolar lavage …

[PDF] from ru.nl

GS Kibiki, B Mulder… – Tropical Medicine & …, 2007 – Wiley Online Library

 Spoligotyping patterns were analysed with Bionumerics 4.0 (Applied Maths, St-Martin-Latem,
Belgium).  strains in 25 of the 28 BAL samples with positive MTB culture could be spoligotyped.
In all 25, the spoligotypes indicated that the isolates belonged to M. tuberculosis complex 

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[PDF] MYCOBACTERIOSIS IN WILD BOAR: RESULTS OF 2000-2006 ACTIVITY IN NORTH-WESTERN ITALY

[PDF] from vet-alfort.fr

A Dondo, S Zoppi, F Rossi, L Chiavacci, A Barbaro… – aeema.vet-alfort.fr

 37 a PCR based reverse line blot method covalently (Isogen, Netherland). M. bovis strains isolated
were further characterized by VNTR typing (ETR A, B, C, D, E) according to Frothingham
[Frothingham et al., 1998]. The strains unidentified by Spoligotyping were processed by a 

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Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern Ukraine

VV Nikolayevskyy, TJ Brown… – Clinical …, 2007 – Wiley Online Library

 [23] using commercially available membranes (Isogen, Maarssen, The  binding to the
corresponding targets, but an association between these specific spoligotypes and mutations
in  Spoligotyping results demonstrated a relative diversity in the genetic structure of M. tuberculosis 

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Discrepant Results Between Pyrazinamide Susceptibility Testing by the Reference BACTEC 460TB Method and pncA DNA Sequencing in Patients Infected With …

J Dormandy, A Somoskovi, BN Kreiswirth… – Chest, 2007 – chestjournal.chestpubs.org

 Spoligotyping, a polymerase chain reaction-based genotyping method that detects 43 known
spacer sequences that are interspersed between the direct repeats in the genomic repeat region
of M tuberculosis, was performed with a commercially available kit (Isogen Bioscience 

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Új Mycobacterium tuberculosis törzs okozta mikroepidémia budapesti hajléktalanokban

C Ködmön, J Lukács, S Dávid, I Vadász… – Orvosi Hetilap, 2007 – akademiai.com

 A spoligotyping kevésbé érzékeny, mint az IS6110-RFLP fin- gerprint módszer, de kitûnôen
elkülöníthetôk vele a világszer- te  identifikáltuk [5, 6]. A mycobacteriális DNS kinyerését és a
spoligotipizálást ke- reskedelmi forgalomban kapható teszttel (Isogen Bioscience BV 

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Caracterização das mutações envolvidas na resistência de isolados de Mycobacterium tuberculosis à estreptomicina e sua relação com o sistema de efluxo

[PDF] from ufrgs.br

FS Spies – 2007 – lume.ufrgs.br

 48 3.18. Genotipagem dos isolados ….. 48 3.18.1.
Spoligotyping ….. 49 3.18.1.1. Primers….. 49 3.18.1.2. 

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[PDF] Estudo de fatores relacionados ao controle da tuberculose: resistência às drogas, transmissão e suscetibilidade do hospedeiro; Study of factors related to the …

[PDF] from fiocruz.br

MDB Fernandes – 2007 – bdtd.cict.fiocruz.br

 32 Figura 2.8: Representação esquemática do spoligotyping. 35 Figura 2.9: Descrição do
spoligotyping de linhagens das maiores famílias do Mycobacterium tuberculosis. 36 Figura 2.10:
Representação esquemática da seqüência replicada utilizando DRE-PCR. 

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[PDF] PROYECTO DE MODIFICACIÓN A LA NORMA OFICIAL MEXICANA NOM-031-ZOO-1995, CAMPAÑA NACIONAL CONTRA LA TUBERCULOSIS BOVINA ( …

[PDF] from inforural.com.mx

OIE de Salud Animal – inforural.com.mx

Page 1. 54 (Segunda Sección) DIARIO OFICIAL Lunes 3 de diciembre de 2007
SECRETARIA DE AGRICULTURA, GANADERIA, DESARROLLO RURAL, PESCA
Y ALIMENTACION PROYECTO de Modificación a la Norma 

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CARACTERIZAÇÃO DAS MUTAÇÕES ENVOLVIDAS NA RESISTÊNCIA DE ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS À ESTREPTOMICINA E SUA RELAÇÃO COM O SISTEMA DE EFLUXO

[PDF] from ufrgs.br

FS Spies – 2007 – lume.ufrgs.br

 48 3.18. Genotipagem dos isolados ….. 48 3.18.1.
Spoligotyping ….. 49 3.18.1.1. Primers….. 49 3.18.1.2. 

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[PDF] ESTUDO DE FATORES RELACIONADOS AO CONTROLE DA TUBERCULOSE: RESISTÊNCIA ÀS DROGAS, TRANSMISSÃO E SUSCETIBILIDADE DO HOSPEDEIRO; STUDY OF FACTORS RELATED TO THE …

[PDF] from fiocruz.br

MDB Fernandes – 2007 – bdtd.cict.fiocruz.br

 32 Figura 2.8: Representação esquemática do spoligotyping. 35 Figura 2.9: Descrição do
spoligotyping de linhagens das maiores famílias do Mycobacterium tuberculosis. 36 Figura 2.10:
Representação esquemática da seqüência replicada utilizando DRE-PCR. 

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*************************************************************

2006

************************************************************

A case of urinary tuberculosis due toMycobacterium bovissubspeciescaprae

V Sintchenko, P Jelfs, M Dally… – Pathology-Journal of …, 2006 – journals.lww.com

 Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the
MTBC direct repeat  of reference laboratories to differentiate members of the MTBC by spoligotyping,
11 based on  Several spoligotypes have been recognised among M. bovis subsp. 

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… tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping

[HTML] from nih.gov

C Allix, K Walravens, C Saegerman… – Journal of clinical …, 2006 – Am Soc Microbiol

 PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV  IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals  based
on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated 

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Spoligotyping of Mycobacterium tuberculosis isolates from Pakistan reveals predominance of Central Asian Strain 1 and Beijing isolates

[HTML] from nih.gov

Z Hasan, M Tanveer, A Kanji, Q Hasan… – Journal of clinical …, 2006 – Am Soc Microbiol

 positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig.
1). A total of 197 (63%) isolates were grouped into 22 different clusters, while
119 (37%) isolates had unique spoligotypes. Previously 

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Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the samespoligotyping profile as isolates from animals

[HTML] from nih.gov

B Romero, A Aranaz, L de Juan… – Journal of clinical …, 2006 – Am Soc Microbiol

 Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,
The  of techniques is required for appropriate discrimination, even for those spoligotypes that have
 Furthermore, the design of a new spoligotyping membrane that complements the 

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< i> rpoB</i> gene sequencing and spoligotyping of multidrug-resistant< i> Mycobacterium tuberculosis</i> isolates from India

N Suresh, UB Singh, J Arora, H Pant… – Infection, Genetics and …, 2006 – Elsevier

 to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to
identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2.  Spoligotyping.
A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. 

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Usefulness of restriction fragment length polymorphism and spoligotyping for epidemiological studies of< i> Mycobacterium bovis</i> in Madagascar: Description of …

V Rasolofo Razanamparany, R Quirin… – Veterinary …, 2006 – Elsevier

 containing 43 oligonucleotides derived from spacer DNA sequences (obtained from Isogen
Bioscience BV, Utrecht, The Netherlands). PCR and hybridization were performed as described
by Kamerbeek et al. (1997). Spoligotyping patterns or spoligotypes were confirmed by 

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[HTML] Variantes genéticas de Mycobacterium tuberculosis aisladas de pacientes de la Xª Región de Chile

[HTML] from scielo.cl

M Mancilla E, A Martínez H… – Revista chilena de …, 2006 – SciELO Chile

 A significant number of spoligotypes were identical to profiles found in Brazil followed by Argentina
and Spain.  utilizando los partidores biotinilados DRa (5′-GGTTTTGGGTCTGACGAC) y DRb (5′-
CCGAGA GGGGACGGAAAC), incluidos en el sistema de spoligotyping (ISOGEN

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Mycobacterium africanum elicits an attenuated T cell response to early secreted antigenic target, 6 kDa, in patients with tuberculosis and their household contacts

[HTML] from oxfordjournals.org

BC de Jong, PC Hill, RH Brookes… – Journal of Infectious …, 2006 – jid.oxfordjournals.org

 were assessed by spectrophotometry (Eppendorf BioPhotometer). Spoligotyping
was performed using membranes (Isogen Biosciences), in accordance with
standardized methods [19]. Spoligotyping results were scanned and 

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rpoB gene mutations and molecular characterization of rifampin-resistant Mycobacterium tuberculosis isolates from Shandong Province, China

[HTML] from nih.gov

X Ma, H Wang, Y Deng, Z Liu, Y Xu… – Journal of clinical …, 2006 – Am Soc Microbiol

 (20), with data analyzed by Whole Band Analysis software (version 3.2; BioImage,
Ann Arbor, MI). Spoligotyping was carried out by using commercial kits from Isogen
Bioscience BV (Maarssen, The Netherlands). Mycobacterial 

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[HTML] Tuberculosis associated with Mycobacterium tuberculosis Beijing and non-Beijing genotypes: a clinical and immunological comparison

[HTML] from biomedcentral.com

YJ Sun, TK Lim, A Ong, B Ho… – BMC infectious …, 2006 – biomedcentral.com

 Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, the
Netherlands  The remaining one isolate (N07) was not detected by spoligotyping, but MIRU-VNTR 
Based on spoligotypes and/or MIRU-VNTR patterns, the remaining 20 isolates were 

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Prevalence of Haarlem I and Beijing types of Mycobacterium tuberculosis strains in Iranian and Afghan MDR-TB patients

[PDF] from mrc.ac.ir

P Farnia, MR Masjedi, M Mirsaeidi, F Mohammadi… – Journal of Infection, 2006 – Elsevier

 SpoligotypingSpoligotyping was performed as described by Kamerbeek et al. 5 with a
commercially available kit, according to the instructions supplied by the manufacturer (Isogen
Bioscience BV, Maarsen, The Netherlands).  Distribution of the 263 MDR-TB spoligotypes

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Molecular epidemiology of drug-resistant tuberculosis in Hungary

[HTML] from nih.gov

C Ködmön, S Niemann, J Lukács… – Journal of clinical …, 2006 – Am Soc Microbiol

 described previously (3, 14, 17). Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
instructions of the manufacturer (10). The IS6110 fingerprint and 

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Tuberculosis in mandrills at the Lisbon zoo

A Amado, T Albuquerque, A Gonçalves… – Veterinary …, 2006 – veterinaryrecord.bmj.com

 In order to investigate the infection source, spoligotyping was performed using conventional 43
oligonucleotide Isogen membranes as described by Kamerbeek and others (1997). The two
isolates shared the same spoligotype described as type 237 (Anon 2006). 

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[HTML] Molecular characterisation of Mycobacterium tuberculosis isolates in the First National Survey of Anti-tuberculosis Drug Resistance from Venezuela

[HTML] from biomedcentral.com

L Aristimuño, R Armengol, A Cebollada… – BMC …, 2006 – biomedcentral.com

 to a set of 43 oligonucleotides covalently bound to the spoligo-membrane (Isogen Biosciences
BV  of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an
International study Global distribution of Mycobacterium tuberculosis spoligotypes

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[PDF] 2. Lipoarabinomannans—structurally diverse and functionally enigmatic macroamphiphiles of mycobacteria and related actinomycetes•

[PDF] from pasteur.ac.ir

I Sutcliffe, T Matsumoto, H Ano, T Nagai, K Danno… – 2006 – journals2005.pasteur.ac.ir

 Spoligotyping The clones of M. tuberculosis with less than five IS6110 RFLP bands were subjected
to the second typing, spoligotypingSpoligotyping membranes were purchased from Isogen
bioscience BV (Biltho- ven, The Netherlands). The procedures were 

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Beijing genotype of Mycobacterium tuberculosis is significantly associated with human immunodeficiency virus infection and multidrug resistance in cases of …

[HTML] from nih.gov

M Caws, G Thwaites, K Stepniewska… – Journal of clinical …, 2006 – Am Soc Microbiol

 Spoligotyping.Spoligotyping of isolates was performed according to the standard protocol (6).
Briefly, DNA  Dra (biotin labeled) and DRb, hybridized to the spoligotype membrane (Isogen
Bioscience BV  Spoligotypes were available for isolates from 35 HIV-positive patients (Fig. 

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The most prevalent Mycobacterium tuberculosis superfamilies among Iranian and Afghan TB cases

[PDF] from mrc.ac.ir

AA Velayati, P Farnia, M Mirsaeidi… – … journal of infectious …, 2006 – informahealthcare.com

 [7] with a commercially available kit, according to the instructions supplied by the manufacturer
(Isogen Bioscience BV, Maarsen, The Netherlands).  Spoligotyping  Figure 1. Shows the
predominant spoligotypes which are prevalent among Iranian and Afghan-born immigrants. 

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Extensively drug-resistant tuberculosis: 2 years of surveillance in Iran

[HTML] from oxfordjournals.org

MR Masjedi, P Farnia, S Sorooch… – Clinical infectious …, 2006 – cid.oxfordjournals.org

 [13] with a commercially available kit (Isogen Bioscience).  On the basis of spoligotyping results,
these strains were determined to belong to the East African Indian 3 M. tuberculosis superfamily.
Table 1. Table 1  Distribution of the 12 XDR TB spoligotypes

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[HTML] Predominance of ancestral lineages of Mycobacterium tuberculosis in India

[HTML] from 9med.net

MC Gutierrez, N Ahmed, E Willery… – Emerg Infect …, 2006 – journal.9med.net

 Spoligotyping was performed by using a commercial kit (Isogen Bioscience BV, Maarsen, the
Netherlands) according to  was built based on spoligotypes alone or on a combination of VNTR
and spoligotypes, although the resolution was lower when spoligotyping was used 

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High resolution typing system for pathogenic Mycobaterium tuberculosum

PS Keim, RS Spurgiesz, JM Schupp – US Patent 7,026,467, 2006 – Google Patents

 7:390-6. 2001. Sola, C. et al., “Mycobacterium tuberculosis phylogeny reconstruction based on
combined numerical analysis with IS1081, IS6110, VNTR, and DR-based spoligotyping sug-
gests the existence of two new phlyogeographical clades”. J Molecr Ev. 53:680-9. 2001. 

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Mycobacterium tuberculosis genotypes and their relationships with clinical and immunological phenotypes in Singapore

[PDF] from 137.132.14.55

SUN YONGJIANG – 2006 – 137.132.14.55

 Furthermore, this method is highly reproducible and easy to perform (Kremer et al., 1999);
particularly spoligotypes can be digitized into binary or octal code formats (Dale et al., 2001)
which greatly facilitate the management and interlaboratory comparison of spoligotyping data. 

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Recent transmission of tuberculosis involving retired patients

PI Cafrune, LW Riley, LG Possuelo, ARM Valim… – Journal of Infection, 2006 – Elsevier

 SpoligotypingSpoligotyping was performed as described by Kamerbeek et al. 19 
probed. Hybridization was performed with a commercial kit, according to the
manufacturer instructions (Isogen Biosciences BV, The Netherlands). 

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[PDF] INFORME FINAL DE PROYECTO DE INVESTIGACIÓN

[PDF] from ins.gob.pe

EN MULTIDROGORESISTENCIA… – bvs.ins.gob.pe

 Salud e interpretadas en el software ALFWin TM Sequence Analyser 2.0 1998. Ensayo
de genotipificación mediante Spoligotyping Fue usado un kit comercialmente
disponible (Isogen Bioscience BV, Maarssen, The Netherlands 

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Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis

[PDF] from vetres-archive.org

A Aranaz, L De Juan, J Bezos, J Álvarez… – Veterinary …, 2006 – vetres.org

 Thirty-six M. bovis isolates were char- acterised by spoligotyping and MIRU- VNTR: the 23 M  The
biotin-labelled amplified product was hybridised onto a membrane (Isogen Bio- science BV,
Maarssen  Four spoligotypes were found, and three of them, numbered spb-7, spb-9 

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Epidemiologia molecular da tuberculose resistente a múltiplos fármacos no Rio Grande do Sul

[PDF] from ufrgs.br

ARM Valim – 2006 – lume.ufrgs.br

 72 4.6.4 Hibridização e Detecção….. 73 4.7 Spoligotyping …..  84 5.6 Análise dos
Padrões Gerados pela Genotipagem por Spoligotyping ….. 92 4.7. 

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EPIDEMIOLOGIA MOLECULAR DA TUBERCULOSE RESISTENTE A MÚLTIPLOS FÁRMACOS NO RIO GRANDE DO SUL

[PDF] from ufrgs.br

ARM Valim – 2006 – lume.ufrgs.br

 72 4.6.4 Hibridização e Detecção….. 73 4.7 Spoligotyping …..  84 5.6 Análise dos
Padrões Gerados pela Genotipagem por Spoligotyping ….. 92 4.7. 

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INTÉRÊT DU TYPAGE MOLÉCULAIRE POUR L’ÉPIDÉMIOLOGIE DE LA TUBERCULOSE EN LIMOUSIN (1998–2006)

[HTML] from em-consulte.com

H Bezanahary, MC Baclet, C Sola… – Médecine et Maladies …, 2008 – Elsevier

 Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping L’hybridation (1H à 60
°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV  sous forme d’un
code octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut 

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… TANDEM-REPEAT GENOTYPING OF MYCOBACTERIUM BOVIS AND COMPARISON OF THE METHOD WITH IS6110 RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS AND SPOLIGOTYPING

[HTML] from nih.gov

C Allix, K Walravens, C Saegerman… – Journal of clinical …, 2006 – Am Soc Microbiol

 PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV  IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals  based
on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated 

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A CASE OF URINARY TUBERCULOSIS DUE TOMYCOBACTERIUM BOVISSUBSPECIESCAPRAE

V Sintchenko, P Jelfs, M Dally… – Pathology-Journal of …, 2006 – journals.lww.com

 Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the
MTBC direct repeat  of reference laboratories to differentiate members of the MTBC by spoligotyping,
11 based on  Several spoligotypes have been recognised among M. bovis subsp. 

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SPOLIGOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PAKISTAN REVEALS PREDOMINANCE OF CENTRAL ASIAN STRAIN 1 AND BEIJING ISOLATES

[HTML] from nih.gov

Z Hasan, M Tanveer, A Kanji, Q Hasan… – Journal of clinical …, 2006 – Am Soc Microbiol

 positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig.
1). A total of 197 (63%) isolates were grouped into 22 different clusters, while
119 (37%) isolates had unique spoligotypes. Previously 

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MOLECULAR EPIDEMIOLOGY OF MULTIDRUG-RESISTANT MYCOBACTERIUM BOVIS ISOLATES WITH THE SAMESPOLIGOTYPING PROFILE AS ISOLATES FROM ANIMALS

[HTML] from nih.gov

B Romero, A Aranaz, L de Juan… – Journal of clinical …, 2006 – Am Soc Microbiol

 Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,
The  of techniques is required for appropriate discrimination, even for those spoligotypes that have
 Furthermore, the design of a new spoligotyping membrane that complements the 

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< I> RPOB</I> GENE SEQUENCING AND SPOLIGOTYPING OF MULTIDRUG-RESISTANT< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM INDIA

N Suresh, UB Singh, J Arora, H Pant… – Infection, Genetics and …, 2006 – Elsevier

 to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to
identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2.  Spoligotyping.
A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. 

Cited by 22 – Related articles – All 7 versions – Import into BibTeX

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2005

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[HTML] Spoligotyping of Mycobacterium bovis isolates found in Manitoba

[HTML] from nih.gov

C Lutze-Wallace, C Turcotte… – Canadian journal of …, 2005 – ncbi.nlm.nih.gov

 Table I. Table I Summary of spoligotyped isolates by year, species, and location.  types are
656573377603600 (MB-1) and 656473377603600 (MB-2). These spoligotypes have not 
Spoligotyping is particularly useful for differentiating isolates harbouring only a single copy of 

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Characterization of Mycobacterium tuberculosis complex isolates from Greek patients with sarcoidosis by spoligotyping

[HTML] from nih.gov

M Gazouli, J Ikonomopoulos… – Journal of clinical …, 2005 – Am Soc Microbiol

 hybridization with membrane-bound spacer oligonucleotides and a spoligotyping kit (ISOGEN
Bioscience, Maarsen  The spoligotyping results were entered in a binary format as Excel
spreadsheets  All of the identified spoligotypes have been submitted to the World Spoligotype 

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… Mycobacterium tuberculosis Beijing family isolates from Hong Kong and comparison with IS6110 restriction fragment length polymorphism typing and spoligotyping

[HTML] from nih.gov

K Kremer, BKY Au, PCW Yip, R Skuce… – Journal of clinical …, 2005 – Am Soc Microbiol

 In addition to spoligotyping with a commercial membrane containing the standard set of 43 spacer
oligonucleotides (Isogen Bioscience BV, Maarssen, The  (35), who also subjected Beijing strains
to spoligotyping with novel spacers, at least seven spoligotypes can be 

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Characterization of clonal complexity in tuberculosis by mycobacterial interspersed repetitive unit-variable-number tandem repeat typing

[HTML] from nih.gov

DG de Viedma, NA Rodriguez, S Andrés… – Journal of clinical …, 2005 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed using primers of a spoligotyping kit (Isogen, The
Netherlands  with the loci showing different alleles within a box) and spoligotypes for the  The two
clones with different MIRU types were indistinguishable by spoligotyping and RFLP (Fig 

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Discrimination of isolates of Mycobacterium bovis in Northern Ireland on the basis of variable numbers of tandem repeats (VNTRs)

RA Skuce, SW McDowell, TR Mallon… – Veterinary …, 2005 – veterinaryrecord.bmj.com

 The isolates were spoligotyped according to the instructions of the manu- facturer (Isogen).  the
inclusion of more than one isolate per herd (VNTR D=0·90, spoligotyping D=0  The difference
between years in the prevalence of VNTR pro- files and spoligotypes was not statistically 

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[HTML] Molecular genotyping of Mycobacterium tuberculosis isolates from Turkey

[HTML] from biomedsearch.com

B Gencer… – Am J Infect Dis, 2005 – biomedsearch.com

 Of the 374 isolates spoligotyped, 87 (23%) displayed unique spoligotypes and 287 (77 
SpoligotypingSpoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen  SpoligotypingSpoligotypes were obtained for 374 isolates (Table 2): 87 isolates (23 

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[CITATION] Epidemiologische Studie bei 109 Tuberkulosefällen im Regierungsbezirk Oberfalz basierend auf Spoligotyping

B Werner, C Ellermeier, L Naumann… – Pneumologie, 2005

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Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues

[PDF] from charite.de

C Schewe, T Goldmann, M Grosser, A Zink… – Virchows Archiv, 2005 – Springer

 All samples with positive amplification of IS6110 were subjected to further analysis by
spoligotyping. The method was performed as described previously [22, 32, 44]. We used a
commercial Spoligotyping Kit (Isogen, Maarsen, The Netherlands, http://www.isogen.nl/). 

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[CITATION] Case-Report: Lungentuberkulose einer MTA–Nachweis von 3 Neuinfektionen durchSpoligotyping

B Werner, C Ellermeier, L Naumann… – Pneumologie, 2005

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Molecular characterization of< i> Mycobacterium tuberculosis</i> isolates in a region of Brazil with a high incidence of tuberculosis

S Borsuk, MM Dellagostin, SG Madeira, C Lima… – Microbes and …, 2005 – Elsevier

 Hybridization of the PCR product to the spoligo-membrane (Isogen Life Sciences) was  The strains
clustered by spoligotyping were analyzed by IS6110-RFLP to confirm any  The spoligotypes were
compared to SpoligoDatabase3 (http://www.pasteur-guadeloupe.fr/tb/spoldb3 

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S< i> 6110</i> DNA fingerprinting analysis of individually separated colonies of< i> Mycobacterium tuberculosis</i>

T Matsumoto, H Ano, T Nagai, K Danno… – Tuberculosis, 2005 – Elsevier

 to the second typing, spoligotypingSpoligotyping membranes were purchased
from Isogen bioscience BV (Bilthoven, The Netherlands). The procedures were
carried out according to the manufacturer’s instructions. Results. 

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Distribution of the Beijing family genotypes of Mycobacterium tuberculosis in Taiwan

[HTML] from nih.gov

R Jou, CY Chiang… – Journal of clinical microbiology, 2005 – Am Soc Microbiol

 Genotyping method.Spoligotyping was used to identify and differentiate the M. tuberculosis
complex isolates in  A commercially available kit (Isogen Bioscience BV, Maarssen, The
Netherlands) was used as  The spoligotypes resolved were M. tuberculosis complex strain specific 

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Characterization of Mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping

[HTML] from nih.gov

WM Prodinger, A Brandstätter… – Journal of clinical …, 2005 – Am Soc Microbiol

 Spoligotyping and IS6110 RFLP analysis.Spoligotyping was carried out as described previously
(23) by using membranes (Isogen Bioscience BV, Maarsen, The Netherlands). The octal codes
for spoligotypes were determined as proposed previously (14). 

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Novos dados sobre os Spoligotypes de estirpes do complexo Mycobacterium tuberculosis isoladas no Hospital Fernando Fonseca (Amadora-Sintra, Portugal)

[TXT] from min-saude.pt

S Davis, V Barros, C Portugal… – Rev Port …, 2005 – repositorio.hff.min-saude.pt

 out in accordance with the recommendations of the mem- brane Kit manufacturer (Isogen
Bioscience BV  contributed the most in this field, seeing that it allows the Spoligotypes to be 
Spoligotyping make it easier to differentiate between the species of the M. tuberculosis complex 

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Efficient discrimination within a Corynebacterium diphtheriae epidemic clonal group by a novel macroarray-based method

[HTML] from nih.gov

I Mokrousov, O Narvskaya… – Journal of clinical …, 2005 – Am Soc Microbiol

 in parallel slots (channels) by using an MN45 miniblotter apparatus (Isogen Biosciences,
Maarssen  both ribotypes of the epidemic clone, while the other 33 spoligotypes included strains 
had unique profiles (Table 2). The discriminatory power of the spoligotyping assessed with 

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[PDF] 结核分枝杆菌散在分布重复单位基因型分型法的应用研究

[PDF] from lung.org.cn

沈国妙, 查佳, 徐琳, 孙斌, 桂晓红, 王奕峰… – 中华结核和呼吸 …, 2005 – lung.org.cn

 3. Spoligotyping 基因分型方法:使用荷兰Isogen Bioscience 公司Spoligotyping 试剂盒并按照厂商提
供的使用手册进行操作。以10 ng 左右的DNA 作 为模板,PCR 法扩增间隔序列。PCR 产物与固定在
膜上的短核苷酸序列杂交,用ECL 系统检测杂交信 号。Spoligotyping 

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Place du spoligotypage lors d’une enquête épidémiologique devant un cas de suspicion de tuberculose pulmonaire nosocomiale

F Jauréguy, MC Gutierez, CJ Marie, C Poirier… – Pathologie Biologie, 2005 – Elsevier

 Keywords: Nosocomial tuberculosis; Epidemiology; Spoligotyping inter-DR ou spacers ont été
synthétisées et immobilisées sur une membrane de nylon commercialisée (Isogen Bioscience
BV, BT  Les résultats des différents spoligotypes obtenus sont présentés dans la Fig. 

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[CITATION] 波兰肺结核患者耐药结核分枝杆菌分子流行病学研究

A Sajduda, A Brzostek, M PopXawska, N Rastogi… – 结核与肺部疾病杂志, 2005

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Recherche de marqueurs génétiques de souches de Mycobactérium turberculosis multirésistantes aux antibiotiques en République Centrafricaine

[PDF] from univ-toulouse.fr

LX Nouvel – 2005 – oatao.univ-toulouse.fr

 61 FIGURE 14 : CARTE DES 41 LOCI MIRU SUR LE CHROMOSOME DE M.
TUBERCULOSIS H37RV ….. 63 FIGURE 15 : DENDROGRAMME DES 79 PROFILS
DE SPOLIGOTYPES DIFFERENTS DES 284 SOUCHES TYPEES. 

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[PDF] Editorial board• EDITORIAL BOARD

[PDF] from pasteur.ac.ir

T Bhatnagar, S Boutaiba… – FEMS …, 2005 – journals2005.pasteur.ac.ir

FEMS MICROBIOLOGY LETTERS Volume 248, Issue 2, Pages 133-274 (15 July 2005) 1. Editorial
board• EDITORIAL BOARD Pages iii-vi Research Letters 2. Lipolytic activity from Halobacteria:
Screening and hydrolase production• SHORT COMMUNICATION Pages 133-140 

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[HTML] SPOLIGOTYPING OF MYCOBACTERIUM BOVIS ISOLATES FOUND IN MANITOBA

[HTML] from nih.gov

C Lutze-Wallace, C Turcotte… – Canadian journal of …, 2005 – ncbi.nlm.nih.gov

 Table I. Table I Summary of spoligotyped isolates by year, species, and location.  types are
656573377603600 (MB-1) and 656473377603600 (MB-2). These spoligotypes have not 
Spoligotyping is particularly useful for differentiating isolates harbouring only a single copy of 

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CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX ISOLATES FROM GREEK PATIENTS WITH SARCOIDOSIS BY SPOLIGOTYPING

[HTML] from nih.gov

M Gazouli, J Ikonomopoulos… – Journal of clinical …, 2005 – Am Soc Microbiol

 hybridization with membrane-bound spacer oligonucleotides and a spoligotyping kit (ISOGEN
Bioscience, Maarsen  The spoligotyping results were entered in a binary format as Excel
spreadsheets  All of the identified spoligotypes have been submitted to the World Spoligotype 

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… MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY ISOLATES FROM HONG KONG AND COMPARISON WITH IS6110 RESTRICTION FRAGMENT LENGTH POLYMORPHISM TYPING AND SPOLIGOTYPING

[HTML] from nih.gov

K Kremer, BKY Au, PCW Yip, R Skuce… – Journal of clinical …, 2005 – Am Soc Microbiol

 In addition to spoligotyping with a commercial membrane containing the standard set of 43 spacer
oligonucleotides (Isogen Bioscience BV, Maarssen, The  (35), who also subjected Beijing strains
to spoligotyping with novel spacers, at least seven spoligotypes can be 

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RECHERCHE DE MARQUEURS GÉNÉTIQUES DE SOUCHES DE MYCOBACTÉRIUM TURBERCULOSIS MULTIRÉSISTANTES AUX ANTIBIOTIQUES EN RÉPUBLIQUE CENTRAFRICAINE

[PDF] from univ-toulouse.fr

LX Nouvel – 2005 – oatao.univ-toulouse.fr

 61 FIGURE 14 : CARTE DES 41 LOCI MIRU SUR LE CHROMOSOME DE M.
TUBERCULOSIS H37RV ….. 63 FIGURE 15 : DENDROGRAMME DES 79 PROFILS
DE SPOLIGOTYPES DIFFERENTS DES 284 SOUCHES TYPEES. 

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[CITATION] CASE-REPORT: LUNGENTUBERKULOSE EINER MTA–NACHWEIS VON 3 NEUINFEKTIONEN DURCHSPOLIGOTYPING

B Werner, C Ellermeier, L Naumann… – Pneumologie, 2005

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[PDF] EDITORIAL BOARD• EDITORIAL BOARD

[PDF] from pasteur.ac.ir

T Bhatnagar, S Boutaiba… – FEMS …, 2005 – journals2005.pasteur.ac.ir

FEMS MICROBIOLOGY LETTERS Volume 248, Issue 2, Pages 133-274 (15 July 2005) 1. Editorial
board• EDITORIAL BOARD Pages iii-vi Research Letters 2. Lipolytic activity from Halobacteria:
Screening and hydrolase production• SHORT COMMUNICATION Pages 133-140 

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*************************************************************

2004

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PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers

[HTML] from nih.gov

R Kotłowski, IC Shamputa, NA El Aila… – Journal of clinical …, 2004 – Am Soc Microbiol

 In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). 

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[HTML] PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers

[HTML] from ukpmc.ac.uk

R Kotlowski, IC Shamputa, NA El Aila… – JOURNAL OF …, 2004 – ukpmc.ac.uk

 In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). 

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Molecular fingerprinting of Mycobacterium bovis subsp. caprae isolates from Central Europe

[HTML] from nih.gov

W Erler, G Martin, K Sachse… – Journal of clinical …, 2004 – Am Soc Microbiol

 method (12) and subjected to direct-repeat-specific PCR and hybridization with membrane-bound
spacer oligonucleotides and a spoligotyping kit (ISOGEN Bioscience, Maarsen, The  Spoligotyping
of the 79 isolates revealed 11 different spoligotypes (designated C1 to 

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Analysis of clonal composition of Mycobacterium tuberculosis isolates in primary infections in children

[HTML] from nih.gov

DG de Viedma, M Marín, MJ Ruiz… – Journal of clinical …, 2004 – Am Soc Microbiol

 The PCR for amplification of the DR region was performed with the primers in the
spoligotyping kit (Isogen, Maarsen, The Netherlands) and according to the instructions of
the manufacturer. All spoligotypes were confirmed by repeating the assay. 

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Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain

[HTML] from nih.gov

A Aranaz, B Romero, N Montero… – Journal of clinical …, 2004 – Am Soc Microbiol

 The test was carried out with membranes obtained from Isogen Bioscience BV, Maarssen, The 
associated with the DR locus and their influence on the interpretation of spoligotyping
corresponding to relapses or infections at different sites showed identical spoligotypes (6, 10, 

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Bovine tuberculosis (Mycobacterium bovis) in wildlife in Spain

[HTML] from nih.gov

A Aranaz, L De Juan, N Montero… – Journal of Clinical …, 2004 – Am Soc Microbiol

 of the biotin-labeled PCR product onto a spoligotyping membrane (Isogen Bioscience BV 
Schematic representation of spoligotyping patterns identified among wildlife and areas where
the patterns  Six spoligotypes were identified among the isolates from Doñana National Park. 

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[PDF] Simultaneous detection and typing of Mycobacterium tuberculosis complex bacteria

[PDF] from cli-online.com

M de Boer, A van der Zanden… – cli-online.com

 of the mem- brane or by specific imaging systems developed by Isogen Life Science  in the
laboratory, because different M. tuberculosis strains or DNA can yield different spoligotypes. Thus
spoligotyping can be used for quality control of microscopy of AFB, cultures or nucleic 

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Transmission of tuberculosis from smear negative patients: a molecular epidemiology study

[HTML] from highwire.org

E Hernandez-Garduno, V Cook, D Kunimoto… – Thorax, 2004 – thorax.highwire.org

 A spoligotyping kit (Isogen Bioscience, Maarssen) was used as a secondary typing
technique for strains with five copies or less of IS6110. 13 Smear specimens were
stained with Auramine O and confirmed by Ziehl Neelsen staining. 

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Characterization of ancestral Mycobacterium tuberculosis by multiple genetic markers and proposal of genotyping strategy

[HTML] from nih.gov

YJ Sun, ASG Lee, ST Ng, S Ravindran… – Journal of clinical …, 2004 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen, The Netherlands) according to the instructions of the manufacturer. The spoligotypes
were presented by using pseudo-original patterns or 15 octal codes, as described by 

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Genotypic and phenotypic heterogeneity among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients

[HTML] from nih.gov

IC Shamputa, L Rigouts, LA Eyongeta… – Journal of clinical …, 2004 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed with a commercial kit (Isogen Bioscience BV,
Maarssen, The  All 10 clones from each of the 97 patients were tested by spoligotyping of the 97
patients tested, including the 7 Beijing genotype strains, showed identical spoligotypes

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Relating the size of molecularly defined clusters of tuberculosis to the duration of symptoms

[HTML] from oxfordjournals.org

TP Giordano, H Soini, LD Teeter… – Clinical infectious …, 2004 – cid.oxfordjournals.org

 BioImage). Isolates containing <5 IS6110 elements were spoligotyped [27] with a
commercially available kit (Isogen Bioscience BV). Spoligotyping is an alternative
method for molecular characterization of M. tuberculosis isolates. 

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Molecular epidemiology of drug-resistant Mycobacterium tuberculosis strains isolated from patients with pulmonary tuberculosis in Poland: a 1-year study

A Sajduda, A Brzostek, M Poplawska… – … of Tuberculosis and …, 2004 – ingentaconnect.com

 Spoligotyping was per- formed using a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) accord- ing to the  In accordance with previous findings, spoligotyping was less
discrimina- tory than IS6110 RFLP.8,9 A total of 91 spoligotypes 

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[CITATION] КРАТКИЕ СООБЩЕНИЯ. СПОЛИГОТИПИРОВАНИЕ КУЛЬТУР МИКОБАКТЕРИЙ ТУБЕРКУЛЕЗА, ВЫДЕЛЕННЫХ ОТ БОЛЬНЫХ ИЗ САМАРСКОЙ …

Е Желткова, Я Балабанова… – Zhurnal …, 2004 – Redaktsiia zhurnala” Zhurnal …

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Transmission characteristics of tuberculosis in the foreign-born and the Canadian-born populations of Alberta, Canada

D Kunimoto, K Sutherland… – … of Tuberculosis and …, 2004 – ingentaconnect.com

 Two or more isolates with the same DNA fingerprint were called a cluster. A
spoligotyping kit (Isogen Bioscience, Maarssen, The Netherlands) was used for
secondary analysis of all strains with five copies or less of IS6110.6 

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Species differentiation and antibiotic susceptibility testing with DNA microarrays

[PDF] from aseanbiotechnology.info

G Vernet, C Jay, M Rodrigue… – Journal of applied …, 2004 – Wiley Online Library

 (Please refer to Fig. 3a.). 4.2.1 Reference spoligotyping. (Please refer to Fig.  (1997). Reagents
were provided by Isogen Life Science (The Netherlands).  4.2.2 Comparison of spoligotypes
obtained by dot-blot and by the microarray with a strain of M. bovis. (Please refer to Fig. 

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High rates of clustering of strains causing tuberculosis in Harare, Zimbabwe: a molecular epidemiological study

[HTML] from nih.gov

PJ Easterbrook, A Gibson, S Murad… – Journal of clinical …, 2004 – Am Soc Microbiol

 primer 5′-CCAAGAGGGGACGGAAAC-3′ and biotinylated primer 5′-GGTTTTGGGTCTG
ACGAC-3′). The PCR products were then hybridized to a Biodyne C membrane (Isogen
Bioscience, Maarsen  By using spoligotyping alone, 54 distinct spoligotypes were identified 

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Identification of MDR-TB Beijing/W and other Mycobacterium tuberculosis genotypes in Nairobi, Kenya

WA Githui, AM Jordaan, ES Juma… – … of Tuberculosis and …, 2004 – ingentaconnect.com

 Spoligotyping and analysis The method is based on in vitro PCR amplification of the  Membranes
and primers were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands.  were scanned
and added to an existing spoligotype data- base containing spoligotypes from all 

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First isolation of< i> Mycobacterium microti</i>(Llama-type) from a dog

L Deforges, HJ Boulouis, JL Thibaud… – Veterinary …, 2004 – Elsevier

 Definitive subspecies identification was established by spoligotyping (for “Spacer Oligotyping 
Easthill, NY, USA) sensitised with 43 aminolink–oligonucleotides, according to the procedures
recommended by the manufacturer of the oligonucleotides (Isogen Bioscience, Maarssen 

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Impact of drug resistance on fitness of Mycobacterium tuberculosis strains of the W‐Beijing genotype

OS Toungoussova, DA Caugant… – FEMS Immunology …, 2004 – Wiley Online Library

 20,21]. Spoligotyping was performed by using a commercially available kit (Isogen
Bioscience BV, Maarssen, The Netherlands) according to the instructions supplied
by the manufacturer, as previously described [22]. M. tuberculosis 

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6 Molecular Epidemiology of Mycobacterium bow’s

RA SKUCE… – Tuberculosis, 2004 – books.google.com

 e Spoligotyping result of M. tuberculosis H37Rv and M. bovis field isolates (rows), A membrane 
Courtesy of Isogen Bioscience BV representative panels of M. tuberculosis complex bac- teria
(van  2000) and relatively large databases of spoligotypes are being curated (Sola et al. 

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Genomic characterization of an endemic Mycobacterium tuberculosis strain: evolutionary and epidemiologic implications

[HTML] from nih.gov

D Nguyen, P Brassard, D Menzies… – Journal of clinical …, 2004 – Am Soc Microbiol

 Spoligotyping (or spacer oligonucleotide typing) was done by using a PCR-based technique
which detects the presence or absence of 43 unique sequence spacers in the direct repeat
(DR) locus (Isogen Bioscience BV, Maarssen, The Netherlands) (20). 

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[HTML] Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques

[HTML] from scielo.br

PM Parreiras, FCF Lobato, AP Alencar… – Memórias do Instituto …, 2004 – SciELO Brasil

 2002), applied on the membrane using a Miniblotter MN45 (Isogen, the Netherlands) and after
1 min incubation at RT, the membrane was  This observation was confirmed by the generation
of M. bovis genotypes after submitting the isolates to spoligotyping (data not shown). 

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[PDF] Antimicrobial resistance among Campylobacter strains, United States, 1997–2001

[PDF] from cdc.gov

A Gupta, JM Nelson, TJ Barrett, RV Tauxe… – Emerg Infect …, 2004 – wwwnc.cdc.gov

Page 130. We summarize antimicrobial resistance surveillance data in human and
chicken isolates of Campylobacter. Isolates were from a sentinel county study from
1989 through 1990 and from nine state health departments 

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Multicenter evaluation of reverse line blot assay for detection of drug resistance in Mycobacterium tuberculosis clinical isolates

[PDF] from rivm.nl

I Mokrousov, NV Bhanu, PN Suffys… – Journal of …, 2004 – Elsevier

 Furthermore, the proposed method requires the same simple equipment as that used for
spoligotyping and permits simultaneous analysis of  Biodyne ®C membrane (Pall Gelman
Laboratory, Ann Arbor, MI, USA) using the MN45 miniblotter apparatus (Isogen Biosci., Maarssen 

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PCR-BASED GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS WITH NEW GC-RICH REPEATED SEQUENCES AND IS6110 INVERTED REPEATS USED AS PRIMERS

[HTML] from nih.gov

R Kotłowski, IC Shamputa, NA El Aila… – Journal of clinical …, 2004 – Am Soc Microbiol

 In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). 

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ANALYSIS OF CLONAL COMPOSITION OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN PRIMARY INFECTIONS IN CHILDREN

[HTML] from nih.gov

DG de Viedma, M Marín, MJ Ruiz… – Journal of clinical …, 2004 – Am Soc Microbiol

 The PCR for amplification of the DR region was performed with the primers in the
spoligotyping kit (Isogen, Maarsen, The Netherlands) and according to the instructions of
the manufacturer. All spoligotypes were confirmed by repeating the assay. 

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[HTML] PCR-BASED GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS WITH NEW GC-RICH REPEATED SEQUENCES AND IS6110 INVERTED REPEATS USED AS PRIMERS

[HTML] from ukpmc.ac.uk

R Kotlowski, IC Shamputa, NA El Aila… – JOURNAL OF …, 2004 – ukpmc.ac.uk

 In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). 

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SPOLIGOTYPING PROFILE CHANGE CAUSED BY DELETION OF A DIRECT VARIABLE REPEAT IN A MYCOBACTERIUM TUBERCULOSIS ISOGENIC LABORATORY STRAIN

[HTML] from nih.gov

A Aranaz, B Romero, N Montero… – Journal of clinical …, 2004 – Am Soc Microbiol

 The test was carried out with membranes obtained from Isogen Bioscience BV, Maarssen, The 
associated with the DR locus and their influence on the interpretation of spoligotyping
corresponding to relapses or infections at different sites showed identical spoligotypes (6, 10, 

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BOVINE TUBERCULOSIS (MYCOBACTERIUM BOVIS) IN WILDLIFE IN SPAIN

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A Aranaz, L De Juan, N Montero… – Journal of Clinical …, 2004 – Am Soc Microbiol

 of the biotin-labeled PCR product onto a spoligotyping membrane (Isogen Bioscience BV 
Schematic representation of spoligotyping patterns identified among wildlife and areas where
the patterns  Six spoligotypes were identified among the isolates from Doñana National Park. 

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TRANSMISSION OF TUBERCULOSIS FROM SMEAR NEGATIVE PATIENTS: A MOLECULAR EPIDEMIOLOGY STUDY

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E Hernandez-Garduno, V Cook, D Kunimoto… – Thorax, 2004 – thorax.highwire.org

 A spoligotyping kit (Isogen Bioscience, Maarssen) was used as a secondary typing
technique for strains with five copies or less of IS6110. 13 Smear specimens were
stained with Auramine O and confirmed by Ziehl Neelsen staining. 

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CHARACTERIZATION OF ANCESTRAL MYCOBACTERIUM TUBERCULOSIS BY MULTIPLE GENETIC MARKERS AND PROPOSAL OF GENOTYPING STRATEGY

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YJ Sun, ASG Lee, ST Ng, S Ravindran… – Journal of clinical …, 2004 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen, The Netherlands) according to the instructions of the manufacturer. The spoligotypes
were presented by using pseudo-original patterns or 15 octal codes, as described by 

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GENOTYPIC AND PHENOTYPIC HETEROGENEITY AMONG MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PULMONARY TUBERCULOSIS PATIENTS

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IC Shamputa, L Rigouts, LA Eyongeta… – Journal of clinical …, 2004 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed with a commercial kit (Isogen Bioscience BV,
Maarssen, The  All 10 clones from each of the 97 patients were tested by spoligotyping of the 97
patients tested, including the 7 Beijing genotype strains, showed identical spoligotypes

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HIGH RATES OF CLUSTERING OF STRAINS CAUSING TUBERCULOSIS IN HARARE, ZIMBABWE: A MOLECULAR EPIDEMIOLOGICAL STUDY

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PJ Easterbrook, A Gibson, S Murad… – Journal of clinical …, 2004 – Am Soc Microbiol

 primer 5′-CCAAGAGGGGACGGAAAC-3′ and biotinylated primer 5′-GGTTTTGGGTCTG
ACGAC-3′). The PCR products were then hybridized to a Biodyne C membrane (Isogen
Bioscience, Maarsen  By using spoligotyping alone, 54 distinct spoligotypes were identified 

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2003

Evaluation of the epidemiologic utility of secondary typing methods for differentiation of Mycobacterium tuberculosis isolates

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A Kwara, R Schiro, LS Cowan… – Journal of clinical …, 2003 – Am Soc Microbiol

 Spoligotyping (Isogen Bioscience BV, Maarssen, The Netherlands) was performed in accordance
with previously published methods (8). Results were recorded as an octal code (4). MIRU loci
(10) were amplified with primers specific for sequences flanking each locus (Table 1 

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Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies byspoligotyping

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AR Zink, C Sola, U Reischl, W Grabner… – Journal of clinical …, 2003 – Am Soc Microbiol

 For this analysis, we used a commercial spoligotyping kit (Isogen, Maarsen, The Netherlands 
it has been shown that the absence of spacers 33 to 36 in the spoligotyping signatures of  absence
of spacers 33 to 36 (Table 4), most of the M. tuberculosis spoligotypes obtained from 

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Characterization of Finnish Mycobacterium tuberculosis isolates by spoligotyping

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K Puustinen, M Marjamäki, N Rastogi… – Journal of clinical …, 2003 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) according to the instructions of the manufacturer.  We analyzed 380 M.
tuberculosis isolates by spoligotyping and obtained a total of 138 spoligotypes

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Widespread pyrazinamide-resistant Mycobacterium tuberculosis family in a low-incidence setting

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D Nguyen, P Brassard, J Westley… – Journal of clinical …, 2003 – Am Soc Microbiol

 IS6110 RFLP by Southern blotting (38) and spoligotyping (Isogen Bioscience BV) (26) were
performed using  the observation that the DNA fingerprints by both IS6110 RFLP and spoligotyping
are similar  approximately 2 to 3.2 years (13, 42) and that of spoligotypes is believed to 

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Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates in the Archangel prison in Russia: predominance of the W-Beijing clone family

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OS Toungoussova, A Mariandyshev… – Clinical infectious …, 2003 – cid.oxfordjournals.org

 Spoligotyping was performed using a commercially available spoligotyping kit (Isogen Bioscience
BV) in  were represented by 6 and 3 isolates each, and 4 spoligotypes comprised 2  All isolates
with identical IS6110 RFLP patterns were found to be identical by spoligotyping

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Molecular typing of Mycobacterium tuberculosis by using nine novel variable-number tandem repeats across the Beijing family and low-copy-number IS6110 isolates

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RS Spurgiesz, TN Quitugua, KL Smith… – Journal of clinical …, 2003 – Am Soc Microbiol

 In a database of 3,319 spoligotypes from isolates collected in geographically distinct areas, seven
spoligotype  Advanced Nucleic Acids Technology Core Facility, San Antonio, Tex.) and
commercially available spoligotyping membranes (Isogen, Maarssen, The 

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The molecular epidemiology of tuberculosis in western Canada

JM FitzGerald, A Fanning… – … of Tuberculosis and …, 2003 – ingentaconnect.com

 All isolates matched as identical by computer were manually confirmed by visually comparing
the original autoradiographs. A spoligotyping kit (Isogen Bioscience, Maarssen, The Netherlands)
was used for secondary analysis of 90 strains with five copies or less of IS6110. 

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Genetic biodiversity of Mycobacterium tuberculosis complex strains from patients with pulmonary tuberculosis in Cameroon

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SN Niobe-Eyangoh, C Kuaban, P Sorlin… – Journal of clinical …, 2003 – Am Soc Microbiol

 (i) Spoligotyping.Spoligotyping was performed  as a probe to hybridize with 43 synthetic spacer
oligonucleotides covalently bound to a membrane (Isogen Biosciences BV  Spoligotypes were
analyzed with the Bionumerics program version 2.5 (Applied Maths, Kortrijk, Belgium). 

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Detection of pathogenic mycobacteria of veterinary importance

RA Skuce, MS Hughes, MJ Taylor… – METHODS IN MOLECULAR …, 2003 – Springer

 2.5.3. Membrane-Based Analysis 1. Spoligotyping Kit (Isogen Bioscience BV, Maarssen,
Netherlands). 2. SSPE buffer (2X): 1X SSPE is 0.18 M HCl, 10 mM NaH2PO4, 1 mM EDTA.
To be supplemented with 0.1 % sodium dodecyl sulfate (SDS). 

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Analysis of multidrug-resistant Mycobacterium bovis from three clinical samples from Scotland

VM Hughes, R Skuce, C Doig… – … of Tuberculosis and …, 2003 – ingentaconnect.com

 from each isolate was subjected to spoligotyping,11 exactly as specified by the kit manufacturers
(Isogen Bioscience BV  linked as they shared spoligotyping and DRr-RFLP profiles. The DRr-RFLP
profiles and spoligotypes shared by Cases I/94 and II/98 have never previously 

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[PDF] ÇOĞUL DİRENÇLİ MYCOBACTERİUM TUBERCULOSİS İZOLATLARINDA SEKONDER İLAÇLARA DİRENÇ VE

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E TAKİP – 2003 – uvt.ulakbim.gov.tr

 Spoligotyping için ticari kit kullanılmıştır (Isogen Bioscience BV, Maarssen, The Netherlands  in
Cuba outside of Havana, July 1994- June 1995: utility of spoligotyping versus IS6110  BN, Kremer
K., Valetudie G. et al, Global distribution of Mycobacterium tuberculosis spoligotypes

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Investigations into an outbreak of tuberculosis in a flock of sheep in contact with tuberculous cattle

FE Malone, EC Wilson, JM Pollock… – Journal of Veterinary …, 2003 – Wiley Online Library

 Molecular strain typing was undertaken on 39 cattle and three sheep M. bovis isolates using
spoligotyping (Kamerbeek et al., 1997) exactly as directed by the manufacturer (Isogen Bioscience,
Netherlands) and a selection of six VNTR markers (Skuce and Neill, 2001). 

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IS6110-mediated deletion polymorphism in the direct repeat region of clinical isolates of Mycobacterium tuberculosis

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SL Sampson, RM Warren, M Richardson… – Journal of …, 2003 – Am Soc Microbiol

 These isolates were chosen on the basis of spoligotypes, which indicated a deletion of 
Spoligotyping.DNA polymorphism in the DR locus was detected by spoligotyping according to
 products were then hybridized to a commercially available membrane (Isogen Biosciences 

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Heterogeneity of Mycobacterium tuberculosis isolates in Yangon, Myanmar

S Phyu, R Jureen, T Ti, UR Dahle… – Journal of clinical …, 2003 – Am Soc Microbiol

 susceptibility test, niacin accumulation test, nitrate reduction test, and pyrazinamidase activity)
were performed for phenotyping (4). Spoligotyping was performed with a commercial kit as
recommended by the manufacturer (Isogen Bioscience BV, Maarssen, The Netherlands). 

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Single nucleotide polymorphisms in genes associated with isoniazid resistance in Mycobacterium tuberculosis

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SV Ramaswamy, R Reich, SJ Dou… – Antimicrobial agents …, 2003 – Am Soc Microbiol

 Spoligotyping and major genetic group assignment.Low-copy-number isolates were subjected
to a secondary typing method by use of spoligotyping. This method was performed with a
commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to 

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Apport épidémiologique du typage moléculaire des bacilles de la tuberculose

V Vincent… – Médecine et Maladies Infectieuses, 2003 – Elsevier

 à partir d’un lysat cellulaire utilisé comme source d’ADN, puis hybridée à une membrane sur
laquelle sont fixés les 43 séquences inter DR les plus fréquentes (la membrane est
commercialisée par Isogen Bioscience BV, Maarsen, Hollande) (Fig. 2). Le spoligotyping est 

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[CITATION] Multicenter Evaluation of Reverse Line Blot Assay to Predict Drug Resistance in Mycobacterium tuberculosis Clinical Isolates

I Mokrousov, NV Bhanu, PN Suffys, GV Kadival…

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EVALUATION OF THE EPIDEMIOLOGIC UTILITY OF SECONDARY TYPING METHODS FOR DIFFERENTIATION OF MYCOBACTERIUM TUBERCULOSIS ISOLATES

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A Kwara, R Schiro, LS Cowan… – Journal of clinical …, 2003 – Am Soc Microbiol

 Spoligotyping (Isogen Bioscience BV, Maarssen, The Netherlands) was performed in accordance
with previously published methods (8). Results were recorded as an octal code (4). MIRU loci
(10) were amplified with primers specific for sequences flanking each locus (Table 1 

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CHARACTERIZATION OF FINNISH MYCOBACTERIUM TUBERCULOSIS ISOLATES BY SPOLIGOTYPING

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K Puustinen, M Marjamäki, N Rastogi… – Journal of clinical …, 2003 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) according to the instructions of the manufacturer.  We analyzed 380 M.
tuberculosis isolates by spoligotyping and obtained a total of 138 spoligotypes

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WIDESPREAD PYRAZINAMIDE-RESISTANT MYCOBACTERIUM TUBERCULOSIS FAMILY IN A LOW-INCIDENCE SETTING

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D Nguyen, P Brassard, J Westley… – Journal of clinical …, 2003 – Am Soc Microbiol

 IS6110 RFLP by Southern blotting (38) and spoligotyping (Isogen Bioscience BV) (26) were
performed using  the observation that the DNA fingerprints by both IS6110 RFLP and spoligotyping
are similar  approximately 2 to 3.2 years (13, 42) and that of spoligotypes is believed to 

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CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX DNAS FROM EGYPTIAN MUMMIES BYSPOLIGOTYPING

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AR Zink, C Sola, U Reischl, W Grabner… – Journal of clinical …, 2003 – Am Soc Microbiol

 For this analysis, we used a commercial spoligotyping kit (Isogen, Maarsen, The Netherlands 
it has been shown that the absence of spacers 33 to 36 in the spoligotyping signatures of  absence
of spacers 33 to 36 (Table 4), most of the M. tuberculosis spoligotypes obtained from 

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2002

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Tuberculosis recurrences: reinfection plays a role in a population whose clinical/epidemiological characteristics do not favor reinfection

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DG de Viedma, M Marin, S Hernangomez… – Archives of internal …, 2002 – Am Med Assoc

 The polymerase chain reaction (PCR) for amplifying the directed repeats region was performed
using primers of the spoligotyping kit (Isogen Bioscience, Maarssen, the Netherlands), following
the manufacturer’s instructions. All spoligotypes showing differences for the strains 

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[HTML] Molecular differentiation of Mycobacterium tuberculosis strains without IS6110 insertions

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KH Lok, WH Benjamin Jr, ME Kimerling… – Emerging infectious …, 2002 – ncbi.nlm.nih.gov

 Spoligotyping membranes were purchased from Isogen Bioscience BV (Isogen, Bilthoven, the
Netherlands).  repeats results of three zero-copy IS6110 isolates with identical spoligotyping and
polymorphic  Fourteen of 32 spoligotyped strains are similar and have the same CDC 

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Variable-number tandem repeat typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110 by using mycobacterial interspersed repetitive units

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LS Cowan, L Mosher, L Diem… – Journal of clinical …, 2002 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen, The  Q could also be divided by IS6110 RFLP and spoligotyping into subclusters
characterized by different insertion sites and divergent spoligotypes, as described 

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Infection of red deer, cattle, and humans with Mycobacterium bovis subsp. caprae in western Austria

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WM Prodinger, A Eigentler… – Journal of clinical …, 2002 – Am Soc Microbiol

 on membranes with covalently bound, spacer sequence-specific oligonucleotides purchased
from Isogen Bioscience BV  region I isolates (3 cattle, 1 deer); lanes 5 to 9, spoligotypes from four 
wax-embedded tissue from that animal yielded the region II spoligotyping pattern (Fig. 

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Transmission of drug-resistant tuberculosis in Texas and Mexico

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TN Quitugua, BJ Seaworth, SE Weis… – Journal of clinical …, 2002 – Am Soc Microbiol

 Locally synthesized and biotinylated PCR primers (Advanced Nucleic Acids Technology
Core Facility, UTHSCSA) were used with commercially available spoligotyping
membranes (Isogen, Maarssen, The Netherlands). Spoligotyping 

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Spread of drug-resistant Mycobacterium tuberculosis strains of the Beijing genotype in the Archangel Oblast, Russia

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OS Toungoussova, P Sandven… – Journal of clinical …, 2002 – Am Soc Microbiol

 Spoligotyping was performed by using a commercially available kit (Isogen Bioscience BV 
Spoligotyping.The spoligotyping method was used as an additional tool to further  to the Beijing
genotype by their IS6110 RFLP patterns showed identical spoligotypes, lacking spacers 1 

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[HTML] Molecular typing of Mycobacterium tuberculosis strains with a common two-band IS6110 pattern

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KH Lok, WH Benjamin Jr, ME Kimerling… – Emerging infectious …, 2002 – ncbi.nlm.nih.gov

 Spoligotyping membranes were used according to manufacturer’s recommendations (Isogen
Bioscience BV, Bilthoven  well between these sizes, which could result in different spoligotypes
of a  Using VNTR and spoligotyping techniques to reexamine some of these clusters, we 

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Sputum induction problems identified through genetic fingerprinting

B Nivin, T O’Flaherty, E Leibert, BY Zhao… – Infection control and …, 2002 – JSTOR

 Because spoligotyping is less discriminatory than IS6110-based analysis, identical spoligotypes
may indi- cate the need for further confirmation using the latter method.  The spoligotyping analysis
was performed using a commercially available kit (Isogen Bioscience BV 

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Isoniazid resistance, mycobacterial genotype and outcome in Vietnamese adults with tuberculous meningitis

GE Thwaites, TTH Chau, M Caws… – … of Tuberculosis and …, 2002 – ingentaconnect.com

 was car- ried out in accordance with a standardised protocol for spoligotyping.16 Briefly  were
heat-denatured and hybridised to the spoligotype membrane (Isogen Biosciences, Asmaars 
Patterns were compared against the Beijing and ‘Vietnam’ spoligotypes described by Anh et 

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Biochemical and genetic evidence for the transfer of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to the species Mycobacterium bovis Karlson and …

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S Niemann, E Richter… – International journal of …, 2002 – Soc General Microbiol

 Spoligotyping, which is based on the detection of various non- repetitive spacer sequences located
between small repetitive units (direct  blotting technique, was performed as described previously
(Kamerbeek et al., 1997) by using a commercially available membrane (Isogen). 

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Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates from Ethiopian pulmonary tuberculosis patients with and without human …

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J Bruchfeld, G Aderaye, IB Palme… – Journal of clinical …, 2002 – Am Soc Microbiol

 synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience
BV  24, 41); and they all lacked the typical M. canetti spoligotyping patterns (presence of  3.
Schematic representation of the spoligotypes of M. tuberculosis isolates belonging to four 

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[HTML] Genotyping analyses of tuberculosis cases in US-and foreign-born Massachusetts residents

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S Sharnprapai, AC Miller, R Suruki… – Emerging infectious …, 2002 – ncbi.nlm.nih.gov

 with the molecular weight standards provided by CDC. Spoligotyping was performed
with a commercially available kit, in accordance with the manufacturer’s instructions
(Isogen Bioscience BV, Maarseen, the Netherlands). 

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[HTML] Statewide Molecular Epidemiology of Mycobacterium tuberculosis Transmission in a Moderate-to Low-Incidence State: Are Contact Investigations Enough?

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WA Cronin, JE Golub, MJ Lathan… – Emerging Infectious …, 2002 – ncbi.nlm.nih.gov

 Spoligotyping was performed for all matching strains that had six or more IS6110
copies by using a commercially available kit, according to the manufacturer’s
instructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13). 

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Tuberculosis

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Q Eichbaum… – American Journal of Clinical …, 2002 – ajcp.ascpjournals.org

 Quentin Eichbaum, MD, PhD, MPH,1 and Eric J. Rubin, MD, PhD2 Key Words: Susceptibility;
Rapid culture; Molecular beacons; Nested PCR; Ligation; Spoligotyping; Strains DOI:
10.1309/XX1NBANX8JKMTUQN Page 2. Eichbaum and Rubin / TUBERCULOSIS 

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[HTML] STATEWIDE MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS TRANSMISSION IN A MODERATE-TO LOW-INCIDENCE STATE: ARE CONTACT INVESTIGATIONS ENOUGH?

[HTML] from nih.gov

WA Cronin, JE Golub, MJ Lathan… – Emerging Infectious …, 2002 – ncbi.nlm.nih.gov

 Spoligotyping was performed for all matching strains that had six or more IS6110
copies by using a commercially available kit, according to the manufacturer’s
instructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13). 

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BIOCHEMICAL AND GENETIC EVIDENCE FOR THE TRANSFER OF MYCOBACTERIUM TUBERCULOSIS SUBSP. CAPRAE ARANAZ ET AL. 1999 TO THE SPECIES MYCOBACTERIUM BOVIS KARLSON AND …

[PDF] from sgmjournals.org

S Niemann, E Richter… – International journal of …, 2002 – Soc General Microbiol

 Spoligotyping, which is based on the detection of various non- repetitive spacer sequences located
between small repetitive units (direct  blotting technique, was performed as described previously
(Kamerbeek et al., 1997) by using a commercially available membrane (Isogen). 

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2001

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Transmission of Mycobacterium tuberculosis to a Funeral Director During Routine Embalming*

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M Lauzardo, P Lee, H Duncan… – Chest, 2001 – chestjournal.chestpubs.org

 Secondary strain typing of M tuberculosis was performed by spoligotyping (Isogen
Bioscience, BV; Maarssen, The Netherlands) as described by Kamerbeek et al. 10
Spacer regions within the direct repeat locus of the chromosome 

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Epidemiologic Usefulness of Spoligotyping for Secondary Typing ofMycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110

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WA Cronin, JE Golub, LS Magder… – Journal of clinical …, 2001 – Am Soc Microbiol

 Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered
strains  investigation of low-copy clusters to those with rare spoligotypes may also  In conclusion,
spoligotyping did not appreciably improve strain differentiation among isolates with 

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Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present

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BM Rothschild, LD Martin, G Lev… – Clinical Infectious …, 2001 – cid.oxfordjournals.org

 The sequence was analyzed in an ABI PRISM 310 automated sequencer (PE Applied Biosystems).
Spoligotyping. To determine the species of Mycobacterium within the M. tuberculosis complex,
the spoligotyping system (Isogen) was applied in the Jerusalem laboratory. 

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Spread of drug-resistant pulmonary tuberculosis in Estonia

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A Krüüner, SE Hoffner, H Sillastu… – Journal of clinical …, 2001 – Am Soc Microbiol

 Membranes for spoligotyping were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. 
Genetic homogenity of family A confirmed by spoligotyping.The 12 strains from family A showed
an  The spoligotypes of the non-Beijing family strains were highly diverse (Table 2 

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Transmission dynamics and molecular characterization of Mycobacterium tuberculosis isolates with low copy numbers of IS6110

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H Soini, X Pan, L Teeter, JM Musser… – Journal of clinical …, 2001 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV,
Maarssen, The Netherlands) according to the instructions of the manufacturer. 
Spoligotyping.A total of 72 spoligotypes was identified among the 377 isolates. 

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Identification of a contaminating Mycobacterium tuberculosis strain with a transposition of an IS6110insertion element resulting in an altered spoligotype

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WH Benjamin Jr, KH Lok, R Harris… – Journal of clinical …, 2001 – Am Soc Microbiol

 36-bp direct repeats of M. tuberculosis, was done using membranes from Isogen Biosciences
BV  of direct repeat regions and thus loss of spacers on spoligotyping have been  The second report
explains differences between two common spoligotypes found in the IS6110 Harlem 

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Spoligotype diversity of Mycobacterium bovis strains isolated in France from 1979 to 2000

[HTML] from nih.gov

N Haddad, A Ostyn, C Karoui… – Journal of clinical …, 2001 – Am Soc Microbiol

 “None” corresponds to departments where two or more spoligotypes were codominant  (6), was
used for the spoligotyping of M  of 43 aminolink oligonucleotides by following the procedures
recommended by the manufacturer of the oligonucleotides (Isogen Bioscience, Maarssen 

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Risk of Mycobacterium tuberculosis transmission in a low-incidence country due to immigration from high-incidence areas

[HTML] from nih.gov

T Lillebaek, ÅB Andersen, J Bauer… – Journal of clinical …, 2001 – Am Soc Microbiol

 For spoligotyping, DNA extraction was performed by mechanical disruption of the cells with glass
beads by vortexing either a few colonies from solid media or 100 μl of the frozen culture stock
(27). The membranes used (Isogen; Bioscience BV, Utrecht, The Netherlands 

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TRANSMISSION OF MYCOBACTERIUM TUBERCULOSIS TO A FUNERAL DIRECTOR DURING ROUTINE EMBALMING*

[HTML] from chestpubs.org

M Lauzardo, P Lee, H Duncan… – Chest, 2001 – chestjournal.chestpubs.org

 Secondary strain typing of M tuberculosis was performed by spoligotyping (Isogen
Bioscience, BV; Maarssen, The Netherlands) as described by Kamerbeek et al. 10
Spacer regions within the direct repeat locus of the chromosome 

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EPIDEMIOLOGIC USEFULNESS OF SPOLIGOTYPING FOR SECONDARY TYPING OFMYCOBACTERIUM TUBERCULOSIS ISOLATES WITH LOW COPY NUMBERS OF IS6110

[HTML] from nih.gov

WA Cronin, JE Golub, LS Magder… – Journal of clinical …, 2001 – Am Soc Microbiol

 Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered
strains  investigation of low-copy clusters to those with rare spoligotypes may also  In conclusion,
spoligotyping did not appreciably improve strain differentiation among isolates with 

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MYCOBACTERIUM TUBERCULOSIS COMPLEX DNA FROM AN EXTINCT BISON DATED 17,000 YEARS BEFORE THE PRESENT

[HTML] from oxfordjournals.org

BM Rothschild, LD Martin, G Lev… – Clinical Infectious …, 2001 – cid.oxfordjournals.org

 The sequence was analyzed in an ABI PRISM 310 automated sequencer (PE Applied Biosystems).
Spoligotyping. To determine the species of Mycobacterium within the M. tuberculosis complex,
the spoligotyping system (Isogen) was applied in the Jerusalem laboratory. 

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2000

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Characterization of Mycobacterium tuberculosis isolates from patients in Houston, Texas, byspoligotyping

[HTML] from nih.gov

H Soini, X Pan, A Amin, EA Graviss… – Journal of clinical …, 2000 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The  square analysis was used to test the association of clustered spoligotypes with IS6110  The
sensitivity and specificity of spoligotyping were calculated by a method described 

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Use of spoligotype analysis to detect laboratory cross-contamination

B Nivin, J Driscoll, T Glaser, P Bifani… – Infection control and …, 2000 – JSTOR

 The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience
BV  In several cases of identical spoligotypes, the Wadsworth Center indicated that the 
Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP 

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Differentiation among Members of the Mycobacterium tuberculosis Complex by Molecular and Biochemical Features: Evidence for Two Pyrazinamide-Susceptible …

[HTML] from nih.gov

S Niemann, E Richter… – Journal of clinical …, 2000 – Am Soc Microbiol

 sequences which were bound on a nylon membrane by reverse line blotting (Isogen, Maarssen,
The  PZA, and source of isolates.All isolates have been analyzed by spoligotyping and were 
comprising at least two isolates could be found (Fig.1). The spoligotypes among isolates 

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Drug resistance rates of Mycobacterium tuberculosis strains in Austria between 1995 and 1998 and molecular typing of multidrug-resistant isolates

F Stauffer, A Makristathis, JP Klein… – Epidemiology and …, 2000 – Cambridge Univ Press

 se- quences of M. tuberculosis H37Rv and M. bo is BCG P3, being immobilized on a membrane
delivered by Isogen Bioscience (Maarssen  1. Spoligotyping of MDR MTB strains isolated be-
tween 1995 and 1998 in Austria: Lanes 1 and 19 represent the spoligotypes of the 

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Principe et intéręt du typage moléculaire appliqué a Mycobacterium tuberculosis

V Vincent… – Revue Francaise des Laboratoires, 2000 – Elsevier

 La methode 26 spoligotyping repose sur la detection du polymorphisme des ces sequences
non conservees.  douteux (figure .2). La realisation de la methode est facilitee par la
commercialisation de membranes portent l’ensemble de ces 43 sequences (Isogen Bioscience 

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1999

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Usefulness of Spoligotyping To Discriminate IS6110Low-Copy-Number Mycobacterium tuberculosis Complex Strains Cultured in Denmark

[HTML] from nih.gov

J Bauer, ÅB Andersen, K Kremer… – Journal of clinical …, 1999 – Am Soc Microbiol

 culture stock) with glass beads by vortexing (26). Membranes for spoligotyping were
obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. The membranes
contained oligonucleotides derived from the spacer DNA 

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DNA typing of a nonviable culture of Mycobacterium tuberculosis in a homeless shelter outbreak

[HTML] from nih.gov

JR Driscoll, MA McGarry… – Journal of clinical …, 1999 – Am Soc Microbiol

 Spoligotyping was performed on all the 1997 isolates from individuals involved in the outbreak,
and from a sample of nonviable cells from the 1992 culture of patient A, by using a commercially
available spoligotyping kit (Isogen, Maarssen, The Netherlands). 

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Comparative Evaluation of Ligation-Mediated PCR and Spoligotyping as Screening Methods for Genotyping ofMycobacterium tuberculosis Strains

[HTML] from nih.gov

S Bonora, MC Gutierrez, G Di Perri… – Journal of clinical …, 1999 – Am Soc Microbiol

 (i) Spoligotyping.Spoligotyping was performed  probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV  The software-assisted
evaluation of spoligotypes was done with GelCompar software, version 3.1b (Applied Maths 

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Molecular fingerprinting confirms extensive cow-to-cow intra-herd transmission of a single Mycobacterium bovis strain

VS Perumaalla, LG Adams, J Payeur, D Baca… – Veterinary …, 1999 – Elsevier

 Spoligotyping was performed according to protocol described by the manufacturer (Isogen
Bioscience BV, Maarssen, The Netherlands). The method is based on amplification of the
polymorphic DR locus that is specific to organisms in the M. tuberculosis complex. 

Cited by 14 – Related articles – All 7 versions – Import into BibTeX

DNA typing of Mycobacterium bovis strains from the Castlepoint area of the Wairarapa

DM Collins – New Zealand Veterinary Journal, 1999 – Taylor & Francis

 For spoligotyping, filters containing the appropriate array of primers (Isogen Bioscience, The
Netherlands  24 restriction types were differenti- ated into 11 PGRS types and 7 spoligotypes.
Combining the results of both PGRS typing and spoligotyping, dif- ferentiated the isolates 

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Extensive Cross-Contamination of Specimens withMycobacterium tuberculosis in a Reference Laboratory

[HTML] from nih.gov

MC Ramos, H Soini, GC Roscanni… – Journal of clinical …, 1999 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands  obtained between 8 May and 18 July 1995 were characterized by spoligotyping
and IS6110  Seventeen different spoligotypes were identified among the 91M. 

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Transmission dynamics of tuberculosis in a high-incidence country: prospective analysis by PCR DNA fingerprinting

[HTML] from nih.gov

WH Haas, G Engelmann, B Amthor… – Journal of clinical …, 1999 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed as previously described by Kamerbeek et al. (10). 
A total of 20 μl of the amplification product was hybridized with 43 oligonucleotides covalently
bound to a membrane (Isogen Bioscience BV  Analysis of spoligotypes was done by eye. 

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Evolution and clonal traits of Mycobacterium tuberculosis complex in Guinea-Bissau

[HTML] from nih.gov

G Källenius, T Koivula… – Journal of clinical …, 1999 – Am Soc Microbiol

 synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience
BV  of copies of IS6110 elements, and frequently lack the spoligotyping spacers 33  M.
tuberculosisisolates in terms of biochemical traits, RFLP patterns, and spoligotypes when they 

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Molecular Markers Demonstrate that the First Described Multidrug-Resistant Mycobacterium bovisOutbreak Was Due to Mycobacterium tuberculosis

[HTML] from nih.gov

MC Gutierrez, JC Galan, J Blazquez… – Journal of clinical …, 1999 – Am Soc Microbiol

 (v) Characterization of the DR region by spoligotyping.The DR cluster DNA of the strains was
amplified by PCR. The labeled PCR product was used as a probe to hybridize with 43 synthetic
spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV), as 

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[PDF] The Molecular Epidemiology of Tuberculosis in Montreal and British Columbia

[PDF] from nlc-bnc.ca

KA Musana – 1999 – nlc-bnc.ca

 50 5.2.2 Results of Spoligotyping ….. 50  49 Table 7 . Surnmary of the results of spoligotyping
of isolates found to be identical but with 5 or less IS6110 bands ….. 51 

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MOLECULAR FINGERPRINTING CONFIRMS EXTENSIVE COW-TO-COW INTRA-HERD TRANSMISSION OF A SINGLE MYCOBACTERIUM BOVIS STRAIN

VS Perumaalla, LG Adams, J Payeur, D Baca… – Veterinary …, 1999 – Elsevier

 Spoligotyping was performed according to protocol described by the manufacturer (Isogen
Bioscience BV, Maarssen, The Netherlands). The method is based on amplification of the
polymorphic DR locus that is specific to organisms in the M. tuberculosis complex. 

Cited by 14 – Related articles – All 7 versions – Import into BibTeX

DNA TYPING OF MYCOBACTERIUM BOVIS STRAINS FROM THE CASTLEPOINT AREA OF THE WAIRARAPA

DM Collins – New Zealand Veterinary Journal, 1999 – Taylor & Francis

 For spoligotyping, filters containing the appropriate array of primers (Isogen Bioscience, The
Netherlands  24 restriction types were differenti- ated into 11 PGRS types and 7 spoligotypes.
Combining the results of both PGRS typing and spoligotyping, dif- ferentiated the isolates 

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MOLECULAR CLUES OF A MICROEPIDEMY AMONG HOMELESS TUBERCULOSIS PATIENTS IN BUDAPEST DUE TO A NEW AND LOCAL MYCOBACTERIUM TUBERCULOSIS CLADE

C Ködmön, S Niemann, MC Gutierrez… – Infection, Genetics and …, 2007 – Elsevier

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands) according to the instructions of the manufacturer
(Kremer et al., 1999). Briefly, genomic mycobacterial DNA 

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TRANSMISSION DYNAMICS OF TUBERCULOSIS IN A HIGH-INCIDENCE COUNTRY: PROSPECTIVE ANALYSIS BY PCR DNA FINGERPRINTING

[HTML] from nih.gov

WH Haas, G Engelmann, B Amthor… – Journal of clinical …, 1999 – Am Soc Microbiol

 Spoligotyping.Spoligotyping was performed as previously described by Kamerbeek et al. (10). 
A total of 20 μl of the amplification product was hybridized with 43 oligonucleotides covalently
bound to a membrane (Isogen Bioscience BV  Analysis of spoligotypes was done by eye. 

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MYCOBACTERIUM TUBERCULOSIS GENOTYPES AND THEIR RELATIONSHIPS WITH CLINICAL AND IMMUNOLOGICAL PHENOTYPES IN SINGAPORE

[PDF] from 137.132.14.55

SUN YONGJIANG – 2006 – 137.132.14.55

 Furthermore, this method is highly reproducible and easy to perform (Kremer et al., 1999);
particularly spoligotypes can be digitized into binary or octal code formats (Dale et al., 2001)
which greatly facilitate the management and interlaboratory comparison of spoligotyping data. 

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EVOLUTION AND CLONAL TRAITS OF MYCOBACTERIUM TUBERCULOSIS COMPLEX IN GUINEA-BISSAU

[HTML] from nih.gov

G Källenius, T Koivula… – Journal of clinical …, 1999 – Am Soc Microbiol

 synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience
BV  of copies of IS6110 elements, and frequently lack the spoligotyping spacers 33  M.
tuberculosisisolates in terms of biochemical traits, RFLP patterns, and spoligotypes when they 

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MOLECULAR MARKERS DEMONSTRATE THAT THE FIRST DESCRIBED MULTIDRUG-RESISTANT MYCOBACTERIUM BOVISOUTBREAK WAS DUE TO MYCOBACTERIUM TUBERCULOSIS

[HTML] from nih.gov

MC Gutierrez, JC Galan, J Blazquez… – Journal of clinical …, 1999 – Am Soc Microbiol

 (v) Characterization of the DR region by spoligotyping.The DR cluster DNA of the strains was
amplified by PCR. The labeled PCR product was used as a probe to hybridize with 43 synthetic
spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV), as 

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[PDF] THE MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS IN MONTREAL AND BRITISH COLUMBIA

[PDF] from nlc-bnc.ca

KA Musana – 1999 – nlc-bnc.ca

 50 5.2.2 Results of Spoligotyping ….. 50  49 Table 7 . Surnmary of the results of spoligotyping
of isolates found to be identical but with 5 or less IS6110 bands ….. 51 

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USEFULNESS OF SPOLIGOTYPING TO DISCRIMINATE IS6110LOW-COPY-NUMBER MYCOBACTERIUM TUBERCULOSIS COMPLEX STRAINS CULTURED IN DENMARK

[HTML] from nih.gov

J Bauer, ÅB Andersen, K Kremer… – Journal of clinical …, 1999 – Am Soc Microbiol

 culture stock) with glass beads by vortexing (26). Membranes for spoligotyping were
obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. The membranes
contained oligonucleotides derived from the spacer DNA 

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DNA TYPING OF A NONVIABLE CULTURE OF MYCOBACTERIUM TUBERCULOSIS IN A HOMELESS SHELTER OUTBREAK

[HTML] from nih.gov

JR Driscoll, MA McGarry… – Journal of clinical …, 1999 – Am Soc Microbiol

 Spoligotyping was performed on all the 1997 isolates from individuals involved in the outbreak,
and from a sample of nonviable cells from the 1992 culture of patient A, by using a commercially
available spoligotyping kit (Isogen, Maarssen, The Netherlands). 

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COMPARATIVE EVALUATION OF LIGATION-MEDIATED PCR AND SPOLIGOTYPING AS SCREENING METHODS FOR GENOTYPING OFMYCOBACTERIUM TUBERCULOSIS STRAINS

[HTML] from nih.gov

S Bonora, MC Gutierrez, G Di Perri… – Journal of clinical …, 1999 – Am Soc Microbiol

 (i) Spoligotyping.Spoligotyping was performed  probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV  The software-assisted
evaluation of spoligotypes was done with GelCompar software, version 3.1b (Applied Maths 

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EXTENSIVE CROSS-CONTAMINATION OF SPECIMENS WITHMYCOBACTERIUM TUBERCULOSIS IN A REFERENCE LABORATORY

[HTML] from nih.gov

MC Ramos, H Soini, GC Roscanni… – Journal of clinical …, 1999 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands  obtained between 8 May and 18 July 1995 were characterized by spoligotyping
and IS6110  Seventeen different spoligotypes were identified among the 91M. 

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1998

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TOWARDS A STANDARDIZED APPROACH TO DNA FINGERPRINTING OF MYCOBACTERIUM BOVIS

DV Cousins, RA Skuce, RR Kazwala… – … of Tuberculosis and …, 1998 – ingentaconnect.com

 in word processing program Perform PGRS RFLP on common Spoligotypes – use Alu I digest 
Spoligotyping A standard set of oligonucleotides for amplification of spacer regions as  A
commercially available kit (Isogen, Bioscience BV, The Netherlands), containing spoligotyping 

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DRUG RESISTANCE RATES OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN AUSTRIA BETWEE

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2012

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Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria using a microarray system with automatic data processing and assignment

A Ruettger, J Nieter, A Skrypnyk… – Journal of Clinical …, 2012 – Am Soc Microbiol

 Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum
Biosolutions, Hydera- 79 bad, India) and the present DNA microarray.  ArrayStrip spoligotyping
and the assay’s capability of identifying new spoligotypes and li- 109 neages. 

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Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin

MI Millán-Lou, H Alonso, P Gavín… – Journal of Clinical …, 2012 – Am Soc Microbiol

 First, all 292 isolates were screened by spoligotyping with a commercially available
kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al.
(14). Seventy of the 292 isolates belonged to the Beijing family. 

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GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… – European …, 2012 – Eur Respiratory Soc

 Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the
Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer’s
instructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web 

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New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier

 Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotypingSpoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). 

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 [HTML] DISTRIBUTION OF SPOLIGOTYPING DEFINED GENOTYPIC LINEAGES AMONG DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES IN ANKARA, TURKEY

[HTML] from plos.org

O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… – PloS one, 2012 – dx.plos.org

 Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands  tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.
Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database 

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[HTML] SPOLIGOTYPING AND DRUG RESISTANCE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM NATIONAL SURVEY IN CHINA

[HTML] from plos.org

Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… – PloS one, 2012 – dx.plos.org

 A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as
described by  of the direct repeat region of Mycobacterium tuberculosis: implications for
interpretation of spoligotyping data.  2004) Predominant tuberculosis spoligotypes, Delhi, India. 

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[HTML] SPOLIGOTYPING AND VARIABLE NUMBER TANDEM REPEAT ANALYSIS OF MYCOBACTERIUM BOVIS ISOLATES FROM CATTLE IN BRAZIL

[HTML] from scielo.br

PM Parreiras, GI Andrade… – Memórias do Instituto …, 2012 – SciELO Brasil

 The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43
oligonucleotides of known spacer sequences by reversed-line blot hybridisation.  Spoligotyping –
Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 

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EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI

V Vadwai, A Shetty, P Supply… – Tuberculosis, 2012 – Elsevier

 Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the  13/69,
18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at  Analysis
of the congruence between MIRU-VNTR and spoligotyping results combined with the 

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EPIDEMIOLOGICAL SIGNIFICANCE OF THE DOMESTIC BLACK PIG (SUS SCROFA) IN THE MAINTENANCE OF BOVINE TUBERCULOSIS IN SICILY

V Di Marco, P Mazzone, MT Capucchio… – Journal of Clinical …, 2012 – Am Soc Microbiol

 direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 
M. bovis isolates from both field studies were genotyped by both spoligotyping 342  Four
spoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 

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TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS

E Augustynowicz-Kopeć, T Jagielski, M Kozińska… – Journal of Infection, 2012 – Elsevier

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen 
had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU  Assuming
isolates revealing both identical spoligotypes and VNTR profiles are representing the 

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[PDF] PREVALENCE OF PULMONARY TB AND SPOLIGOTYPE PATTERN OF MYCOBACTERIUM TUBERCULOSIS AMONG TB SUSPECTS IN A RURAL COMMUNITY IN SOUTHWEST ETHIOPIA

[PDF] from biomedcentral.com

A Deribew, G Abebe, L Apers… – BMC Infectious …, 2012 – biomedcentral.com

 Standard spoligotyping method [12] was done generally as described by Kamerbeek and
colleagues using a commercially available kit (Isogen Bioscience BV  Spoligotypes described
only once (non-clustered) in this study and in the SpolDB4 were designated as 

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CHARACTERIZING< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM KARACHI, PAKISTAN: DRUG RESISTANCE AND GENOTYPES

A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… – International Journal of …, 2012 – Elsevier

 The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the
World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18.  3.4. Genotypic
pattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped

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IMPLICATION OF THE RD< SUP> RIO</SUP>< I> MYCOBACTERIUM TUBERCULOSIS</I> SUBLINEAGE IN MULTIDRUG RESISTANT TUBERCULOSIS IN PORTUGAL

S David, EL Duarte, CQF Leite, JN Ribeiro… – Infection, Genetics and …, 2012 – Elsevier

 patient), were genotyped by spoligotyping according to the manufacturer’s instructions (Isogen
Bioscience BV  Contribution of Spoligotyping to the Characterization of the population structure
of  Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the 

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[PDF] ÇUKUROVA BÖLGESINDE AKCIĞER TÜBERKÜLOZLU HASTALARDAN İZOLE EDILEN MYCOBACTERIUM TUBERCULOSIS SUŞLARININ SPOLIGOTIPLENDIRME VE MIRU-VNTR YÖNTEMIYLE …

[PDF] from mikrobiyolbul.org

ÜO ZEYTİNLİ… – Mikrobiyol Bul, 2012 – mikrobiyolbul.org

 Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology;
Turkey. GİRİŞ  Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri
(Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. 

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2011

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[HTML] Bovine tuberculosis in a Nebraska herd of farmed elk and fallow deer: a failure of the tuberculin skin test and opportunities for serodiagnosis

[HTML] from hindawi.com

W Waters, GE Stevens, MA Schoenbaum… – Veterinary medicine …, 2011 – hindawi.com

 Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleic
acid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (Ocimum
Biosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. 

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 [HTML] The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines …

[HTML] from biomedcentral.com

E Abadia, J Zhang, V Ritacco… – BMC infectious …, 2011 – biomedcentral.com

 of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluate
the benefits of a new platform regarding the classical one on these samples. Most centers had
performed spoligotyping using commercial membranes (Ocimum, Hyderabad, India 

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Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterium reference laboratory

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer

 for spoli- gotyping results, standardization and reproducibility depend on the quality of the
commercial spoligotyping kit (Ocimum).  Spoligotyping Pattern  Both PETRA (a MIRU-VNTR lineage
with central asian spoligotypes, CAS) and Beijing clusters have been observed in other 

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DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …

S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier

 commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now Ocimum
Biosolutions) and  Owing to small sample size, the exact prevalence of various 272 spoligotypes
and M  Out of the isolates clustered by spoligotyping, all 280 were unique strains by 

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New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier

 Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotypingSpoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). 

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High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced immunodeficiency: implications for TB screening

[HTML] from nih.gov

T Oni, R Burke, R Tsekela, N Bangani, R Seldon… – Thorax, 2011 – thorax.highwire.org

 Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotyping
as previously described4 using a commercially available kit from Ocimum Biosolutions
BV, The Netherlands. The hybridisation patterns obtained 

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[HTML] Pathology of camel tuberculosis and molecular characterization of its causative agents in pastoral regions of Ethiopia

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G Mamo, G Bayleyegn, TS Tessema, M Legesse… – PloS one, 2011 – dx.plos.org

 SpoligotypingSpoligotyping was performed as previously described by Kamerbeek
and coauthors [22] and according to the spoligotype kit supplier’s instructions (Ocimum
Biosolutions Company, Iisselstein, The Netherlands). 

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POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA

Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… – Tuberculosis, 2011 – Elsevier

 Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The
Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the
SpoligDB4 database and assigned a spoligotyping international type (SIT). 

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[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED

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R Groenheit, S Ghebremichael, J Svensson… – PLoS One, 2011 – dx.plos.org

 All isolates that were subjected to RFLP were also spoligotyped Altogether, 414 isolates were
available for spoligotyping, of which 336 isolates were grouped into 42 clusters  Out of 229 isolates,
82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into 

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GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA

[PDF] from ajol.info

J Bazira, M Matte, BB Asiimwe… – African health sciences, 2011 – ajol.info

 was performed as previously described29 using a commercially available kit (Isogen Bioscience
BV  Spoligotypes that did not match any existing pattern in the SpolDB4 database were  tuberculosis
within the Mbarara, we have used RD analysis and spoligotyping to characterize 

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FLUOROQUINOLONE RESISTANCE AMONG MYCOBACTERIUM TUBERCULOSIS STRAINS FROM KARACHI, PAKISTAN: DATA FROM COMMUNITY-BASED FIELD CLINICS

[HTML] from nih.gov

Y Rafiq, K Jabeen, R Hasan, S Jafri… – Antimicrobial agents …, 2011 – Am Soc Microbiol

 was performed on all FQ-resistant strains using a commercially available kit provided by Isogen
Life Science BV  The spoligotypes were compared with the most prevalent M. tuberculosis
subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of 

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[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN

[HTML] from biomedcentral.com

YC Chiu, SF Huang, KW Yu, YC Lee… – BMC infectious …, 2011 – biomedcentral.com

 Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen
Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as deletion from spacer
1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. 

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[HTML] INITIAL PRESENTATIONS PREDICT MORTALITY IN PULMONARY TUBERCULOSIS PATIENTS-A PROSPECTIVE OBSERVATIONAL STUDY

[HTML] from plos.org

JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… – PloS one, 2011 – dx.plos.org

 All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience
BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. 

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[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA

[HTML] from nih.gov

X Gonzalo, M Ambroggi, E Cordova… – Emerging Infectious …, 2011 – ncbi.nlm.nih.gov

 DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according
to the manufacturer’s instructions (Isogen Life Science, IJsselstein, the Netherlands); and data
were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). 

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CHARACTERISTICS OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN TAIWAN: A POPULATION-BASED STUDY

CW Chang, MH Wu, PC Chuang… – Infection, Genetics and Evolution, 2011 – Elsevier

 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15
Netherlands) was used as  Even through spoligotyping results 3 revealed the genetic diversities
of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not 

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MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF BOVINE …

[PDF] from uaemex.mx

IF Padilla-Campos, JC Segura-Correa… – Tropical and …, 2011 – redalyc.uaemex.mx

 Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors.  Spoligotyping was carried
out following the protocol described by Kamerbeek et al  sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The 

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[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE

[HTML] from biomedcentral.com

C Gortazar, MJ Torres, P Acevedo, J Aznar… – BMC …, 2011 – biomedcentral.com

 Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified
product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). 

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MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF …

[PDF] from uady.mx

IF Padilla, JC Segura, JC Rodriguez… – Tropical and …, 2011 – ccba.uady.mx

 Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors.  Spoligotyping was carried
out following the protocol described by Kamerbeek et al  sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The 

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EPIDEMIOLOGICAL TRENDS AND CLINICAL COMPARISONS OF< I> MYCOBACTERIUM</I>< I> TUBERCULOSIS</I> LINEAGES IN THAI TB MENINGITIS

K Faksri, F Drobniewski, V Nikolayevskyy, T Brown… – Tuberculosis, 2011 – Elsevier

 isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns
were available) and spoligotyping results (141 spoligotypes were available  27 Spoligotyping
was performed according to the manufacturer’s instructions (Isogen Bioscience BV 

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A NOVEL METHOD FOR THE RAPID AND PROSPECTIVE IDENTIFICATION OF BEIJING MYCOBACTERIUM TUBERCULOSIS STRAINS BY HIGH‐RESOLUTION MELTING ANALYSIS

M Alonso, Y Navarro, F Barletta… – Clinical …, 2011 – Wiley Online Library

 Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of
the non-Beijing genotype, according to their spoligotypes) were grown on  Spoligotyping was
performed in accordance with the manufacturer’s instructions (Isogen, De Meern, the 

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QUALITY ASSURANCE FOR MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS METHODS IN THE MYCOBACTERIUM REFERENCE LABORATORY

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer

 43 spacer spoligotyping was performed according to the manufacturer’s instructions
(Isogen/Ocimun) as described previously.  27 Spoligotyping Pattern  Both PETRA (a MIRU-VNTR
lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other 

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EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLAND

M Krawczyk, A Brzostek, A Gorna… – … of Tuberculosis and …, 2011 – ingentaconnect.com

 of van Embden et al.16 Spoligotyping was performed as described by Kamerbeek et al., using
the Isogen Life Science kit  Spoligotyping analysis showed that 184 (78.6%) of the strains were
grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes

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ONE YEAR NATIONWIDE EVALUATION OF 24-LOCUS MIRU-VNTR GENOTYPING ON SLOVENIAN< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES

U Bidovec-Stojkovic, M Zolnir-Dovc… – Respiratory Medicine, 2011 – Elsevier

 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV 
identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping
jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only 

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MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) ISOLATED FROM CATTLE IN NORTHEAST AND NORTHWEST CHINA

Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… – Research in veterinary …, 2011 – Elsevier

 2.5. SpoligotypingSpoligotyping kits and Mini-blotter MN45 were obtained from Isogen
Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was
produced by Roche Applied Science Co. (Penzberg, Germany). 

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[HTML] TRANSMISSION PATTERN OF DRUG-RESISTANT TUBERCULOSIS AND ITS IMPLICATION FOR TUBERCULOSIS CONTROL IN EASTERN RURAL CHINA

[HTML] from plos.org

Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… – PloS one, 2011 – dx.plos.org

 Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience
BV  b Probaiblity that the Spoligotyping pattern belongs to the clades.  N, Bennett KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

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SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA

US Rajapaksa… – Indian Journal of Microbiology, 2011 – Springer

 Spoligotyping was carried out according to manufacturer’s guidelines using a
commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-
lates that confirmed to be Beijing strain were further characterised. 

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DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …

S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier

 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences ,
BV  Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.
tuberculosis  Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 

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MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA

AO Jenkins, SIB Cadmus, EH Venter… – Veterinary …, 2011 – Elsevier

 Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and
13 M  None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were 
All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical 

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GENETIC DIVERSITY OF THE MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY BASED ON IS6110, SNP, LSP AND VNTR PROFILES FROM THAILAND

K Faksri, F Drobniewski, V Nikolayevskyy… – Infection, Genetics and …, 2011 – Elsevier

 Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns were
available) and spoligotyping results (all 80 spoligotypes were available  Spoligotyping was
performed according to the manufacturer s instructions (Isogen Bioscience BV 

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[PDF] 骨结核临床分离菌株基因分型的特点

[PDF] from clinicmed.net

李文胜, 刘毅, 孙勇, 田苗, 刘忠泉… – 中华临床医师杂志 ( …, 2011 – clinicmed.net

 TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaKaRa 公司。
凝胶成像仪购自FOTO DYNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN45为荷兰
Isogen Bioscience 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 

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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES

[PDF] from qmul.ac.uk

A Saedi… – 2011 – qmro.qmul.ac.uk

 130 3.2.11 Identification of the UAE isolates by spoligotyping….. 132 3.2.12 Comparison between
MIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ….. 

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[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA

[HTML] from hindawi.com

S Barandiaran, EV Moras, A Adri& – Veterinary Medicine …, 2011 – hindawi.com

 Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun 
Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment 
isolates were defined as two or more M. bovis strains with identical spoligotypes

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[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL

[HTML] from scielo.br

SS Miranda, WS Carvalho, PN Suffys… – Memórias do Instituto …, 2011 – SciELO Brasil

 was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using
a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen
Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary 

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EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINA

Z Sun, R Cao, M Tian, X Zhang, X Zhang… – Research in Veterinary …, 2011 – Elsevier

 Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci
(ETR  Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following
the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV 

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[HTML] MYCOBACTERIUM TUBERCULOSIS SPOLIGOTYPES AND DRUG SUSCEPTIBILITY PATTERN OF ISOLATES FROM TUBERCULOSIS PATIENTS IN SOUTH-WESTERN UGANDA

[HTML] from biomedcentral.com

J Bazira, BB Asiimwe, ML Joloba… – BMC infectious …, 2011 – biomedcentral.com

 Spoligotyping. Standard spoligotyping was performed as previously described [29] using a
commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands).  Spoligotyping.
All the isolates had spoligotypes characteristic of M. tuberculosis. 

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A FIRST INSIGHT ON THE POPULATION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS COMPLEX AS STUDIED BYSPOLIGOTYPING AND MIRU-VNTRS IN BOGOTÁ, COLOMBIA

I Cerezo, Y Jiménez, J Hernandez, T Zozio… – Infection, Genetics and …, 2011 – Elsevier

 Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the 
Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes
and MIRU  was used to build minimum spanning trees based on spoligotyping and MIRU 

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[PDF] PRELIMINARY MAPPING STUDY OF MYCOBACTERIUM TUBERCULOSIS’ COMPLEX DNA BYSPOLIGOTYPING ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT …

[PDF] from digitaljournals.org

V Lisdawati, I Parwati… – Bulletin of Health …, 2011 – indonesia.digitaljournals.org

 PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian
pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen
Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. 

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[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS

[PDF] from uclm.es

A Balseiro, O Rodríguez, P González-Quirós… – prevalence, 2011 – uclm.es

 al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR
products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by
spoligotyping 127 (Kamerbeek et al., 1997). Results were 

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CLINICAL AND MICROBIOLOGICAL CHARACTERISTICS OF URINE CULTURE-CONFIRMED GENITOURINARY TUBERCULOSIS AT MEDICAL CENTERS IN TAIWAN FROM 1995 TO 2007

HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… – European journal of …, 2011 – Springer

 commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as described by the manufacturer. The fourth international spoligotyping database
(SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 

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MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATION

MV Afanas’ ev, LN Ikryannikova, EN Il’ina… – European Journal of …, 2011 – Springer

 spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur
J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered
in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. 

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ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS

[HTML] from ersj.org.uk

WJ Su, JY Feng, YC Chiu… – European Respiratory …, 2011 – Eur Respiratory Soc

 All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV,
Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least three of) spacers 35–43. 

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[PDF] A TWO-STEP STRATEGY FOR MOLECULAR TYPING OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM POLAND.

[PDF] from microbiology.pl

T JAGIELSKI, EWAA KOPEĆ… – Polish journal of …, 2011 – pjm.microbiology.pl

 Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV  e isolates
whose spoligotype patterns were already recorded in the database, were assigned “shared types”,
whereas those of spoligotypes identified for the first time, were 

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MOLECULAR TECHNOLOGIES USED IN DETECTING OF SENSITIVE AND ISONIAZID-RESISTANT MYCOBACTERIUM TUBERCULOSIS

OY Limanskaya… – Cytology and Genetics, 2011 – Springer

 The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian
Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl
(pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA 

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STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE

[HTML] from nih.gov

FS Spies, AW Ribeiro, DF Ramos… – Journal of clinical …, 2011 – Am Soc Microbiol

 Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV,
Netherlands  it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non 
Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since 

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GENOTYPING AND DRUG RESISTANCE PATTERNS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN FIVE PROVINCES OF CHINA

YL Guo, Y Liu, SM Wang, CY Li… – … of Tuberculosis and …, 2011 – ingentaconnect.com

 Of the (Isogen Bioscience BV, De Meern, The Netherlands).  1–1800 = shared spoligotypes. Table
3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number
IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU 

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ASSOCIATION OF< I> MYCOBACTERIUM TUBERCULOSIS</I> GENOTYPES AND CLINICAL AND EPIDEMIOLOGICAL FEATURES-A MULTI-CENTER STUDY IN TAIWAN

SF Huang, WJ Su, HY Dou, JY Feng… – Infection, Genetics and …, 2011 – Elsevier

 Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek
et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were
identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT 

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THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS

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LI Wen-sheng, LIU Yi, SUN Yong… – Chinese Journal of …, 2011 – global.clinicmed.net

 TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaKaRa 公司。
凝胶成像仪购自 FOTO DYNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN45为荷兰
Isogen Bioscience 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 

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MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE

EES Figueiredo, DF Ramos, L Medeiros… – The Veterinary …, 2011 – Elsevier

 to the spoligo-membrane was performed according to the manufacturer’s instructions (Isogen
Life Sciences).  The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm
clonal relationships  Four spoligotypes were identified among the 12 M. bovis isolates. 

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DETERMINAÇÃO DO CUSTO BIOLÓGICO EM ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS COM MUTAÇÕES NOS GENES RPSL, RRS, GIDB, RPOB E KATG

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FS Spies – 2011 – lume.ufrgs.br

 restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e
unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- 
Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região 

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A MAJOR SUBGROUP OF BEIJING FAMILY MYCOBACTERIUM TUBERCULOSIS IS ASSOCIATED WITH MULTIDRUG RESISTANCE AND INCREASED TRANSMISSIBILITY

Y Hu, X Ma, EA Graviss, W Wang… – Epidemiology and …, 2011 – Cambridge Univ Press

 Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen 
0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243  Thirty-three
(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that 

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[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA

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SK Thomas, CC Iravatham, BH Moni, A Kumar… – PloS one, 2011 – dx.plos.org

 the help of commercially available line probe arrays (Isogen Biosciences BV  tuberculosis complex
genetic diversity: mining the fourth international spoligotyping database (SpolDB4  KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

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[HTML] TUBERCULOSIS IN SUDAN: A STUDY OF MYCOBACTERIUM TUBERCULOSIS STRAIN GENOTYPE AND SUSCEPTIBILITY TO ANTI-TUBERCULOSIS DRUGS

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GSS Eldin, I Fadl-Elmula, MS Ali… – BMC infectious …, 2011 – biomedcentral.com

 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The  Spoligotyping
provided a relatively simple and rapid means of describing the population of  improved
discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU 

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[PDF] MOLECULAR CHARACTERISATION OF MYCOBACTERIUM BOVIS ISOLATED FROM AFRICAN BUFFALOES (SYNCERUS CAFFER) IN HLUHLUWE-IMFOLOZI PARK IN KWAZULU-NATAL, SOUTH AFRICA

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TM Hlokwe, AO Jenkins, EM Streicher… – … Journal of Veterinary …, 2011 – scielo.org.za

 One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates 
Spoligotyping Spoligotyping was done according to a standardised international method
(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). 

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GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL

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A Rocha, AR Elias, LF Sobral, DF Soares… – Tuberculosis, 2011 – Elsevier

 using PCR and hybridization conditions on a commercially available membrane (Isogen
Bioscience BV  All isolates that yielded spoligotypes that were recognized as not previously
described patterns were submitted to at least one additional spoligotyping assay and 

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MOLECULAR AND EPIDEMIOLOGICAL CHARACTERIZATION OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES IN JOHANNESBURG, SOUTH AFRICA

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CK Mlambo – 2011 – wiredspace.wits.ac.za

 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and
corresponding spoligotyping-based information (octal code, SIT, and clade)…………
64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 

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USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATION

B Nivin, J Driscoll, T Glaser, P Bifani… – Infection control and …, 2000 – JSTOR

 The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience
BV  In several cases of identical spoligotypes, the Wadsworth Center indicated that the 
Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP 

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CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA

L Xing, R Liu, Q Li, Z Peng… – European Journal of Clinical …, 2011 – Springer

 Of 105 isolates spoligotyped, the major spoligotypes found were  Spoligotyping was performed
according to the internation- ally standardized protocol [5]. A commercially available spoligotyping
kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described 

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TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEP

M Muņoz Mendoza, L Juan, S Menéndez… – The Veterinary …, 2011 – Elsevier

 by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV).  Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and
M. bovis  close contact with cattle or goats infected by the same spoligotypes (Table 1 

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[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL

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SSS Higino, SR Pinheiro, GO Souza… – Brazilian Journal of …, 2011 – SciELO Brasil

 Isolate was characterised by spoligotyping as described (7). The amplified product
was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). 

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EPIDEMIOLOGICAL STUDIES OF BEIJING STRAINS OF MYCOBACTERIUM TUBERCULOSIS FROM TAIPEI AND OTHER ASIAN CITIES BASED ON MIRU PROFILES

JUNREN SUN, HYUN DOU, SYI LEE… – Apmis, 2011 – Wiley Online Library

 on isolates from areas where the Beijing strain is prevalent as most isolates in this family share
the same spoligotypes (1, 5  Spoligotyping  to the manufacturers’ protocol, to a set of 43 oligonucle-
otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit 

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2010

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Comparison of restriction fragment length polymorphism with the polymorphic guanine-cytosine-rich sequence and spoligotyping for differentiation of Mycobacterium …

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L Flores, LG Jarlsberg, EY Kim… – Journal of clinical …, 2010 – Am Soc Microbiol

 2004 was performed with standardized methods (15) using a membrane with 43 oligonucleotides
representing the different direct variable repeats (DVRs) immobilized in specific locations (Ocimum
Biosolutions, Inc., Gaithersburg, MD). Beginning in 2004, spoligotyping of the 

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Fitness study of the RDRio lineage and Latin American–Mediterranean family of Mycobacterium tuberculosis in the city of Rio Grande, Brazil

A Von Groll, A Martin, C Felix… – FEMS Immunology …, 2010 – Wiley Online Library

 RD Rio BrgR: 5′-CACCGCCACGCTGAATGAGACCA-3′, –. Genotyping by
spoligotypingSpoligotyping was performed with a commercial kit (Ocimum Biosolutions
BV, India) according to standard procedures (Kamerbeek et al., 1997). 

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[HTML] Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype

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A Von Groll, A Martin, M Stehr, M Singh, F Portaels… – PloS one, 2010 – dx.plos.org

 Strains were fully characterized and analyzed for the presence of mutations conferring resistance
to RMP and INH. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions BV,
India) according to standard procedures [29]. Molecular characterization. 

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[HTML] MOLECULAR DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PATIENTS WITH PULMONARY TUBERCULOSIS IN MOZAMBIQUE

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SO Viegas, A Machado, R Groenheit… – BMC …, 2010 – biomedcentral.com

 using a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands).
Spoligotyping results were analysed with the BioNumerics Software ver. 5.01 (Applied Maths,
Kortrijk, Belgium). Database comparison and geographical distribution of spoligotypes

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[PDF] ASSOCIATION AMONG THE RV2629 GENE, RPOB GENE, RFP RESISTANCE AND BEIJING GENOTYPE IN M. TUBERCULOSIS CLINICAL ISOLATES

[PDF] from academicjournals.org

J Zhang, X Wu, Y Yang, Y Zhuang… – African Journal of …, 2010 – academicjournals.org

 The amplified PCR products were hybridized with spoligotyping Biodyne C membrane immobilized
43 oligonucleotide probes (Isogen Life Science, Holland).  A total of 8 distinct spoligotyping patterns
were observed.  7 isolates had unique spoligotypes (Figure 1, Tables 1 and 2 

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SINGLE NUCLEOTIDE POLYMORPHISMS IN CELL WALL BIOSYNTHESIS-ASSOCIATED GENES AND PHYLOGENY OF< I> MYCOBACTERIUM TUBERCULOSIS</I> LINEAGES

PC Chuang, YMA Chen, HY Chen… – Infection, Genetics and …, 2010 – Elsevier

 2.2. Genotype analysis. Spoligotyping using a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands  Spoligotypes and RFLP patterns were scanned and analyzed
with Bionumerics ® software (version 4.601; Applied Maths, Kortrijk, Belgium). 

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[HTML] ASSOCIATIONS BETWEEN MYCOBACTERIUM TUBERCULOSIS STRAINS AND PHENOTYPES

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T Brown, V Nikolayevskyy, P Velji… – Emerging Infectious …, 2010 – ncbi.nlm.nih.gov

 Spoligotyping was performed according to the manufacturer’s instructions (Isogen Lifescience,
IJsselstein, the Netherlands) (9). Images were digitized and entered into BioNumerics software
by using the BNIMA module (Applied Maths). Spoligotypes were assigned to families 

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[HTML] SENSIBILIDAD Y ESPECIFICIDAD DE PCR ANIDADA Y SPOLIGOTYPING COMO PRUEBAS RÁPIDAS DE DIAGNÓSTICO DE TUBERCULOSIS BOVINA EN TEJIDO FRESCO

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F Milián Suazo, B Harris, C Arriaga Díaz… – Revista mexicana de …, 2010 – scielo.org.mx

 Spoligotyping se realizó tal como lo describe Kamerbeek et al (17) . De manera
resumida, la amplificación del  por 30 seg. La hibridización se realizó de acuerdo
a las recomendaciones del fabricante (Isogen). El ADN hibridado 

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[HTML] GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM ALOW-ENDEMIC SETTING IN NORTHWESTERN STATE OF PARANÁ IN SOUTHERN BRAZIL

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EN Noguti, CQF Leite, AC Malaspina… – Memórias do Instituto …, 2010 – SciELO Brasil

 Spoligotyping – Spoligotyping was performed in all 93 M. tuberculosis isolates, using the standard
method  of 43 spacer oligonucleotides covalently linked to the spoligo-membrane (Isogen Life
Sciences  Spoligotypes were reported using an octal code in which the 43-digit binary 

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[HTML] EXTENSIVE TRANSMISSION OF ISONIAZID RESISTANT M. TUBERCULOSIS AND ITS ASSOCIATION WITH INCREASED MULTIDRUG-RESISTANT TB IN TWO RURAL COUNTIES OF EASTERN CHINA: A …

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Y Hu, S Hoffner, W Jiang… – BMC infectious …, 2010 – biomedcentral.com

 Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV  Table 3.
Spoligotyping pattern and frequency of the MTB isolates included.  B, Kurepina N, Bennett KP:
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

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[HTML] MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES FROM ETHIOPIAN CATTLE

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D Biffa, E Skjerve, J Oloya, A Bogale… – BMC veterinary …, 2010 – biomedcentral.com

 spacers were then hybridized with the known oligonucleotide spacers ligated to the membrane
(Isogen).  The discriminatory power (HGDI) of the spoligotyping method was calculated to be 0.81.
Also on the basis of the above findings (the number of distinct spoligotypes (S) = 12 

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[HTML] MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN, BAMAKO, MALI

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B Diarra, S Siddiqui, D Sogoba… – Emerging infectious …, 2010 – ncbi.nlm.nih.gov

 See the article”Global Distribution of Mycobacterium tuberculosis Spoligotypes” in volume 8 on
page 1347.  Spoligotyping using a commercially available kit (Spoligotyping Isogen Life Science,
De Meern, the Netherlands) showed characteristics of the Beijing clade (Appendix 

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EVALUATION OF 24 LOCUS MIRU-VNTR GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN CANADA

S Christianson, J Wolfe, P Orr, J Karlowsky… – Tuberculosis, 2010 – Elsevier

 some cases. 22 Blots were purchased from Isogen Biosolutions and run according
to the manufacturer’s directions. Spoligotyping patterns were converted to the “octal”
pattern using the previously described formula. 23 Data 

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MYCOBACTERIUM MICROTI INFECTION ASSOCIATED WITH SPINDLE CELL PSEUDOTUMOUR AND HYPERCALCAEMIA: A POSSIBLE LINK WITH AN INFECTED ALPACA

C McGoldrick, C Coghlin, A Seagar… – BMJ Case …, 2010 – casereports.bmj.com

 At SMRL spoligotyping was performed using a commercial assay available from Isogen Life
Science (3600 BT; Maarssen, The Netherlands) and 15-locus Mycobacterial Interspersed
Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing, performed using the 

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MOLECULAR EPIDEMIOLOGY OF HUMAN CASES OF TUBERCULOSIS BY MYCOBACTERIUM BOVIS IN MEXICO

F Milián-Suazo, L Pérez-Guerrero… – Preventive veterinary …, 2010 – Elsevier

 Coding allows easy comparisons of spoligotypes from different laboratories and from different
parts of  Spoligotyping was performed following a standard protocol (Kemerbeek et al., 1997).  6
spacer sequences from M. bovis BCG were covalently bound (ISOGEN, Maarssen, the 

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THE MOST PREDOMINANT SPOLIGOPATTERNS OF< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES AMONG IRANIAN, AFGHAN-IMMIGRANT, PAKISTANI AND TURKISH TUBERCULOSIS PATIENTS: …

MA Merza, P Farnia, AM Salih, MR Masjedi… – …, 2010 – content.karger.com

 [19] , with a commercially available kit, according to the manufacturer’s instructions (Isogen Biosci-
ence, The  Analysis of Spoligotyping Data A total of 133 distinct spoligopatterns were identi- fied 
The distribution of spoligotypes (T, CAS, EAI, Haarlem, X, Beijing, Or- phan and 

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[HTML] CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING ISOLATES FROM THE MEDITERRANEAN AREA

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M Alonso, NA Rodriguez, C Garzelli… – BMC …, 2010 – biomedcentral.com

 deletion of the spacers 1-34 and the characteristic hybridization pattern of spacers 35-43, and
the remaining seven corresponded to variant “Beijing-like” spoligotypes Spoligotyping was
performed following the manufacturer’s instructions (Isogen, Netherlands). 

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SPOLIGOTYPE-BASED COMPARATIVE POPULATION STRUCTURE ANALYSIS OF MULTIDRUG-RESISTANT AND ISONIAZID-MONORESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES …

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T Jagielski, E Augustynowicz-Kopeć… – Journal of clinical …, 2010 – Am Soc Microbiol

 CTAB) method (47) were stored at −20°C before being subjected to spoligotyping using a 
hybridized to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science
BV  Spoligotypes in binary format were converted to an octal code for comparison with the 

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[HTML] FIRST INSIGHTS INTO THE GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM HIV-INFECTED MEXICAN PATIENTS AND MUTATIONS CAUSING MULTIDRUG RESISTANCE

[HTML] from biomedcentral.com

R Lopez-Alvarez, C Badillo-Lopez… – BMC …, 2010 – biomedcentral.com

 To determine lineage, the 57 strains (48 MTb and 9 M. bovis) from the MTC were spoligotyped
and binary outcomes were compared with the shared type (ST) number and lineages and
sublineages reported by  Spoligotyping Spoligotypes were reported using an octal code [74]. 

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MOLECULAR EVIDENCE OF FALSE-POSITIVE CULTURES FOR MYCOBACTERIUM TUBERCULOSIS IN A TAIWANESE HOSPITAL WITH A HIGH INCIDENCE OF TB

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CC Lai, CK Tan, SH Lin, CH Liao… – Chest, 2010 – chestjournal.chestpubs.org

 Spoligotyping was performed according to the internationally standardized protocol to identify
and differentiate  10 A commercially available kit (Isogen Bioscience BV; Maarssen, The
Netherlands) was  database was used to assign genotype families to the spoligotypes obtained 

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MOLECULAR DETECTION OF MIXED INFECTIONS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN SPUTUM SAMPLES FROM PATIENTS IN KARONGA DISTRICT, MALAWI

[HTML] from nih.gov

K Mallard, R McNerney, AC Crampin… – Journal of clinical …, 2010 – Am Soc Microbiol

 on these isolates with the aid of a commercially available kit (Isogen Biosciences BV  These isolates
were also spoligotyped and had previously been characterized by IS6110-RFLP  Spoligotyping
versus Beijing and LAM PCR results for 377 clinical M. tuberculosis isolates used 

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[HTML] CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS CASES IN ACAPULCO: SPOLIGOTYPING AND RISK FACTORS

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E Nava-Aguilera, E Harris, S Mitchell… – Clinical and …, 2010 – hindawi.com

 DNA with 43 oligonucleotides covalently linked to a nylon membrane (Isogen Bioscience BV 
SpolTools permitted the analysis of M. tuberculosis spoligotyping data to identify emerging  and
visualization of the probable evolutionary relationships between the spoligotypes in our 

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TRACING THE ORIGINS OF< I> MYCOBACTERIUM BOVIS</I> TUBERCULOSIS IN HUMANS IN THE USA TO CATTLE IN MEXICO USING SPOLIGOTYPING

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TC Rodwell, AJ Kapasi, M Moore… – International Journal of …, 2010 – Elsevier

 For this study, we also examined all available spoligotypes in the US national surveillance
database of M. bovis isolates sent to the National Veterinary Services Laboratories  Spoligotyping
was performed by all laboratories using the Spoligo Kit (Isogen Bioscience BV 

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HIGH SPOLIGOTYPE DIVERSITY WITHIN A< I> MYCOBACTERIUM BOVIS</I> POPULATION: CLUES TO UNDERSTANDING THE DEMOGRAPHY OF THE PATHOGEN IN EUROPE

[PDF] from tcd.ie

S Rodríguez, B Romero, J Bezos, L De Juan… – Veterinary …, 2010 – Elsevier

 The isolates were spoligotyped following the protocol described by Kamerbeek et al  Frequencies
of the 43 spacers of the DR locus that are included in the spoligotyping membrane (designed 
Data expressed as percentage of spoligotypes (n = 252) in which the spacer is present 

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MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE IS AN INDEPENDENT RISK FACTOR FOR TUBERCULOSIS TREATMENT FAILURE IN INDONESIA

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I Parwati, B Alisjahbana, L Apriani… – Journal of Infectious …, 2010 – jid.oxfordjournals.org

 of the Beijing genotype per se or to confounding by drug resistance, which has been linked to
the Beijing genotype in different settings [1]. We previously spoligotyped a large  Spoligotyping
was performed using a commercial kit (Isogen Bioscience) according to the 

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EFFECT OF SERIAL SUBCULTURING ON THE GENETIC COMPOSITION AND CYTOTOXIC ACTIVITY OF MYCOBACTERIUM TUBERCULOSIS

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CA Molina-Torres, J Castro-Garza… – Journal of medical …, 2010 – Soc General Microbiol

 (1997) was used. PCR amplification of the direct repeat locus was performed on heat-treated
cell suspensions. Biotin-labelled PCR products were detected by hybridization onto a
spoligotyping membrane (Isogen Bioscience). Microarray analysis. 

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[HTML] DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS GENOTYPES CIRCULATING IN NDOLA, ZAMBIA

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C Mulenga, I Shamputa, D Mwakazanga… – BMC infectious …, 2010 – biomedcentral.com

 Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according  Spoligotyping and MIRU-VNTR patterns were compared to the
international Spol DB4.0  Spoligotypes that were not present in the Spol DB4.0 are referred to 

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PHENOTYPIC AND GENOTYPIC ANALYSIS OF MULTIDRUG-RESISTANT TUBERCULOSIS IN ETHIOPIA

M Agonafir, E Lemma… – … of Tuberculosis and …, 2010 – ingentaconnect.com

 to the extraction protocol described by van Soolingen et al.14 Spoligotyping was performed  the
commercially available kit from Isogen Bioscience BV, Maarssen, The Netherlands, according
to  Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 

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[PDF] SENSIBILIDAD Y ESPECIFICIDAD DE PCR ANIDADA Y SPOLIGOTYPING COMO PRUEBAS RÁPIDAS DE DIAGNÓSTICO DE TUBERCULOSIS BOVINA EN TEJIDO FRESCO

[PDF] from scielo.org.mx

FM Suazoa, B Harrisb, CA Díazc… – Revista Mexicana de …, 2010 – scielo.org.mx

 Spoligotyping was performed essentially as described by Kamerbeek(17). Briefly,
amplification of DNA was run in  for 30 sec. Hybridization was performed as
recommended by the manufacturer (Isogen). Hybridized DNA was detected 

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CLINICAL AND GENOTYPIC CHARACTERISTICS OF EXTENSIVELY DRUG-RESISTANT AND MULTIDRUG-RESISTANT TUBERCULOSIS

CC Lai, CK Tan, SH Lin, CH Liao… – European journal of …, 2010 – Springer

 by serial dilution on agar plates as described previously [1]. Spoligotyping, according to  the M.
tuberculosis complex isolates [3]. A commercially available kit (Isogen Bioscience BV  1
Dendrograms of spoligotypes generated by clustered Myco- bacterium tuberculosis strains after 

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IDENTIFICATION OF WILD-TYPE MYCOBACTERIUM TUBERCULOSIS ISOLATES AND POINT MUTATIONS ASSOCIATED WITH ISONIAZID RESISTANCE

[PDF] from iekvm.kharkov.ua

OY Limanskaya, TN Mukhina, VN Stepanshina… – Molecular Biology, 2010 – Springer

 carrying AGC AGA; HB125, carrying AGC ACC, and H37Rv with the wild type codon
315 (negative control for amplification). The reaction mixture (20–50 μl) was prepared
from a dry amplification kit (Isogen, Russia). Its com 

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A SNAPSHOT OF THE BIODIVERSITY AND CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS IN OMAN USINGSPOLIGOTYPING

[DOC] from kit.nl

A Al-Maniri, JPN Singh, O Al-Rawas… – … of Tuberculosis and …, 2010 – ingentaconnect.com

 Figure Spoligotyped distribution of  Molecular typing of Mycobacterium tuberculosis isolates
from a rural area of Kanpur by spoligotyping and mycobacterial interspersed re- petitive units
(MIRUs) typing.  Predominant tubercu- losis spoligotypes, Delhi, India. 

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MYCOBACTERIUM TUBERCULOSIS COMPLEX CRISPR GENOTYPING: IMPROVING EFFICIENCY, THROUGHPUT AND DISCRIMINATIVE POWER OF ‘SPOLIGOTYPING’WITH NEW SPACERS AND A …

[HTML] from sgmjournals.org

J Zhang, E Abadia, G Refregier… – Journal of medical …, 2010 – Soc General Microbiol

 Thus, as spoligotypes are considered both as phylogenetic and epidemiological markers (Asiimwe
et al., 2008; Sola et al., 2001; Tanaka & Francis, 2006  Both sets had been previously genotyped
by spoligotyping using commercial membranes (Isogen Biosciences). 

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[HTML] MOLECULAR DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PATIENTS WITH TUBERCULOSIS IN HONDURAS

[HTML] from biomedcentral.com

S Rosales, L Pineda-García… – BMC …, 2010 – biomedcentral.com

 All isolates were spoligotyped in order to identify the predominant genotypes within this  SG
performed and analyzed RFLP; carried out bioinformatics analysis of spoligotyping and RFLP
results  NR performed database analysis of the spoligotypes and helped draft the manuscript 

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[HTML] PRODUCTION OF TNF-Α, IL-12 (P40) AND IL-17 CAN DISCRIMINATE BETWEEN ACTIVE TB DISEASE AND LATENT INFECTION IN A WEST AFRICAN COHORT

[HTML] from plos.org

JS Sutherland, BC de Jong, DJ Jeffries, IM Adetifa… – PloS one, 2010 – dx.plos.org

 10ng of DNA was used for spoligotype analysis with commercially available membranes (Isogen
Biosciences, The Netherlands).  Contributed reagents/materials/analysis tools: BCdJ. Wrote the
paper: JSS MOCO. Performed spoligotyping: BCdJ. Statistical analysis: DJJ. 

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[BOOK] RAPID TESTS FOR MULTIDRUG RESISTANT TUBERCULOSIS IN LOW INCOME SETTINGS

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F Bwanga – 2010 – mbl.mak.ac.ug

 In paper V, we applied spoligotyping to study the clustering rate and predominant genotypic strains
of 99 MDR-TB strains isolated from patients in Kampala. Eighty three percent of the strains
occurred in clusters, and the T2 lineage was the largest single cluster. Conclusion. 

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*************************************************************

2009

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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex

JR Driscoll – Methods Mol. Biol, 2009 – Springer

 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV  potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26).  spacer
sequences would improve the differentiability of the commonly observed spoligotypes

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Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol

 The fast DNA extraction method (amount used, 2 μl) was also successfully employed for
spoligotyping (Ocimum Biosolution Company, India).  In addition, further CAS MIRU-VNTR types
and associated spoligotypes were identified in the literature (3, 6, 7, 58, 61, 62). 

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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES (UAE)

[DOC] from qmul.ac.uk

A Saaidi… – 2009 – qmro.qmul.ac.uk

 HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 

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BEIJING GENOTYPE OF MYCOBACTERIUM TUBERCULOSIS IS SIGNIFICANTLY ASSOCIATED WITH HIGH-LEVEL FLUOROQUINOLONE RESISTANCE IN VIETNAM

[HTML] from nih.gov

DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol

 with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV  were assigned to the Euro-American clade on the basis of spoligotyping patterns 
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive 

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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

[PDF] from lrsitbrd.nic.in

TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in

Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 

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[HTML] SUPERVISED LEARNING FOR THE AUTOMATED TRANSCRIPTION OF SPACER CLASSIFICATION FROM SPOLIGOTYPE FILMS

[HTML] from biomedcentral.com

D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com

 a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen  Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a  AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of 

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POLYMORPHIC EXACT TANDEM REPEAT A (PETRA): A NEWLY DEFINED LINEAGE OF MYCOBACTERIUM TUBERCULOSIS IN ISRAEL ORIGINATING PREDOMINANTLY IN SUB-SAHARAN AFRICA

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol

 In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in 
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9  performed using a kit according to the manufacturer’s instructions (Isogen/Omicium 

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A RAPID AND SIMPLE METHOD FOR IDENTIFYING MYCOBACTERIUM TUBERCULOSIS W-BEIJING STRAINS BASED ON DETECTION OF A UNIQUE MUTATION IN RV0927C BY PCR-SSCP

X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier

 Spoligotyping was performed as previously described [16  in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for 

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Using a multiplex polymerase chain reaction for the identification of Beijing strains of Mycobacterium tuberculosis

JR Sun, SY Lee, HY Dou… – European Journal of Clinical …, 2009 – Springer

 Page 2. in the Isogen Bioscience spoligotyping kit (Isogen Life Science, the Netherlands).  Among
the 323 clinical MTB isolates, a total of 78 different spoligotypes were found. One hundred and
sixty-nine isolates were determined to belong to the Beijing family. 

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[HTML] Mycobacterium bovis en Argentina: aislamientos de gatos tipificados por spoligotyping

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MJ Zumárraga, M Martínez Vivot… – Revista argentina de …, 2009 – SciELO Argentina

 (8) , and using the spoligotyping kit (Isogen Biosolutions BV  Each of the different spoligotypes
were allocated a number.  H37Rv (ATCC 27294) and M. bovis Bacillus Calmette-Guerin (BCG)
(ATCC 27289) were included as reference strains in each spoligotyping experiment. 

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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex

JR Driscoll – Methods Mol. Biol, 2009 – Springer

 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV  potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26).  spacer
sequences would improve the differentiability of the commonly observed spoligotypes

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Use of spoligotyping and large sequence polymorphisms to study the population structure of the Mycobacterium tuberculosis complex in a cohort study of consecutive …

[HTML] from nih.gov

BC De Jong, M Antonio, T Awine… – Journal of clinical …, 2009 – Am Soc Microbiol

 After spectrophotometry, we used 10 ng of DNA for spoligotype analysis (16) with commercially
available membranes (Isogen Biosciences, Maarssen, The Netherlands  If spoligotype analysis
did not result in a clear pattern of spacers, the isolate underwent repeat spoligotyping

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Isoniazid and rifampicin resistance-associated mutations in Mycobacterium tuberculosis isolates from Yangon, Myanmar: implications for rapid molecular testing

[HTML] from oxfordjournals.org

H Valvatne, H Syre, M Kross… – Journal of …, 2009 – Br Soc Antimicrob Chemo

 identification test (GenProbe Incorporated, San Diego, CA, USA) and by spoligotyping (Isogen
Bioscience BV  A total of 43 different spoligotypes were observed among the 96 isoniazid-resistant 
of the 63 isolates that could not be differentiated by spoligotyping produced diverse 

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… of the Mycobacterium tuberculosis complex in Albania obtained by multilocus variable-number tandem-repeat analysis and spoligotyping reveals the presence of …

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S Tafaj, J Zhang, Y Hauck, C Pourcel… – Journal of clinical …, 2009 – Am Soc Microbiol

 ethambutol (2.0 μg/ml) (5). Identification at the species level was performed by phenotypic methods
and verified by a genotypic method on isolates with rare spoligotypes and/or  Spoligotyping was
performed using a commercial membrane (Isogen, The Netherlands 

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Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis

PICPI Cafrune, LGPLG Possuelo… – Canadian Journal …, 2009 – NRC Research Press

 was performed using a commercial kit according to the manufacturer’s instructions (Isogen
Biosciences BV  Table 1. Accuracy of spoligotyping results according to culture grading.  A total
of 44 different spoligotypes were identified; 112 isolates (83%) were included in 21 shared 

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Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variable-number tandem repeats for geographically optimized …

[HTML] from nih.gov

MB Boniotti, M Goria, D Loda… – Journal of clinical …, 2009 – Am Soc Microbiol

 repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV  into 64 different patterns, while the seven M. caprae spoligotypes were subdivided  The
discrimination provided by combination of spoligotyping and ETR methods allowed for a 

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Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates

HY Dou, JJ Lu, CW Lin, JR Chang, JR Sun… – Journal of microbiological …, 2009 – Elsevier

 Spoligotyping was carried out using a spoligotyping kit according to the manufacturer’s instructions
(Isogen Bioscience BV). Spoligotypes common to more than one strain were designated as shared
types (ST) and assigned a shared international type number (SIT) according to 

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[PDF] Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing

[PDF] from medind.nic.in

S Shenai, C Rodrigues, M Sadani, N Sukhadia… – Indian J Tuberc, 2009 – medind.nic.in

 All the PZA resistant isolates were further spoligotyped to identify M. bovis, if present.  Finger
printing – Spoligotyping All the 63 culture isolates were subjected to spoligotyping using a
commercially available Isogen kit, Netherlands as described by Kamerbeek et al.11 Briefly 

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Molecular analysis of< i> Mycobacterium tuberculosis</i> from tuberculous meningitis patients in Thailand

J Yorsangsukkamol, A Chaiprasert, T Prammananan… – Tuberculosis, 2009 – Elsevier

 2.4. SpoligotypingSpoligotyping was performed with a commercially available
kit (Isogen Bioscience BV, Maarsen, The Netherlands).  25 All obtained spoligotypes
were compared to SpolDB4 for analysis. 2.5. Data analysis. 

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Prevalence of ST26 among untreated smear-positive tuberculosis patients from Karachi indicating ongoing transmission

S Shakoor, M Tanveer, Y Rafiq… – Scandinavian …, 2009 – informahealthcare.com

 Five different resistance phenotypes were identified among the isolates spoligotyped: isolated
low-level isoniazid resistance (resistant to 0.2 µg/ml) in 1 isolate; high-level isoniazid resistance
(resistant to 1  Spoligotypes Spoligotyping grouped 66 isolates into 9 different clusters. 

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[PDF] Reinout van Crevel1, Ida Parwati2, Edhyana Sahiratmadja2, Sangkot Marzuki3, Tom HM Ottenhoff4, Mihai G. Netea1, Andre van der Ven1, Ronald H. Nelwan5, …

[PDF] from kun.nl

BA van der Meer… – Factors Underlying the Success … – repository.ubn.kun.nl

 min. Spoligotyping was performed using a commercial kit (Isogen Bioscience,
Maarssen, The Netherlands). The  min. Spoligotyping was performed using a
commercial kit (Isogen Bioscience, Maarssen, The Netherlands). The 

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Emergence of new forms of totally drug-resistant tuberculosis bacilli

[HTML] from chestpubs.org

AA Velayati, MR Masjedi, P Farnia… – Chest, 2009 – chestjournal.chestpubs.org

 by polymerase chain reaction (PCR) using primers derived from the direct repeat sequence
(Isogen Bioscience; Maarssen, the  and CAS (n = 4; 17.5%) [Table 2]. The VNTR profiles of
spoligotypes strains were  Spoligotyping Patterns and VNTR Profiles of XDR and TDR Patients. 

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[PDF] Mycobacterium tuberculosis Beijing genotype in Indonesia is associated with drug resistance gene mutations in katG, rpoB and embB

[PDF] from kun.nl

I Parwati, R van Crevel, J de Beer… – Factors Underlying the … – repository.ubn.kun.nl

 of rpoB-hotspot region Three hundred and twenty five spoligotyped isolates were  Mycobacterium
tuberculosis complex genetic diversity: mining the fourth international spoligotyping database
(SpolDB4  mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug 

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Infection with Mycobacterium tuberculosis Beijing genotype strains is associated with polymorphisms in SLC11A1/NRAMP1 in Indonesian patients with tuberculosis

[HTML] from oxfordjournals.org

R Van Crevel, I Parwati… – Journal of Infectious …, 2009 – jid.oxfordjournals.org

 Mycobacterial DNA was extracted by bringing 2 loops of bacterial mass from a M.
tuberculosis culture in saline and subsequently heating it at 95°C for 5 min.
Spoligotyping was performed using a commercial kit (Isogen Bioscience). 

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Unexpectedly high proportion of ancestral Manu genotype Mycobacterium tuberculosis strains cultured from tuberculosis patients in Egypt

[HTML] from nih.gov

ZH Helal, MSED Ashour, SA Eissa… – Journal of clinical …, 2009 – Am Soc Microbiol

 was amplified with primers DRa and DRb, and the amplified biotinylated products were hybridized
to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science BV 
Comparison of spoligotyping results with database types.Spoligotypes in binary 

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Differences in Cell Wall Thickness between Resistant and Nonresistant Strains of< i> Mycobacterium tuberculosis</i>: Using Transmission Electron Microscopy

[PDF] from mrc.ac.ir

AA Velayati, P Farnia, TA Ibrahim… – …, 2009 – content.karger.com

 For spoligotyping, the direct repeat region was am- plified by PCR using primers derived from
the direct repeat se- quence (Isogen Bioscience).  Spoligopatterns and VNTR Profiles When the
spoligotype patterns were compared with earlier published spoligotypes [5] , our 

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Mycobacterium tuberculosis genotypic diversity in pyrazinamide-resistant isolates of Iran

[PDF] from mrc.ac.ir

F Doustdar, AD Khosravi… – Microbial Drug …, 2009 – online.liebertpub.com

 Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarsen, The  Only
1 (5.0%) of the 20 PZA-monoresistant isolates had spoligotyping patterns characteristic  Therefore,
the CAS superfamily spoligotypes showed no important correlation with MDR-TB as 

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Epidemiology of Mycobacterium bovis infection in wild boar (Sus scrofa) from Portugal

[HTML] from jwildlifedis.org

N Santos, M Correia-Neves… – Journal of wildlife …, 2009 – Wildlife Dis Assoc

 signatures were compared with those available in SpolDB4.0, the international spoligotyping
database (Brudey et al  The spoligotypes of 14 M. bovis isolates obtained from study areas B and
 originated from study area B. Isolates from the study area C were not spoligotyped

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Avaliação da técnica de spoligotyping aplicada diretamente em amostras clínicas de pacientes com suspeita de tuberculose e caracterização epidemiológica

[PDF] from ufrgs.br

PI Cafrune – 2009 – lume.ufrgs.br

 Spoligotyping technique presented good results when applied directly to clinical samples, once
89% of samples presented agreeing results. Among 135 patients isolates genotyped, it was found
44 differnt spoligotypes, 112 (83%) were shared types and 23(17%) were unique. 

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Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda

BB Asiimwe, J Asiimwe, G Kallenius… – Veterinary …, 2009 – veterinaryrecord.bmj.com

 The isolates were spoligotyped using the DNA extracted at the identification stage.  The
spoligotypes were analysed by BioNumerics software, version 5.0 (Applied Maths) as character 
were compared with those in SpolDB4.0, the international spoligotyping database (Brudey 

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High resolution typing system for pathogenic Mycobacterium tuberculosum

PS Keim, RS Spurgiesz… – US Patent 7,592,135, 2009 – Google Patents

 Spoligotyping is currently the most widely used PCR- based method of M. tuberculosum
genotyping due to the 65 relative technical ease of  In a database of 3,319 spoligotypes from
isolates collected in geographically distinct areas, seven spoligotype patterns were found for 

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Frequency of Mycobacterium bovis as an etiologic agent in extrapulmonary tuberculosis in HIV-positive and-negative Mexican patients

R Cicero, H Olivera, A Hernández-Solis… – European journal of …, 2009 – Springer

 a commercially available kit in accordance with the manufacturer’s recommendations (Isogen
Bioscience BV  and consistent identification of M. bovis was obtained by spoligotyping, showing
 cattle, where even isolates from the same herd show different spoligotypes [26] and 

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[HTML] In-depth molecular characterization of Mycobacterium tuberculosis from New Delhi–predominance of drug resistant isolates of the ‘modern'(TbD1−) type

[HTML] from plos.org

R Stavrum, VP Myneedu, VK Arora, N Ahmed… – PloS one, 2009 – dx.plos.org

 C membranes with the 43 synthetic oligonucleotides covalently bound to the membranes (Isogen
Bioscience BV  (2004) Predominant tuberculosis spoligotypes, Delhi, India.  repetitive-
unit-variable-number tandem-repeat typing system combined with spoligotyping for population 

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Predominance of Mycobacterium tuberculosis EAI and Beijing lineages in Yangon, Myanmar

[HTML] from nih.gov

S Phyu, R Stavrum, T Lwin… – Journal of clinical …, 2009 – Am Soc Microbiol

 (35). Spoligotyping was performed with commercially available activated Biodyne C membranes
that had 43 synthetic oligonucleotides covalently bound to the membranes (Isogen Bioscience
BV  Prevalence of spoligotypes in Yangon.The spoligotyping results obtained 

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[HTML] Tokyo-172 BCG vaccination complications, Taiwan

[HTML] from nih.gov

R Jou, WL Huang… – Emerging Infectious Diseases, 2009 – ncbi.nlm.nih.gov

 BCG, DNA samples were initially screened using a GenoType kit (Hain Lifescience GmbH, Nehren,
Germany), multiplex PCR (7), and pncA sequencing (8). In addition, spoligotyping was performed
with a commercial kit (Isogen Bioscience BV, Maarssen, the Netherlands). 

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Immunologic analysis of HIV-uninfected taiwanese children with BCG-induced disease

WI Lee, FC Liang, JL Huang, TH Jaing… – Journal of clinical …, 2009 – Springer

 also used to identify and differentiate M. tuberculosis complex isolates in this study [38], using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). The resolved spoligotypes were M. tuberculosis complex strain specific. The spoligotyping profile of 

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A rapid and simple method for identifying Mycobacterium tuberculosis W-Beijing strains based on detection of a unique mutation in Rv0927c by PCR-SSCP

X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier

 Spoligotyping was performed as previously described [16  in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for 

Cited by 5 – Related articles – All 7 versions – Import into BibTeX

[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

[PDF] from lrsitbrd.nic.in

TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in

Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 

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Beijing genotype of Mycobacterium tuberculosis is significantly associated with high-level fluoroquinolone resistance in Vietnam

[HTML] from nih.gov

DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol

 with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV  were assigned to the Euro-American clade on the basis of spoligotyping patterns 
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive 

Cited by 23 – Related articles – All 9 versions – Import into BibTeX

Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol

 In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in 
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9  performed using a kit according to the manufacturer’s instructions (Isogen/Omicium 

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[HTML] Supervised learning for the automated transcription of spacer classification from spoligotype films

[HTML] from biomedcentral.com

D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com

 a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen  Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a  AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of 

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Potential challenges to the Stop TB Plan for humans in China; cattle maintain M. bovis and M. tuberculosis

[PDF] from yimg.com

Y Chen, Y Chao, Q Deng, T Liu, J Xiang, J Chen… – Tuberculosis, 2009 – Elsevier

 Spoligotyping was performed to define the genotype of Beijing family strains with a
commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s instruction. The spoligotype that only contained 

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[PDF] Tuberculosis Control in Oman

[PDF] from ki.se

A Al-Maniri – 2009 – diss.kib.ki.se

 Spoligotyping of 312 isolates of Mycobacterium tuberculosis from TB patients
diagnosed during 2005-2007 was carried out in study IV.  25 6.5 Study IV: Biodiversity
and clustering of M.tuberculosis isolates using spoligotyping ….. 

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[PDF] Molekularbiologischer Nachweis des Mycobacterium tuberculosis-Komplexes in altägyptischen Mumien unter besonderer Berücksichtigung neuer …

[PDF] from uni-muenchen.de

N Motamedi – 2009 – edoc.ub.uni-muenchen.de

 modernen und der ursprünglichen Form von M. tuberculosis. Van Embden et al. 68
untersuchten M. canetti mit einem erweiterten Spoligotyping (siehe unten), das mehr als
die 43 Spacer der etablierten Technik aufsucht. Hier fielen Spacer auf, 

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[HTML] Tuberculosis disparity between US-born blacks and whites, Houston, Texas, USA

[HTML] from nih.gov

JA Serpa, LD Teeter, JM Musser… – Emerging infectious …, 2009 – ncbi.nlm.nih.gov

 isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic  the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV 

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Molecular characterization of Mycobacterium Bovis isolates from selected slaughter houses in Kampala

[PDF] from mak.ac.ug

J Asiimwe – 2009 – dspace.mak.ac.ug

 Spoligotyping revealed that three of the M. bovis isolates had spoligopatterns that had previously
been reported in Uganda while the seven  Although a few clusters were identified on analysis
of the spoligotypes and RFLP patterns, no geographical sub-structuring was observed 

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Predictors of poor treatment outcome in multi-and extensively drug-resistant pulmonary TB

[HTML] from ersj.org.uk

K Kliiman… – European Respiratory Journal, 2009 – ersj.org.uk

 The strains were spoligotyped as described by Kamerbeek et al. 17 using commercially available
membranes (Isogen, Maarssen, The Netherlands) 17.  Mycobacterium tuberculosis complex genetic
diversity: mining the fourth international spoligotyping database (SpolDB4) for 

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[HTML] Tuberculosis Disparity between US-born Blacks and Whites, Houston, Texas, USA

[HTML] from cdc.gov

F Figures, I Capitalization, AS Guides… – Tuberculosis, 2009 – wwwnc.cdc.gov

 isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic  the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV 

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Molecular epidemiology of Mycobacterium tuberculosis in the United Arab Emirates (UAE)

[DOC] from qmul.ac.uk

A Saaidi… – 2009 – qmro.qmul.ac.uk

 HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 

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[PDF] EPIDEMIOLOGÍA MOLECULAR DE LA

[PDF] from ugr.es

TEN ALMERÍA – hera.ugr.es

Page 1. FACULTAD DE MEDICINA UNIVERSIDAD DE GRANADA DEPARTAMENTO
DE MEDICINA TÍTULO TESIS DOCTORAL: EPIDEMIOLOGÍA MOLECULAR DE LA
TUBERCULOSIS EN ALMERÍA Page 2. Editor: Editorial 

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2008

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Spoligotypes of< i> Mycobacterium tuberculosis</i> isolates of a high tuberculosis burden aboriginal township in Taiwan

PC Chuang, H Liu, C Sola, YMA Chen… – Infection, Genetics and …, 2008 – Elsevier

 A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as  All 138 isolates were successfully genotyped by spoligotyping, but only 122 were
of  A total of 27 spoligotypes were identified; 124 isolates (89.9%, 124/138) were 

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Genetic diversity of isoniazid-resistant Mycobacterium tuberculosis isolates collected in Poland and assessed by spoligotyping

[HTML] from nih.gov

E Augustynowicz-Kopeć, T Jagielski… – Journal of clinical …, 2008 – Am Soc Microbiol

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands), according to the manufacturer’s instructions and as  All spoligotypes obtained
were compared to those in the world spoligotyping database (SpolDB4) at 

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[HTML] Mycobacterium tuberculosis spoligotypes and drug susceptibility pattern of isolates from tuberculosis patients in peri-urban Kampala, Uganda

[HTML] from biomedcentral.com

B Asiimwe, S Ghebremichael… – BMC infectious …, 2008 – biomedcentral.com

 The obtained signatures were compared in binary format with the international spoligotyping
database of the Pasteur Institute of  Spoligotypes. To determine the strain lineages present in the
sample, the 344 isolates were spoligotyped and binary outcomes compared with those 

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Molecular typing of Mycobacterium tuberculosis isolates from a rural area of Kanpur by spoligotypingand mycobacterial interspersed repetitive units (MIRUs) typing

P Sharma, DS Chauhan, P Upadhyay… – Infection, Genetics and …, 2008 – Elsevier

 2.3. SpoligotypingSpoligotyping to detect 43 spacers was done with the help of commercially
available kit (Isogen Biosciences, BV, Maarsen, The Netherlands).  3.1. Spoligotyping. A total of
46 distinct spoligotypes were obtained from this set of 97 M. tuberculosis isolates. 

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Role of spoligotyping and IS6110-RFLP in assessing genetic diversity of< i> Mycobacterium tuberculosis</i> in India

JP Mathuria, P Sharma, P Prakash… – Infection, Genetics and …, 2008 – Elsevier

 spacers by using the standard method (Kamerbeek et al., 1997) with the help of commercially
available kit (Isogen Biosciences, BV  Spoligotyping Presence of 19 orphan isolates indicates
the need of conducting multicentric studies to know all the prevalent spoligotypes in Indian 

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[PDF] Investigation of Cross-Contamination in a Mycobacterium tuberculosis Laboratory Using Epidemiological data and Spoligotyping

[PDF] from pjms.com.pk

F Al-Otaibi… – pjms.com.pk

 Spoligotyping was performed with a commer- cially available kit (Isogen Bioscience).6,7 RESULTS 
Culturing of phosphate buffer in MGIT tube was positive for MTB after seven-day inoculation. Ten
different spoligotypes were identified among the 47 MTB isolates. 

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Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation

[PDF] from cnrs.fr

B Romero, A Aranaz, Á Sandoval, J Álvarez… – Veterinary …, 2008 – Elsevier

 detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV  The discriminatory capacity of spoligotyping was h = 0.38  The spoligotypes SB0950,
SB1230 and SB1232 were shared between domestic and wildlife animals (red 

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Spoligotyping of Mycobacterium tuberculosis in anthracotic bronchitis

K Najafizadeh, F Ghorbani, P Farnia… – … of Tuberculosis and …, 2008 – ingentaconnect.com

 use at −20°C. Spoligotyping Spoligotyping was performed, as previously described
by Kamerbeek et al.,10 with a commercially available kit (Isogen Bioscience BV,
Maarssen, The Netherlands). Analysis Information about the 

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Molecular analysis of drug‐resistant Mycobacterium tuberculosis isolates collected in central Poland

E Augustynowicz‐Kopeć, T Jagielski… – Clinical …, 2008 – Wiley Online Library

 DNA was prepared by the cetyl-trimethyl-ammonium bromide method [4]. Spoligotyping was
performed using a commercially available kit (Isogen Bioscience BV  was also performed as
described previously [2]. Isolates with indistinguishable spoligotypes and IS6110 

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[HTML] Human tuberculosis caused by Mycobacterium bovis, Taiwan

[HTML] from nih.gov

R Jou, WL Huang… – Emerging infectious diseases, 2008 – ncbi.nlm.nih.gov

 Currently, 119 M. bovis spoligotypes are contained in the fourth international spoligotyping
database (SpolDB 4) and  strains, presence or absence of region of difference 1 was analyzed
(5). Spoligotyping was performed with a commercial kit (Isogen Bioscience BV 

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A proposal for applying molecular markers as an aid to identifying potential cases of imported tuberculosis in immigrants

M Alonso, S Borrell, MM Lirola, E Bouza… – Tuberculosis, 2008 – Elsevier

 Spoligotyping was performed using primers of the spoligotyping kit (Isogen, Netherlands) and
following the manufacturer’s instructions. The spoligotypes were analyzed in the MIRU-VNTRplus
application (www.miru-vntrplus.org) 19 in order to identify the genetic lineages 

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Characterization of extensively drug-resistant Mycobacterium tuberculosis clinical isolates in China

[HTML] from nih.gov

Z Sun, Y Chao, X Zhang, J Zhang… – Journal of clinical …, 2008 – Am Soc Microbiol

 repeat (MIRU-VNTR) analysis. Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s protocols. PCR-based MIRU-VNTR analysis 

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[HTML] Incidence of tuberculosis and the predictive value of ELISPOT and Mantoux tests in Gambian case contacts

[HTML] from plos.org

PC Hill, DJ Jackson-Sillah, A Fox, RH Brookes… – PLoS One, 2008 – dx.plos.org

 For molecular subtyping of index case isolates, we extracted mycobacterial DNA using CTAB
and chloroform[18]. We performed spoligotyping using membranes from Isogen Biosciences
(http:/www.isogen-lifescience.com), as previously described[19]. 

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[PDF] : Transmission from South American Sea Lion () to Bactrian Camel () and Malayan Tapirs ()

[PDF] from cnrs.fr

I Moser, WM Prodinger, H Hotzel… – Veterinary …, 2008 – peer.ccsd.cnrs.fr

 by 30 cycles of 96°C for 15 sec, 67°C for 60 sec, 72°C for 60 sec, and a final extension at 18
72°C 300 sec. 19 SpoligotypingSpoligotyping (9; Isogen Bioscience, The Netherlands) was
performed using 20 DNA extracted from cultures. 21 MIRU typing. 

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Use of PCR-based Mycobacterium tuberculosis genotyping to prioritize tuberculosis outbreak control activities

[HTML] from nih.gov

M Ashworth, KL Horan, R Freeman… – Journal of clinical …, 2008 – Am Soc Microbiol

 Only one isolate per case was included in the analysis. Genotyping.Spoligotyping
was performed according to previously published methods by using a spoligotyping
kit (Isogen, IJsselstein, The Netherlands) (12). MIRU analysis 

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Mycobacteria causing human cervical lymphadenitis in pastoral communities in the Karamoja region of Uganda

[HTML] from nih.gov

J Oloya, J Opuda-Asibo, R Kazwala… – Epidemiology and …, 2008 – Cambridge Univ Press

 MTC were submitted for further differentiation by the spoligotyping kit ac- cording to the
manufacturer’s instructions (Isogen, Life Science  One of the spoligotypes (Hu-4 and Hu-5 in Fig.
1) is labelled LAM 3/S in the spoligotyping database SpolDB4, and has been isolated in 118 

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Genotypic diversity of extensively drug-resistant tuberculosis (XDR-TB) in South Africa

CK Mlambo, RM Warren, X Poswa… – … of tuberculosis and …, 2008 – ingentaconnect.com

 Spoligotyping was performed ac- cording to the internationally standardised protocol11 using
commercially  of the genotyping data, serial isolates from 13 patients were randomly sampled
and spoligotyped was used to assign geno- type families to the spoligotypes obtained.12 

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[HTML] Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei

[HTML] from biomedcentral.com

HY Dou, FC Tseng, CW Lin, JR Chang… – BMC infectious …, 2008 – biomedcentral.com

 Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not 
Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV  The resulting spoligotypes were documented under a binary code representing either a 

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[HTML] Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan

[HTML] from biomedcentral.com

M Tanveer, Z Hasan, A Siddiqui, A Ali… – BMC Infectious …, 2008 – biomedcentral.com

 Spoligotyping of M. tuberculosis isolates. All M. tuberculosis strains were spoligotyped and their
data analyzed using UPGMA calculation in the Bionumerics software.  one (78%) isolates grouped
into 59 different “shared types” while 205 (22%) isolates had ‘orphan’ spoligotypes

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< i> Mycobacterium pinnipedii</i>: Transmission from South American sea lion (< i> Otaria byronia</i>) to Bactrian camel (< i> Camelus bactrianus bactrianus</i>) and …

[PDF] from tcd.ie

I Moser, WM Prodinger, H Hotzel, R Greenwald… – Veterinary …, 2008 – Elsevier

 2.5. SpoligotypingSpoligotyping (Kamerbeek et al., 1997; Isogen Bioscience, The Netherlands)
was performed using DNA extracted from cultures. 2.6. MIRU typing. MIRU typing was
performed targeting 12 chromosomal loci as given by Mazars et al. 

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Mycobacterium tuberculosis population structures differ significantly on two Indonesian Islands

[HTML] from nih.gov

I Parwati, R Van Crevel, M Sudiro… – Journal of clinical …, 2008 – Am Soc Microbiol

 Therefore, we have spoligotyped M. tuberculosis isolates from a large cohort of patients 
Mycobacterium tuberculosis complex genetic diversity: mining the fourth international
spoligotyping database (SpolDB4) for  Global distribution of Mycobacterium tuberculosis 

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Development of multiplex assay for rapid characterization of Mycobacterium tuberculosis

[HTML] from nih.gov

IL Bergval, R Vijzelaar, ER Dalla Costa… – Journal of clinical …, 2008 – Am Soc Microbiol

 Strains 1 to 29 were selected for their specific spoligotypes and were used to determine the
correlation between MLPA, the spoligotype, and the sequence of ogt codon 15 (a marker for the
 Spoligotyping.Mycobacterium tuberculosis strains were spoligotyped by using a 

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Mycobacterium bovis in wildlife in France

[HTML] from jwildlifedis.org

G Zanella, B Durand, J Hars… – Journal of wildlife …, 2008 – Wildlife Dis Assoc

 Biotin-labeled PCR products were detected on a spoligotyping membrane (Isogen Bioscience BV,
Maarssen, The Netherlands). Purified sterile water and M. bovis and M. tuberculosis controls
included in the spoligomembrane kit were included in each batch of tests. 

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Distribution of rpoB mutations among multidrug‐resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation …

T Prammananan, W Cheunoy… – Clinical …, 2008 – Wiley Online Library

 In contrast, of 112 rifampicin-susceptible isolates that were spoligotyped, only 51 (45.5%) were
revealed to be  a One additional MDRTB isolate was not available for spoligotyping Association
of specific mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug 

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Molecular diversity of< i> Mycobacterium tuberculosis</i> isolates from patients with pulmonary tuberculosis in Sri Lanka

US Rajapaksa, TC Victor, AJ Perera… – Transactions of the …, 2008 – Elsevier

 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV, Maarssen,
The  Spoligotyping of 98 M. tuberculosis isolates revealed a total of 43 patterns, with six  The finding
of identical spoligotypes in geographically widely separated countries may be 

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[PDF] Mutations in rpoB gene and genotypes of rifampin resistant Mycobacterium tuberculosis isolates in Iran

[PDF] from nritld.ac.ir

F Doustdar, AD Khosravi, P Farnia, AR Bahrmand… – Tanaffos, 2008 – nritld.ac.ir

 tuberculosis isolates are defined on the basis of shared spoligotypes or closely related IS6110 
Spoligotyping was performed with a commercially available kit to detect 43 known spacers  DR
locus according to the instructions supplied by the manufacturer (Isogen Bioscience BV 

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Molecular epidemiology of Mycobacterium bovis: usefulness in international trade

[PDF] from usda.gov

F Milian-Suazo, B Harris, CA Díaz… – Preventive veterinary …, 2008 – Elsevier

 Spoligotyping was performed following the protocol described by Kamerbeek et al  Eighty-four
M. bovis strains from Mexico and the US were spoligotyped under the same protocol in two  When
comparing spoligotypes from the two laboratories, 50 (59%) out of the 84 strains were 

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Attempts to revive Mycobacterium tuberculosis from 300‐year‐old human mummies

E Lemma, O Zimhony, CL Greenblatt… – FEMS microbiology …, 2008 – Wiley Online Library

 Spoligotyping was performed at day 250 following the instructions of the manufacturer
of the ISOGEN Life Science Spoligotyping kit. Animal inoculations. Two sets of six
female guinea pigs weighing an average of 250 g were used. 

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Mycobacterium tuberculosis Uganda genotype is the predominant cause of TB in Kampala, Uganda

[PDF] from mak.ac.ug

BB Asiimwe, T Koivula, G Kallenius… – … of Tuberculosis and …, 2008 – ingentaconnect.com

 Data analysis Spoligotypes were analysed using BioNumerics soft- ware (version 4.6, Applied
Maths, Kortrijk, Belgium) as a character type.  Spoligotyping To determine the predominant strain
lineages, the 344 isolates were spoligotyped and binary outcomes compared with 

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Pyrazinamide resistance among South African multidrug-resistant Mycobacterium tuberculosis isolates

[HTML] from nih.gov

M Mphahlele, H Syre, H Valvatne… – Journal of clinical …, 2008 – Am Soc Microbiol

 (i) Spoligotyping.Spoligotyping was performed to further  The resulting amplification products were
then hybridized to a commercially available membrane (Isogen Bioscience BV, Bilthoven, The
Netherlands), with covalently linked parallel rows of 43 synthetic oligonucleotides 

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Genotyping North American animal Mycobacterium bovis isolates using multilocus variable number tandem repeat analysis

[HTML] from sagepub.com

LR Martinez, B Harris, WC Black… – Journal of Veterinary …, 2008 – vdi.sagepub.com

 The 6 isolates that comprised MLVA type N were divided into 3 spoligotypes: 1 isolate into SP2 
A larger database of MLVA typed isolates, in combination with spoligotyping and conventional
epidemiological investigations, can provide a  n. Spacer sequences, Isogen Life Science 

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[CITATION] First Insight Into Mycobacterium tuberculosis Biodiversity in Sri Lanka: Evidence for Predominance of Beijing Genotype and Ancestral Lineages Among …

US Rajapaksa, AJ Perera, TC Victor… – International Journal of …, 2008 – Elsevier

Import into BibTeX

[BOOK] Molecular characterization of Mycobacterium tuberculosis complex from Kampala, Uganda

[PDF] from mak.ac.ug

B Asiimwe – 2008 – dspace.mak.ac.ug

 and colleagues (69), which is commercially available as a kit (Isogen Bioscience BV  the important
lineages and sub-lineages in SpolDB4.0, the international spoligotyping database iii  To investigate
the association between the predominant spoligotypes, HIV sero- status and anti 

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[PDF] A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon

[PDF] from sote.hu

L Judit – phd.sote.hu

Page 1. A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon
Doktori értekezés Lukács Judit Semmelweis Egyetem Klinikai Orvostudományok
Doktori Iskola Témavezetı: Dr. Somoskövi Ákos, MTA doktora 

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IS6110 in oriC affects the morphology and growth of Mycobacterium tuberculosis and attenuates virulence in mice

Y Casart, L Turcios, I Florez, R Jaspe, E Guerrero… – Tuberculosis, 2008 – Elsevier

 DR locus was carried out as described earlier, 15 with membranes supplied by Isogen Bioscience
BV  to mention that all the clones isolated from 1C8 or 1D3 displayed identical spoligotyping and
MIRU  A1 insertion 12 (Figure 1B), (ii) The IS6110-RFLPs and spoligotypes did not 

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Intérêt du typage moléculaire pour l’épidémiologie de la tuberculose en Limousin (1998–2006)

[HTML] from em-consulte.com

H Bezanahary, MC Baclet, C Sola… – Médecine et Maladies …, 2008 – Elsevier

 Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping L’hybridation (1H à 60
°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV  sous forme d’un
code octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut 

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[PDF] TABLA 8.1 _ GEL DE CORRIMIENTO

[PDF] from ula.ve

TNN – Identificación y diagnóstico de Actinomicetales … – saber.ula.ve

 caontb. rivm. nl/Manuals/principl. html. Por su parte, Isogen Biosciences (http://www. isogen.
nl) ofrece membranas con ADN inmobilizado de espaciadores, para utilizarse en pruebas
de spoligotyping específicas para cepas de M. bovis/M. tuberculosis. 

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Diversidade genética de estirpes de mycobacterium tuberculosis em pacientes com tuberculose nos distritos de Buzi e Manhiça, em Moçambique

[PDF] from utl.pt

CLS Mucavele – 2008 – repository.utl.pt

 number of spoligotypes were identical to profiles found in the region. The data reported showed
that migration and HIV might be factors of dissemination of TB in Humans in Mozambique. Factors
that can compromise the performance of Spoligotyping technique and other 

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[BOOK] Molecular Characterisation of Antibiotic Resistance in Mycobacterium Tuberculosis

[PDF] from ki.se

P Juréen – 2008 – diss.kib.ki.se

 personnel (91). In order to overcome this, spoligotyping was evolved. This  repeat) (91).
MIRU-VNTR analyses copy-numbers of repeats, however, in contrast to spoligotyping,
these have several sites scattered around the genome. Since 

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2007

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Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis

[PDF] from mvlab-fudan.cn

J Chen, AG Tsolaki, X Shen, X Jiang, J Mei… – Tuberculosis, 2007 – Elsevier

 Spoligotyping was performed by using an Isogen Bioscience spoligotyping kit (Isogen Life Science 
Figure 3. Beijing/W Spoligotypes observed in 182 isolates with deletion RD105. Comparison
of the multiplex PCR with spoligotyping revealed 100% sensitivity and specificity for 

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Contribution of spoligotyping to the characterization of the population structure of Mycobacterium tuberculosis isolates in Portugal

[TXT] from min-saude.pt

S David, DR Ribeiro, A Antunes, C Portugal… – Infection, Genetics and …, 2007 – Elsevier

 Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV, Maarsen, Netherlands). The resulting spoligotypes were documented under a binary code
representing either a positive or negative hybridization result (n and o) and analyzed 

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Spoligotyping of< i> Mycobacterium tuberculosis</i> DNA from Archival Ziehl–Neelsen-stained sputum smears

N Suresh, J Arora, H Pant, T Rana… – Journal of microbiological …, 2007 – Elsevier

 available kit, according to the instructions supplied by the manufacturer (Isogen Bioscience, BV 
a positive signal in spoligotyping, thus demonstrating the efficiency of spoligotyping in a  analysis
of DNA extracted from stored ZN slides identified tuberculosis spoligotypes in 41 

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Improvement of spoligotyping with additional spacer sequences for characterization of Mycobacterium bovis and M. caprae isolates from Spain

MT Javed, A Aranaz, L De Juan, J Bezos… – Tuberculosis, 2007 – Elsevier

 using two membranes: a commercial membrane containing the 43 spacers described earlier
23 (Isogen Bioscience BV  Number of spoligotyping patterns of M. bovis and M. caprae.
Thirty-one different spoligotypes were identified among the 308 M. bovis (26 patterns) and 88 

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Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

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H Han, F Wang, Y Xiao, Y Ren… – Journal of medical …, 2007 – Soc General Microbiol

 To test the reproducibility of MIRU typing, we blindly typed 20 isolates in two independent
experiments. SpoligotypingSpoligotyping was performed with a commercial spoligotyping kit
(Isogen Bioscience), according to the instructions supplied by the manufacturer. 

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[PDF] … tuberculosis Beijingg family isolates from Hong Kong and comparisonn with 1S6110 restriction fragment lengthh polymorphism typing and spoligotyping

[PDF] from uva.nl

K Kremer, BKY Au, PCW Yip, R Skuce, P Supply… – dare.uva.nl

 with a commercial membranee containing the standard set of 43 spacer oligonucleotides (Isogen
Bio- sciencee BV  The usee of spacers 56, 57, 66, 67, and 68 in spoligotyping increased the  patterns
among the Beijing lineage strains in this study from three to five spoligotypes.. 

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Identification of Beijing lineage Mycobacterium tuberculosis with combined mycobacterial interspersed repetitive unit loci 26, 31, and ETR-A

[HTML] from nih.gov

PJ Chin, CC Chiu… – Journal of clinical microbiology, 2007 – Am Soc Microbiol

 These isolates were characterized by IS6110 RFLP following standard protocols (12) and
spoligotyping with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands)
(6). The characteristic  All other spoligotypes are defined as non-Beijing genotypes. 

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Znaczenie metody spoligotyping w epidemiologicznych dochodzeniach gruźlicy

[PDF] from viamedica.pl

E Augustynowicz-Kopeć… – Pneumonologia i …, 2007 – pneumonologia.viamedica.pl

 Autorzy dziękują Pani J. Scheenhart (Isogen Bioscience BV) za konsultację warunków repro 
IS6110 restriction fragment length polymor- phism patterns and spoligotypes determined by 
Spoligotyping of Myco- bacterium tuberculosis isolates from patients with pulmo- nary 

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[HTML] Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories

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C Rodrigues, D Almeida, S Shenai, N Goyal… – Indian Journal of …, 2007 – Medknow

 Spoligotyping Spoligotyping was performed with a commercially available kit (Isogen Bioscience). 
Remaining two isolates grown from smear positive specimens had different spoligotypes hence
were  Since spoligotyping is known to be less refractory for differentiation of M 

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Special Article-Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories

[PDF] from bioline.org.br

C Rodrigues, D Almeida, S Shenai… – Indian Journal of …, 2007 – bioline.org.br

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience).  Remaining
two isolates grown from smear positive specimens had different spoligotypes hence were  Since
spoligotyping is known to be less refractory for differentiation of M. tuberculosis, we 

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[HTML] Characterisation of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda

[HTML] from biomedcentral.com

J Oloya, R Kazwala, A Lund… – BMC …, 2007 – biomedcentral.com

 in mid-central and mid-western areas of Uganda and detected new and specific spoligotypes
not reported  Cultures belonging to the M. tuberculosis complex were submitted for further
differentiation by Spoligotyping [20] as described by the producer (Isogen, Life science 

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Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey

O Kisa, A Albay, O Baylan, E Tozkoparan… – Research in …, 2007 – Elsevier

 Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according to the  2.6. Comparison of spoligotypes with the international spoligotype
database. Spoligotyping results were entered in a binary format and octal code on Excel 

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Evolution of the extensively drug-resistant F15/LAM4/KZN strain of Mycobacterium tuberculosis in KwaZulu-Natal, South Africa

[HTML] from oxfordjournals.org

M Pillay… – Clinical infectious diseases, 2007 – cid.oxfordjournals.org

 SpoligotypingSpoligotyping was performed using a kit from Isogen-Life Science
in accordance with the manufacturer’s instructions. The presence or absence of 43
spacers in the direct-repeat region of isolates of M. tuberculosis 

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[HTML] Longitudinal assessment of an ELISPOT test for Mycobacterium tuberculosis infection

[HTML] from plos.org

PC Hill, RH Brookes, A Fox, D Jackson-Sillah… – PLoS medicine, 2007 – dx.plos.org

 We performed spoligotyping using membranes from Isogen Biosciences (http://www.isogen
lifescience.com), as previously described [18] and analysed the results with software that we
designed using Matlab software (MathWorks, http://www.mathworks.com). 

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[HTML] M. tuberculosis genotypic diversity and drug susceptibility pattern in HIV-infected and non-HIV-infected patients in northern Tanzania

[HTML] from biomedcentral.com

GS Kibiki, B Mulder, WMV Dolmans… – BMC …, 2007 – biomedcentral.com

 with samples which were INH-resistant, contained phenotypic rifampicin-resistant strains, or had
selected spoligotypes; phenotypic rifampicin  Spoligotyping 1 U/μl). Amplification and hybridization
were performed according to the manufacturer’s manual (Isogen Bioscience BV 

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Outbreak of Mycobacterium bovis infection in a wild animal park

SM Schmidbauer, P Wohlsein, G Kirpal… – Veterinary …, 2007 – veterinaryrecord.bmj.com

 and subtype of the MTC agent, 17 isolates from various organs were spoligotyped (Kamerbeek
and  FIG 2: Spoligotyping pattern of 17 mycobacterial isolates from various organs from seven 
in wild animals in Germany and in these cases the spoligotypes normally correspond to 

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Molecular investigation of recurrent tuberculosis in patients from Rwanda

AN Umubyeyi, IC Shamputa… – … of Tuberculosis and …, 2007 – ingentaconnect.com

 DNA fingerprinting techniques Spoligotyping was performed using a commercial kit (Isogen
Bioscience BV  4 of the 12 previously cured MDR-TB patients showed different spoligotypes,
suggesting re  of their recurrence (Figures 1 and 2). In contrast, the spoligotyping pat- terns of 

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[HTML] Using ELISPOT to expose false positive skin test conversion in tuberculosis contacts

[HTML] from plos.org

PC Hill, DJ Jeffries, RH Brookes, A Fox… – PLoS One, 2007 – dx.plos.org

 spectrophotometry. We performed Spoligotyping using membranes (Isogen
Biosciences), as previously described,[14] scanned the results and analysed them
with software designed in Matlab. Data management and analysis. 

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[PDF] Usefulness of molecular techniques to identify ongoing tuberculosis transmission in Saudi Arabia

[PDF] from smj.org.sa

SA Al-Hajoj, VK Mohammed… – Saudi medical journal, 2007 – smj.org.sa

 The labeled PCR product was used as a probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV, Maarssen, The
Netherlands). The 2 isolates were identical by both RFLP and spoligotyping methods Figure 

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Molecular clues of a microepidemy among homeless tuberculosis patients in Budapest due to a new and local Mycobacterium tuberculosis clade

C Ködmön, S Niemann, MC Gutierrez… – Infection, Genetics and …, 2007 – Elsevier

 Spoligotyping was performed with a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands) according to the instructions of the manufacturer
(Kremer et al., 1999). Briefly, genomic mycobacterial DNA 

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Discovery of a novel Mycobacterium tuberculosis lineage that is a major cause of tuberculosis in Rio de Janeiro, Brazil

[HTML] from nih.gov

LCO Lazzarini, RC Huard, NL Boechat… – Journal of clinical …, 2007 – Am Soc Microbiol

 Spoligotyping.All M. tuberculosis strains were spoligotyped using a commercial kit (Isogen
Bioscience BV  as an octal code (9). The person who performed the spoligotyping was blinded
to  information on the shared-type distributions of M. tuberculosis spoligotypes worldwide (N 

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Genotype and phenotype relationships and transmission analysis of drug-resistant tuberculosis in Singapore

YJ Sun, ASG Lee, SY Wong… – … of Tuberculosis and …, 2007 – ingentaconnect.com

 Spoligotyping Spoligotyping was performed using a commercial kit obtained from Isogen
Bioscience BV  as previously described.21,22 IS6110 RFLP patterns and/or MIRU-VNTR patterns
were used to determine genotypes for a few isolates with indeterminate spoligotypes

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Evidence of ‘amplifier effect’in pulmonary multidrug-resistant tuberculosis: report of three cases

A Umubyeyi, IC Shamputa, L Rigouts… – International Journal of …, 2007 – Elsevier

 SpoligotypingSpoligotyping was performed with a commercial kit (Isogen Bioscience
BV, Maarssen, The Netherlands) by the previously described method. 10 Briefly, 4
μl of the heat-killed bacterial suspension from each sample 

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[PDF] STUDY OF ISONIAZID-RESISTANT TUBERCULOSIS: MYCOBACTERIAL GENOTYPIC CHARACTERIZATION AND CLINICAL FEATURES IN HUMAN

[PDF] from usc.edu

P Escalante – 2007 – digitallibrary.usc.edu

 BioImage Whole Band Analyzer™, version 3.2 software (BioImage, Ann Arbor, MI). Spoligotyping
was performed using the Isogen™ kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all
isolates according to the manufacturer instructions. In addition, all 

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[PDF] GENOTIPAGEM UTILIZANDO A SEQÜÊNCIA DE INSERÇÃO IS6110 E “SPOLIGOTYPING” DE Mycobacterium tuberculosis ISOLADOS DE PACIENTES …

[PDF] from sp.gov.br

AC PANUNTO – crt.saude.sp.gov.br

 35 Tabela 3- Perfis agrupados de IS6110-RFLP presentes no estudo….. 50
Tabela 4- Perfis agrupados de “Spoligotyping” presentes no estudo….. 53
Tabela 5- “Spoligotypes” mais freqüentes no estudo….. 54 

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[BOOK] Study of isoniazid-resistant tuberculosis: Mycobacterial genotypic characterization and clinical features in human subjects

P Escalante – 2007 – books.google.com

 software (BioImage, Ann Arbor, MI). Spoligotyping was performed using the Isogen
kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all isolates according to
the manufacturer instructions. In addition, all isolates also 

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First insight into the population structure of Mycobacterium tuberculosis in Saudi Arabia

[HTML] from nih.gov

SAM Al-Hajoj, T Zozio, F Al-Rabiah… – Journal of clinical …, 2007 – Am Soc Microbiol

 to inter-DR spacer oligonucleotides covalently bound to a membrane (Isogen Life Science  All
spoligotypes were converted into octal format within Excel spreadsheets (8) and  The individual
spoligotyping patterns were compared with an updated SpolDB4 database named 

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Transmission of< i> Mycobacterium tuberculosis</i> in a Family Proved by Genotyping

CY Yang, R Jou, PC Chuang, JT Chang… – Journal of the Formosan …, 2007 – Elsevier

 Spoligotyping Spoligotyping is a polymerase chain reaction (PCR)-based analysis that uses
a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands).  The re-
solved spoligotypes were M. tuberculosis complex strain-specific. 

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[PDF] Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey

[PDF] from microbiologica.net

C Bicmen, N Esen, EA Graviss… – … -Quarterly Journal of …, 2007 – microbiologica.net

 were analyzed with the BioImage (Ann Arbor, Mich.,USA) Whole Band Analyzer, version 3.4.2.
Spoligotyping was per- formed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) in accordance with the manufacturer’s recom- mendations. 

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Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis

[HTML] from nih.gov

P Farnia, MR Masjedi, B Nasiri… – Epidemiology and …, 2007 – Cambridge Univ Press

 [12] with a commercially avail- able kit (Isogen Bioscience BV  Stability of M. tuberculosis IS6110
restriction fragment length polymorphism patterns and spoligotypes determined by  expanding
clones of M. tuberculosis and their global distribution assessed by spoligotyping in an 

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Identification of unique genetic markers in Rv0927c among< i> Mycobacterium tuberculosis</i> W-Beijing strains

X Jiang, Y Zhang, W Zhang, F Gao, J Jin… – Microbes and …, 2007 – Elsevier

 SDS) at 60 °C and then incubated in 1:4000 diluted streptavidin–peroxidase conjugate (Isogen
Bioscience) for  This finding, which showed a spoligotyping pattern with hybridization only to the
spacer 35 to 43  1. Spoligotypes of the 56 M. tuberculosis clinical isolates from China. 

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Laboratory diagnosis of pulmonary tuberculosis in TB and HIV endemic settings and the contribution of real time PCR for M. tuberculosis in bronchoalveolar lavage …

[PDF] from ru.nl

GS Kibiki, B Mulder… – Tropical Medicine & …, 2007 – Wiley Online Library

 Spoligotyping patterns were analysed with Bionumerics 4.0 (Applied Maths, St-Martin-Latem,
Belgium).  strains in 25 of the 28 BAL samples with positive MTB culture could be spoligotyped.
In all 25, the spoligotypes indicated that the isolates belonged to M. tuberculosis complex 

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[PDF] MYCOBACTERIOSIS IN WILD BOAR: RESULTS OF 2000-2006 ACTIVITY IN NORTH-WESTERN ITALY

[PDF] from vet-alfort.fr

A Dondo, S Zoppi, F Rossi, L Chiavacci, A Barbaro… – aeema.vet-alfort.fr

 37 a PCR based reverse line blot method covalently (Isogen, Netherland). M. bovis strains isolated
were further characterized by VNTR typing (ETR A, B, C, D, E) according to Frothingham
[Frothingham et al., 1998]. The strains unidentified by Spoligotyping were processed by a 

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Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern Ukraine

VV Nikolayevskyy, TJ Brown… – Clinical …, 2007 – Wiley Online Library

 [23] using commercially available membranes (Isogen, Maarssen, The  binding to the
corresponding targets, but an association between these specific spoligotypes and mutations
in  Spoligotyping results demonstrated a relative diversity in the genetic structure of M. tuberculosis 

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Discrepant Results Between Pyrazinamide Susceptibility Testing by the Reference BACTEC 460TB Method and pncA DNA Sequencing in Patients Infected With …

J Dormandy, A Somoskovi, BN Kreiswirth… – Chest, 2007 – chestjournal.chestpubs.org

 Spoligotyping, a polymerase chain reaction-based genotyping method that detects 43 known
spacer sequences that are interspersed between the direct repeats in the genomic repeat region
of M tuberculosis, was performed with a commercially available kit (Isogen Bioscience 

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Új Mycobacterium tuberculosis törzs okozta mikroepidémia budapesti hajléktalanokban

C Ködmön, J Lukács, S Dávid, I Vadász… – Orvosi Hetilap, 2007 – akademiai.com

 A spoligotyping kevésbé érzékeny, mint az IS6110-RFLP fin- gerprint módszer, de kitûnôen
elkülöníthetôk vele a világszer- te  identifikáltuk [5, 6]. A mycobacteriális DNS kinyerését és a
spoligotipizálást ke- reskedelmi forgalomban kapható teszttel (Isogen Bioscience BV 

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Caracterização das mutações envolvidas na resistência de isolados de Mycobacterium tuberculosis à estreptomicina e sua relação com o sistema de efluxo

[PDF] from ufrgs.br

FS Spies – 2007 – lume.ufrgs.br

 48 3.18. Genotipagem dos isolados ….. 48 3.18.1.
Spoligotyping ….. 49 3.18.1.1. Primers….. 49 3.18.1.2. 

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[PDF] Estudo de fatores relacionados ao controle da tuberculose: resistência às drogas, transmissão e suscetibilidade do hospedeiro; Study of factors related to the …

[PDF] from fiocruz.br

MDB Fernandes – 2007 – bdtd.cict.fiocruz.br

 32 Figura 2.8: Representação esquemática do spoligotyping. 35 Figura 2.9: Descrição do
spoligotyping de linhagens das maiores famílias do Mycobacterium tuberculosis. 36 Figura 2.10:
Representação esquemática da seqüência replicada utilizando DRE-PCR. 

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[PDF] PROYECTO DE MODIFICACIÓN A LA NORMA OFICIAL MEXICANA NOM-031-ZOO-1995, CAMPAÑA NACIONAL CONTRA LA TUBERCULOSIS BOVINA ( …

[PDF] from inforural.com.mx

OIE de Salud Animal – inforural.com.mx

Page 1. 54 (Segunda Sección) DIARIO OFICIAL Lunes 3 de diciembre de 2007
SECRETARIA DE AGRICULTURA, GANADERIA, DESARROLLO RURAL, PESCA
Y ALIMENTACION PROYECTO de Modificación a la Norma 

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CARACTERIZAÇÃO DAS MUTAÇÕES ENVOLVIDAS NA RESISTÊNCIA DE ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS À ESTREPTOMICINA E SUA RELAÇÃO COM O SISTEMA DE EFLUXO

[PDF] from ufrgs.br

FS Spies – 2007 – lume.ufrgs.br

 48 3.18. Genotipagem dos isolados ….. 48 3.18.1.
Spoligotyping ….. 49 3.18.1.1. Primers….. 49 3.18.1.2. 

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[PDF] ESTUDO DE FATORES RELACIONADOS AO CONTROLE DA TUBERCULOSE: RESISTÊNCIA ÀS DROGAS, TRANSMISSÃO E SUSCETIBILIDADE DO HOSPEDEIRO; STUDY OF FACTORS RELATED TO THE …

[PDF] from fiocruz.br

MDB Fernandes – 2007 – bdtd.cict.fiocruz.br

 32 Figura 2.8: Representação esquemática do spoligotyping. 35 Figura 2.9: Descrição do
spoligotyping de linhagens das maiores famílias do Mycobacterium tuberculosis. 36 Figura 2.10:
Representação esquemática da seqüência replicada utilizando DRE-PCR. 

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2006

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A case of urinary tuberculosis due toMycobacterium bovissubspeciescaprae

V Sintchenko, P Jelfs, M Dally… – Pathology-Journal of …, 2006 – journals.lww.com

 Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the
MTBC direct repeat  of reference laboratories to differentiate members of the MTBC by spoligotyping,
11 based on  Several spoligotypes have been recognised among M. bovis subsp. 

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… tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping

[HTML] from nih.gov

C Allix, K Walravens, C Saegerman… – Journal of clinical …, 2006 – Am Soc Microbiol

 PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV  IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals  based
on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated 

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Spoligotyping of Mycobacterium tuberculosis isolates from Pakistan reveals predominance of Central Asian Strain 1 and Beijing isolates

[HTML] from nih.gov

Z Hasan, M Tanveer, A Kanji, Q Hasan… – Journal of clinical …, 2006 – Am Soc Microbiol

 positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig.
1). A total of 197 (63%) isolates were grouped into 22 different clusters, while
119 (37%) isolates had unique spoligotypes. Previously 

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Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the samespoligotyping profile as isolates from animals

[HTML] from nih.gov

B Romero, A Aranaz, L de Juan… – Journal of clinical …, 2006 – Am Soc Microbiol

 Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,
The  of techniques is required for appropriate discrimination, even for those spoligotypes that have
 Furthermore, the design of a new spoligotyping membrane that complements the 

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< i> rpoB</i> gene sequencing and spoligotyping of multidrug-resistant< i> Mycobacterium tuberculosis</i> isolates from India

N Suresh, UB Singh, J Arora, H Pant… – Infection, Genetics and …, 2006 – Elsevier

 to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to
identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2.  Spoligotyping.
A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. 

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Usefulness of restriction fragment length polymorphism and spoligotyping for epidemiological studies of< i> Mycobacterium bovis</i> in Madagascar: Description of …

V Rasolofo Razanamparany, R Quirin… – Veterinary …, 2006 – Elsevier

 containing 43 oligonucleotides derived from spacer DNA sequences (obtained from Isogen
Bioscience BV, Utrecht, The Netherlands). PCR and hybridization were performed as described
by Kamerbeek et al. (1997). Spoligotyping patterns or spoligotypes were confirmed by 

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[HTML] Variantes genéticas de Mycobacterium tuberculosis aisladas de pacientes de la Xª Región de Chile

[HTML] from scielo.cl

M Mancilla E, A Martínez H… – Revista chilena de …, 2006 – SciELO Chile

 A significant number of spoligotypes were identical to profiles found in Brazil followed by Argentina
and Spain.  utilizando los partidores biotinilados DRa (5′-GGTTTTGGGTCTGACGAC) y DRb (5′-
CCGAGA GGGGACGGAAAC), incluidos en el sistema de spoligotyping (ISOGEN

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Mycobacterium africanum elicits an attenuated T cell response to early secreted antigenic target, 6 kDa, in patients with tuberculosis and their household contacts

[HTML] from oxfordjournals.org

BC de Jong, PC Hill, RH Brookes… – Journal of Infectious …, 2006 – jid.oxfordjournals.org

 were assessed by spectrophotometry (Eppendorf BioPhotometer). Spoligotyping
was performed using membranes (Isogen Biosciences), in accordance with
standardized methods [19]. Spoligotyping results were scanned and 

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rpoB gene mutations and molecular characterization of rifampin-resistant Mycobacterium tuberculosis isolates from Shandong Province, China

[HTML] from nih.gov

X Ma, H Wang, Y Deng, Z Liu, Y Xu… – Journal of clinical …, 2006 – Am Soc Microbiol

 (20), with data analyzed by Whole Band Analysis software (version 3.2; BioImage,
Ann Arbor, MI). Spoligotyping was carried out by using commercial kits from Isogen
Bioscience BV (Maarssen, The Netherlands). Mycobacterial 

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[HTML] Tuberculosis associated with Mycobacterium tuberculosis Beijing and non-Beijing genotypes: a clinical and immunological comparison

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YJ Sun, TK Lim, A Ong, B Ho… – BMC infectious …, 2006 – biomedcentral.com

 Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, the
Netherlands  The remaining one isolate (N07) was not detected by spoligotyping, but MIRU-VNTR 
Based on spoligotypes and/or MIRU-VNTR patterns, the remaining 20 isolates were 

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Prevalence of Haarlem I and Beijing types of Mycobacterium tuberculosis strains in Iranian and Afghan MDR-TB patients

[PDF] from mrc.ac.ir

P Farnia, MR Masjedi, M Mirsaeidi, F Mohammadi… – Journal of Infection, 2006 – Elsevier

 SpoligotypingSpoligotyping was performed as described by Kamerbeek et al. 5 with a
commercially available kit, according to the instructions supplied by the manufacturer (Isogen
Bioscience BV, Maarsen, The Netherlands).  Distribution of the 263 MDR-TB spoligotypes

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Molecular epidemiology of drug-resistant tuberculosis in Hungary

[HTML] from nih.gov

C Ködmön, S Niemann, J Lukács… – Journal of clinical …, 2006 – Am Soc Microbiol

 described previously (3, 14, 17). Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
instructions of the manufacturer (10). The IS6110 fingerprint and 

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Tuberculosis in mandrills at the Lisbon zoo

A Amado, T Albuquerque, A Gonçalves… – Veterinary …, 2006 – veterinaryrecord.bmj.com

 In order to investigate the infection source, spoligotyping was performed using conventional 43
oligonucleotide Isogen membranes as described by Kamerbeek and others (1997). The two
isolates shared the same spoligotype described as type 237 (Anon 2006). 

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[HTML] Molecular characterisation of Mycobacterium tuberculosis isolates in the First National Survey of Anti-tuberculosis Drug Resistance from Venezuela

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L Aristimuño, R Armengol, A Cebollada… – BMC …, 2006 – biomedcentral.com

 to a set of 43 oligonucleotides covalently bound to the spoligo-membrane (Isogen Biosciences
BV  of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an
International study Global distribution of Mycobacterium tuberculosis spoligotypes

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[PDF] 2. Lipoarabinomannans—structurally diverse and functionally enigmatic macroamphiphiles of mycobacteria and related actinomycetes•

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I Sutcliffe, T Matsumoto, H Ano, T Nagai, K Danno… – 2006 – journals2005.pasteur.ac.ir

 Spoligotyping The clones of M. tuberculosis with less than five IS6110 RFLP bands were subjected
to the second typing, spoligotypingSpoligotyping membranes were purchased from Isogen
bioscience BV (Biltho- ven, The Netherlands). The procedures were 

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Beijing genotype of Mycobacterium tuberculosis is significantly associated with human immunodeficiency virus infection and multidrug resistance in cases of …

[HTML] from nih.gov

M Caws, G Thwaites, K Stepniewska… – Journal of clinical …, 2006 – Am Soc Microbiol

 Spoligotyping.Spoligotyping of isolates was performed according to the standard protocol (6).
Briefly, DNA  Dra (biotin labeled) and DRb, hybridized to the spoligotype membrane (Isogen
Bioscience BV  Spoligotypes were available for isolates from 35 HIV-positive patients (Fig. 

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The most prevalent Mycobacterium tuberculosis superfamilies among Iranian and Afghan TB cases

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AA Velayati, P Farnia, M Mirsaeidi… – … journal of infectious …, 2006 – informahealthcare.com

 [7] with a commercially available kit, according to the instructions supplied by the manufacturer
(Isogen Bioscience BV, Maarsen, The Netherlands).  Spoligotyping  Figure 1. Shows the
predominant spoligotypes which are prevalent among Iranian and Afghan-born immigrants. 

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Extensively drug-resistant tuberculosis: 2 years of surveillance in Iran

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MR Masjedi, P Farnia, S Sorooch… – Clinical infectious …, 2006 – cid.oxfordjournals.org

 [13] with a commercially available kit (Isogen Bioscience).  On the basis of spoligotyping results,
these strains were determined to belong to the East African Indian 3 M. tuberculosis superfamily.
Table 1. Table 1  Distribution of the 12 XDR TB spoligotypes

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